Establishment and utilization of a tetracycline-controlled inducible RNA interfering system to repress gene expression in chronic myelogenous leukemia cells

Acta Biochimica et Biophysica Sinica

Volumn 37, Issue 12, 2005, Pages 851-856

  Yang, Fan ^a   Zhang, Yun ^a   Cao, Ying Li ^a   Wang, Shu Hui ^a   Liu, Li ^a  

^a PEKING UNION MEDICAL COLLEGE   (China)

Author keywords

CRLF3; Inducible siRNA; Reverse tetracycline controlled transactivator; Tetracycline; U6 promoter

Indexed keywords

CRLF3 PROTEIN, HUMAN; CYTOKINE RECEPTOR; SMALL INTERFERING RNA; SMALL NUCLEAR RNA; TETRACYCLINE DERIVATIVE; TRANSACTIVATOR PROTEIN; U6 SMALL NUCLEAR RNA;

ARTICLE; CELL STRAIN K 562; CHEMISTRY; CHRONIC MYELOID LEUKEMIA; DNA RESPONSIVE ELEMENT; GENE EXPRESSION REGULATION; GENE TARGETING; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; HUMAN; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROMOTER REGION; RNA INTERFERENCE;

BASE SEQUENCE; GENE EXPRESSION REGULATION; GENE TARGETING; GENETIC VECTORS; HUMANS; K562 CELLS; LEUKEMIA, MYELOID, CHRONIC; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS (GENETICS); RECEPTORS, CYTOKINE; RESPONSE ELEMENTS; RNA INTERFERENCE; RNA, SMALL INTERFERING; RNA, SMALL NUCLEAR; TETRACYCLINES; TRANS-ACTIVATORS; TRANSFECTION;

EID: 33644876259     PISSN: 16729145     EISSN: 17457270     Source Type: Journal    
DOI: 10.1111/j.1745-7270.2005.00112.x     Document Type: Article

References (27)

1. Sharp PA. RNA interference-2001. Genes Dev 2001, 15: 485-490
2. Meister G, Tuschl T. Mechanisms of gene silencing by double-stranded RNA. Nature 2004, 431: 343-349
3. Borkhardt A, Heidenreich O. RNA interference as a potential tool in the treatment of leukaemia. Expert Opin Biol Ther 2004, 4: 1921-1929
4. Rowley JD. A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining [letter]. Nature 1973, 243: 290-293
5. Laurent E, Talpaz M, Kantarjian H, Kurzrock R. The BCR gene and Philadelphia chromosome-positive leukemogenesis. Cancer Res 2001, 61: 2343-2355
6. Wilda M, Fuchs U, Wossmann W, Borkhardt A. Killing of leukemic cells with a BCR/ABL fusion gene by RNA interference (RNAi). Oncogene 2002, 21: 5716-5724
7. Hariharan IK, Adams JM, Cory S. bcr-abl oncogene renders myeloid cell line factor independent: Potential autocrine mechanism in chronic myeloid leukemia. Oncogene Res 1988, 3: 387-399
8. Nieborowska-Skorska M, Wasik MA, Slupianek A, Salomoni P, Kitamura T, Calabretta B, Skorski T. Signal transducer and activator of transcription (STAT)5 activation by BCR/ABL is dependent on intact Src homology (SH) 3 and SH2 domains of BCR/ABL and is required for leukemogenesis. J Exp Med 1999, 189: 1229-1242
9. Klucher KM, Lopez DV, Daley GQ. Secondary mutation maintains the transformed state in BaF3 cells with inducible BCR/ABL expression. Blood 1998, 91: 3927-3934
10. Scherr M, Battmer K, Winkler T, Heidenreich O, Ganser A, Eder M. Specific inhibition of bcr-abl gene expression by small interfering RNA. Blood 2003, 101: 1566-1569
11. Scherr M, Battmer K, Schultheis B, Ganser A, Eder M. Stable RNA interference (RNAi) as an option for anti-bcr-abl therapy. Gene Ther 2005, 12: 12-21
12. Ohkawa J, Taira K. Control of the functional activity of an antisense RNA by a tetracycline-responsive derivative of the human U6 snRNA promoter. Hum Gene Ther 2000, 11: 577-585
13. Shi H, Djikeng A, Mark T, Wirtz E, Tschudi C, Ullu E. Genetic interference in Trypanosoma brucei by heritable and inducible double-stranded RNA. RNA 2000, 6: 1069-1076
14. Gelman MS, Ye XK, Stull R, Suhy D, Jin L, Ng D, Than B et al. Identification of cell surface and secreted proteins essential for tumor cell survival using a genetic suppressor element screen. Oncogene 2004, 23: 8158-8170
15. Wang S, El-Deiry WS. Inducible silencing of KILLER/DR5 in vivo promotes bioluminescent colon tumor xenograft growth and confers resistance to chemotherapeutic agent 5-fluorouracil. Cancer Res 2004, 64: 6666-6672
16. Higuchi M, Tsutsumi R, Higashi H, Hatakeyama M. Conditional gene silencing utilizing the lac repressor reveals a role of SHP-2 in cagA-positive Helicobacter pylori pathogenicity. Cancer Sci 2004, 95: 442-447
17. Chen Y, Stamatoyannopoulos G, Song CZ. Down-regulation of CXCR4 by inducible small interfering RNA inhibits breast cancer cell invasion in vitro, Cancer Res 2003, 63: 4801-4804
18. Czauderna F, Santel A, Hinz M, Fechtner M, Durieux B, Fisch G, Leenders F et al. Inducible shRNA expression for application in a prostate cancer mouse model. Nucleic Acids Res 2003, 31: e127
19. Gossen M, Bujard H. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci USA 1992, 89: 5547-5551
20. McGuire SE, Roman G, Davis RL. Gene expression systems in Drasophila: A synthesis of time and space. Trends Genet 2004, 20: 384-391
21. Stevenson M. Therapeutic potential of RNA interference. N Engl J Med 2004, 351: 1772-1777
22. Teixeira SM, daRocha WD. Control of gene expression and genetic manipulation in the Trypanosomatidae. Genet Mol Res 2003, 2: 148-158
23. Paule MR, White RJ. Survey and summary: Transcription by RNA polymerases I and III. Nucleic Acids Res 2000, 28: 1283-1298
24. Myslinski E, Ame JC, Krol A, Carbon P. An unusually compact external promoter for RNA polymerase III transcription of the human H1RNA gene. Nucleic Acids Res 2001, 29: 2502-2509
25. van de Wetering M, Oving I, Muncan V, Pon Fong MT, Brantjes H, van Leenen D, Holstege FC et al. Specific inhibition of gene expression using a stably integrated, inducible small-interfering-RNA vector. EMBO Rep 2003, 4: 609-615
26. Matsukura S, Jones PA, Takai D. Establishment of conditional vectors for hairpin siRNA knockdowns. Nucleic Acids Res 2003, 31: e77
27. Negeri D, Eggert H, Gienapp R, Saumweber H. Inducible RNA interference uncovers the Drosophila protein Bx42 as an essential nuclear cofactor involved in Notch signal transduction. Mech Dev 2002, 117: 151-162