A high-throughput screen for genes from castor that boost hydroxy fatty acid accumulation in seed oils of transgenic Arabidopsis

Plant Journal

Volumn 45, Issue 5, 2006, Pages 847-856

  Lu, Chaofu ^a,b   Fulda, Martin ^a,c   Wallis, James G ^a   Browse, John ^a  

^a WASHINGTON STATE UNIVERSITY   (United States)

^b MONTANA STATE UNIVERSITY   (United States)

^c UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

Arabidopsis; Castor; Full length cDNA library; High throughput; Ricinoleate; Transgenic plants

Indexed keywords

DNA; FATTY ACIDS; GENES; PLANTS (BOTANY); SEED; THROUGHPUT;

ARABIDOPSIS; CASTOR; FULL-LENGTH CDNA LIBRARY; HIGH-THROUGHPUT; RICINOLEATE; TRANSGENIC PLANTS;

GENETIC ENGINEERING;

FATTY ACID;

ARABIDOPSIS; ARTICLE; BIOLISTIC TRANSFORMATION; BIOSYNTHESIS; GAS CHROMATOGRAPHY; GENE; GENE LIBRARY; GENE VECTOR; GENETIC PROCEDURES; GENETIC TRANSFORMATION; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR CLONING; PHYSIOLOGY; PLANT SEED; RICINUS COMMUNIS; TRANSGENIC PLANT;

ARABIDOPSIS; BIOLISTICS; CASTOR BEAN; CHROMATOGRAPHY, GAS; CLONING, MOLECULAR; FATTY ACIDS; GENE LIBRARY; GENES, PLANT; GENETIC VECTORS; MOLECULAR PROBE TECHNIQUES; PLANTS, GENETICALLY MODIFIED; SEEDS; TRANSFORMATION, GENETIC;

GENES; GENETIC ENGINEERING; PLANTS; THROUGHPUT;

ARABIDOPSIS; ARABIDOPSIS THALIANA;

EID: 33644867728     PISSN: 09607412     EISSN: 1365313X     Source Type: Journal    
DOI: 10.1111/j.1365-313X.2005.02636.x     Document Type: Article

References (32)

