Isolation and Characterization of β-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Mutants of Eschelichia coli and Salmonella enterica Serovar Typhimurium

Journal of Bacteriology

Volumn 186, Issue 6, 2004, Pages 1869-1878

  Lai, Chiou Yan ^a   Cronan, John E ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

3 OXOACYL ACYL CARRIER PROTEIN REDUCTASE; ACYL CARRIER PROTEIN; BACTERIAL ENZYME; MUTANT PROTEIN; OXIDOREDUCTASE; PROTEIN FABG; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TRITIUM; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ISOLATION; ESCHERICHIA COLI; FATTY ACID SYNTHESIS; IN VITRO STUDY; NONHUMAN; PHENOTYPE; POINT MUTATION; PRIORITY JOURNAL; PROTEIN QUATERNARY STRUCTURE; SALMONELLA ENTERICA; TEMPERATURE SENSITIVE MUTANT;

ACYL CARRIER PROTEIN; ALCOHOL OXIDOREDUCTASES; ESCHERICHIA COLI; FATTY ACIDS; GENETIC COMPLEMENTATION TEST; MODELS, MOLECULAR; MUTATION; SALMONELLA TYPHIMURIUM; TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; SALMONELLA; SALMONELLA ENTERICA; SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHIMURIUM;

EID: 1542286897     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.186.6.1869-1878.2004     Document Type: Article

References (41)