1. Allen G.C. Spiker S. Thompson W.F. 2000 Use of matrix attachment regions (MARs) to minimize transgene silencing Plant Mol. Biol. 43 361 376
2. Bafor M. Smith M.A. Jonsson L. Stobart K. Stymne S. 1991 Ricinoleic acid biosynthesis and triacylglycerol assembly in microsomal preparations from developing castor bean (Ricinus communis) endosperm Biochem. J. 280 507 514
3. Broun P. Somerville C. 1997 Accumulation of ricinoleic, lesquerolic, and densipolic acids in seeds of transgenic Arabidopsis plants that express a fatty acyl hydroxylase cDNA from castor bean Plant Physiol. 113 933 942
4. Browse J. Somerville C. 1991 Glycerolipid synthesis: biochemistry and regulation Annu. Rev. Plant Physiol. Plant Mol. Biol. 42 467 506
5. Cahoon E.B. Carlson T.J. Ripp K.G. Schweiger B.J. Cook G.A. Hall S.E. Kinney A.J. 1999 Biosynthetic origin of conjugated double bonds: production of fatty acid components of high-value drying oils in transgenic soybean embryos Proc. Natl Acad. Sci. USA 96 12935 12940
6. Capell T. Christou P. 2004 Progress in plant metabolic engineering Curr. Opin. Biotechnol. 15 148 154
7. Carninci P. Hayashizaki Y. 1999 High efficiency full-length cDNA cloning Methods Enzymol. 303 19 44
8. Carninci P. Kvam C. Kitamura A. et al. 1996 High-efficiency full-length cDNA cloning by biotinylated CAP trapper Genomics 37 327 336
9. Carninci P. Nishiyama Y. Westover A. Itoh M. Nagaoka S. Sasaki N. Okazaki Y. Muramatsu M. Hayashizaki Y. 1998 Thermostabilization and thermoactivation of thermolabile enzymes by trehalose and its application for the synthesis of full length cDNA Proc. Natl Acad. Sci. USA 95 520 524
10. Carninci P. Shibata Y. Hayatsu N. Sugahara Y. Shibata K. Itoh M. Konno H. Okazaki Y. Muramatsu M. Hayashizaki Y. 2000 Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes Genome Res. 10 1617 1630
11. Clough S.J. Bent A.F. 1998 Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana Plant J. 16 735 743
12. Greenwood J.S. Bewley J.D. 1982 Seed development in Ricinus communis (castor bean). I. Descriptive morphology Can. J. Bot. 60 1751 1760
13. Jaworski J. Cahoon E.B. 2003 Industrial oils from transgenic plants Curr. Opin. Plant Biol. 6 178 184
14. Lee M. Lenman M. Banas A. et al. 1998 Identification of non-heme di-iron proteins that catalyze triple bond and epoxy group formation Science 280 915 918
15. Lin J.T. Lew K.M. Chen J.M. Iwasaki Y. McKeon T.A. 2000 Metabolism of 1-acyl-2-oleoyl-sn-glycero-3-phosphoethanolamine in castor oil biosynthesis Lipids 35 481 486
16. van de Loo F.J. Broun P. Turner S. Somerville C. 1995 An oleate 12-hydroxylase from Ricinus communis L. is a fatty acyl desaturase homolog Proc. Natl Acad. Sci. USA 92 6743 6747
17. Lukowitz W. Gillmor C.S. Scheible W.R. 2000 Positional cloning in Arabidopsis. Why it feels good to have a genome initiative working for you Plant Physiol. 123 795 805
18. Millar A.A. Smith M.A. Kunst L. 2000 All fatty acids are not equal: discrimination in plant membrane lipids Trends Plant Sci. 5 95 101
19. Muller K.D. Nalik H.P. Schmid E.N. Husmann H. Schomburg G. 1993 Fast identification of Mycobacterium species by GC analysis with trimethylsulfonium hydroxide (TMSH) for transesterification J. High Resolut. Chromatogr. 16 161 165
20. Okuley J. Lightner J. Feldmann K. Yadav N. Lark E. Browse J. 1994 Arabidopsis Fad2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis Plant Cell 6 147 158
21. Petersen K. Leah R. Knudsen S. Cameron-Mills V. 2002 Matrix attachment regions (MARs) enhance transformation frequencies and reduce variance of transgene expression in barley Plant Mol. Biol. 49 45 58
22. Sengupta-Gopalan C. Reichert N.A. Barker R.F. Hall T.C. Kemp J.D. 1985 Developmentally regulated expression of the β-phaseolin gene in tobacco seed Proc. Natl Acad. Sci. USA 82 3320 3324
23. Shibata Y. Carninci P. Watahiki A. Shiraki T. Konno H. Muramatsu M. Hayashizaki Y. 2001 Cloning full-length, cap-trapper-selected cDNAs by using the single-strand linker ligation method BioTechniques 30 1250 1254
24. Short J.M. Fernandez J.M. Sorge J.A. Huse W.D. 1988 Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties Nucleic Acids Res. 16 7583 7600
25. Singh S.P. Zhou X.R. Liu Q. Stymne S. Green A.G. 2005 Metabolic engineering of new fatty acids in plants Curr. Opin. Plant Biol. 8 197 203
26. Smith M.A. Moon H. Chowrira G. Kunst L. 2003 Heterologous expression of a fatty acid hydroxylase gene in developing seeds of Arabidopsis thaliana Planta 217 507 516
27. Spiess A.N. Ivell R. 2002 A highly efficient method for long-chain cDNA synthesis using trehalose and betaine Anal. Biochem. 301 168 174
28. Stuitje A.R. Verbree E.C. van der Linden K.H. Mietkiewska E.M. Nap J.P. Kneppers T.J.A. 2003 Seed-expressed fluorescent proteins as versatile tools for easy (co)transformation and high-throughput functional genomics in Arabidopsis Plant Biotechnol. J. 1 301 309
29. Suh M.C. Schultz D.J. Ohlrogge J.B. 2002 What limits production of unusual monoenoic fatty acids in transgenic plants Planta 215 584 595
30. Verdaguer B. de Kochko A. Beachy R.N. Fauquet C. 1996 Isolation and expression in transgenic tobacco and rice plants, of the cassava vein mosaic virus (CVMV) promoter Plant Mol. Biol. 31 1129 1139
31. Weselake R.J. 2005 Storage lipids In Plant Lipids - Biology, Utilization and Manipulation Murphy D.J. ed. Oxford, UK Blackwell pp 162 225
32. Zeng G. 1998 Sticky-end PCR: new method for subcloning BioTechniques 25 206 208