1. Alberts, A. W., P. W. Majerus, B. Talamo, and P. R. Vagelos. 1964. Acyl-carrier protein. II. Intermediary reactions of fatty acid synthesis. Biochemistry 3:1563-1571.
2. Anderson, M. S., and C. R. Raetz. 1987. Biosynthesis of lipid A precursors in Escherichia coli. A cytoplasmic acyltransferase that converts UDP-N-acetylglucosamine to UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine. J. Biol. Chem. 262:5159-5169.
3. Ashworth, J. M., H. L. Kornberg, and D. L. Nothmann. 1965. Location of the structural gene for citrate synthase on the chromosome of Escherichia coli K12. J. Mol. Biol. 11:654-657.
4. Balbas, P., X. Soberon, F. Bolivar, and R. L. Rodriguez. 1988. The plasmid, pBR322. Bio/Technology 10:5-41.
5. Benson, N. R., and B. S. Goldman. 1992. Rapid mapping in Salmonella typhimurium with Mud-P22 prophages. J. Bacteriol. 174:1673-1681.
6. Bolotin, A., P. Wincker, S. Mauger, O. Jaillon, K. Malarme, J. Weissenbach, S. D. Ehrlich, and A. Sorokin. 2001. The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403. Genome Res. 11:731-753.
7. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
8. Campos-Garcia, J., A. D. Caro, R. Najera, R. M. Miller-Maier, R. A. Al-Tahhan, and G. Soberon-Chavez. 1998. The Pseudomonas aeruginosa rhlG gene encodes an NADPH-dependent β-ketoacyl reductase which is specifically involved in rhamnolipid synthesis. J. Bacteriol. 180:4442-4451.
9. Clewell, D. B., and D. R. Helinski. 1969. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an open circular DNA form. Proc. Natl. Acad. Sci. USA 62:1159-1166.
10. Datsenko, K. A., and B. L. Wanner. 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. USA 97:6640-6645.
11. Davis, R. W., D. Botstein, and J. R. Roth. 1980. Advanced bacterial genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
12. Davis, T. N., E. D. Muller, and J. E. Cronan, Jr. 1982. The virion of the lipid-containing bacteriophage PR4. Virology 120:287-306.
13. Harder, M. E., I. R. Beacham, J. E. Cronan, Jr., K. Beacham, J. L. Honegger, and D. F. Silbert. 1972. Temperature-sensitive mutants of Escherichia coli requiring saturated and unsaturated fatty acids for growth: isolation and properties. Proc. Natl. Acad. Sci. USA 69:3105-3109.
14. Heath, R. J., and C. O. Rock. 1995. Enoyl-acyl carrier protein reductase (fabl) plays a determinant role in completing cycles of fatty acid elongation in Escherichia coli. J. Biol. Chem. 270:26538-26542.
15. Heath, R. J., and C. O. Rock. 1996. Inhibition of β-ketoacyl-acyl carrier protein synthase III (FabH) by acyl-acyl carrier protein in Escherichia coli. J. Biol. Chem. 271:10996-11000.
16. Heath, R. J., and C. O. Rock. 1996. Roles of the FabA and FabZ [3 P-hydroxyacyl-acyl carrier protein dehydratases in Escherichia coli fatty acid biosynthesis. J. Biol. Chem. 271:27795-27801.
17. Hoang, T. T., S. A. Sullivan, J. K. Cusick, and H. P. Schweizer. 2002. β-Ketoacyl acyl carrier protein reductase (FabG) activity of the fatty acid biosynthetic pathway is a determining factor of 3-oxo-homoserine lactone acyl chain lengths. Microbiology 148:3849-3856.
18. Jackowski, S., and C. O. Rock. 1987. Acetoacetyl-acyl carrier protein synthase, a potential regulator of fatty acid biosynthesis in bacteria. J. Biol. Chem. 262:7927-7931.
19. Jackowski, S., and C. O. Rock. 1981. Regulation of coenzyme A biosynthesis. J. Bacteriol. 148:926-932.
20. Larson, T. J., D. N. Ludtke, and R. M. Bell. 1984. sn-Glycerol-3-phosphate auxotrophy of plsB strains of Escherichia coli: evidence that a second mutation, plsX, is required. J. Bacteriol. 160:711-717.
21. Lopez-Lara, I. M., and O. Geiger. 2001. The nodulation protein NodG shows the enzymatic activity of an 3-oxoacyl-acyl carrier protein reductase. Mol. Plant-Microbe Interact. 14:349-357.
22. Magnuson, K., S. Jackowski, C. O. Rock, and J. E. Cronan, Jr. 1993. Regulation of fatty acid biosynthesis in Escherichia coli. Microbiol. Rev. 57:522-542.
23. Maloy, S. 1990. Experimental techniques in bacterial genetics. Jones and Bartlett Publishers, Boston, Mass.
24. McClelland, M., K. E. Sanderson, J. Spieth, S. W. Clifton, P. Latreille, L. Courtney, S. Porwollik, J. Ali, M. Dante, F. Du, S. Hou, D. Layman, S. Leonard, C. Nguyen, K. Scott, A. Holmes, N. Grewal, E. Mulvaney, E. Ryan, H. Sun, L. Florea, W. Miller, T. Stoneking, M. Nhan, R. Waterston, and R. K. Wilson. 2001. Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature 413:852-856.
25. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
26. Miller, J. H. 1992. A short course in bacterial genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
27. Park, S. J., J. P. Park, and S. Y. Lee. 2002. Metabolic engineering of Escherichia coli for the production of medium-chain-length polyhydroxyalkanoates rich in specific monomers. FEMS Microbiol. Lett. 214:217-222.
28. Post-Beittenmiller, D., J. G. Jaworski, and J. B. Ohlrogge. 1991. In vivo pools of free and acylated acyl carrier proteins in spinach. Evidence for sites of regulation of fatty acid biosynthesis. J. Biol. Chem. 266:1858-1865.
29. Price, A. C., Y. M. Zhang, C. O. Rock, and S. W. White. 2001. Structure of β-ketoacyl-[acyl carrier protein] reductase from Escherichia coli: negative cooperativity and its structural basis. Biochemistry 40:12772-12781.
30. Rawlings, M., and J. E. Cronan, Jr. 1992. The gene encoding Escherichia coli acyl carrier protein lies within a cluster of fatty acid biosynthetic genes. J. Biol. Chem. 267:5751-5754.
31. Ren, Q., N. Sierro, B. Witholt, and B. Kessler. 2000. FabG, an NADPH-dependent 3-ketoacyl reductase of Pseudomonas aeruginosa, provides precursors for medium-chain-length poly-3-hydroxyalkanoate biosynthesis in Escherichia coli. J. Bacteriol. 182:2978-2981.
32. Rock, C. O., and J. E. Cronan. 1996. Escherichia coli as a model for the regulation of dissociable (type II) fatty acid biosynthesis. Biochim. Biophys. Acta 1302:1-16.
33. Silbert, D. F., J. E. Cronan, Jr., I. R. Beacham, and M. E. Harder. 1974. Proceedings: genetic engineering of membrane lipid. Fed. Proc. 33:1725-1732.
34. Singer, M., T. A. Baker, G. Schnitzler, S. M. Deischel, M. Goel, W. Dove, K. J. Jaacks, A. D. Grossman, J. W. Erickson, and C. A. Gross. 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol. Rev. 53:1-24.
35. Smith, H. O. 1968. Defective phage formation by lysogens of integration deficient phage P22 mutants. Virology 34:203-223.
36. Spencer, A., E. Muller, J. E. Cronan, Jr., and T. A. Gross. 1977. relA gene control of the synthesis of lipid A fatty acyl moieties. J Bacteriol 130:114-117.
37. Takeshita, S., M. Sato, M. Toba, W. Masahashi, and T. Hashimoto-Gotoh. 1987. High-copy-number and low-copy-number plasmid vectors for lacZa-complementation and chloramphenicol- or kanamycin-resistance selection. Gene 61:63-74.
38. Toomey, R. E., and S. J. Wakil. 1966. Studies on the mechanism of fatty acid synthesis. XV. Preparation and general properties of β-ketoacyl acyl carrier protein reductase from Escherichia coli. Biochim. Biophys. Acta 116:189-197.
39. Wilson, R. J. 2002. Progress with parasite plastids. J. Mol. Biol. 319:257-274.
40. Youderian, P., P. Sugiono, K. L. Brewer, N. P. Higgins, and T. Elliott. 1988. Packaging specific segments of the Salmonella chromosome with locked-in Mud-P22 prophages. Genetics 118:581-592.
41. Zhang, Y., and J. E. Cronan, Jr. 1998. Transcriptional analysis of essential genes of the Escherichia coli fatty acid biosynthesis gene cluster by functional replacement with the analogous Salmonella typhimurium gene cluster. J Bacteriol 180:3295-3303.