The main catechin of green tea, (-)-epigallocatechin-3-gallate (EGCG), reduces bleomycin-induced DNA damage in human leucocytes

Toxicology in Vitro

Volumn 20, Issue 3, 2006, Pages 295-300

  Glei, M ^a   Pool Zobel, B L ^a  

^a FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

Author keywords

Comet assay; DNA damage; EGCG; Human leucocytes

Indexed keywords

BLEOMYCIN; CATECHIN; DEOXYRIBONUCLEASE; DEOXYRIBONUCLEASE III; EPIGALLOCATECHIN GALLATE; GREEN TEA EXTRACT; PHYTOHEMAGGLUTININ; SCAVENGER; UNCLASSIFIED DRUG;

ARTICLE; CELL ISOLATION; CELL PROTECTION; COMET ASSAY; CONTROLLED STUDY; DETOXIFICATION; DNA DAMAGE; DNA INTEGRATION; DNA REPAIR; GENOTOXICITY; HUMAN; HUMAN CELL; HYPOTHESIS; LEUKOCYTE; TEA;

ANTIBIOTICS, ANTINEOPLASTIC; BLEOMYCIN; CATECHIN; COMET ASSAY; DEOXYRIBONUCLEASES, TYPE III SITE-SPECIFIC; DNA DAMAGE; HUMANS; LEUKOCYTES; PHYTOHEMAGGLUTININS;

EID: 33644786885     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tiv.2005.08.002     Document Type: Article

References (29)

1. R.F. Anderson, L.J. Fisher, Y. Hara, T. Harris, W.B. Mak, L.D. Melton, and J.E. Packer Green tea catechins partially protect DNA from OH radical induced strand breaks and base damage through fast chemical repair of DNA radicals Carcinogenesis 22 2001 1189 1193
2. S. Azam, N. Hadi, N.U. Khan, and S.M. Hadi Prooxidant property of green tea polyphenols epicatechin and epigallocatechin-3-gallate: implications for anticancer properties Toxicology in Vitro 18 2004 555 561
3. R. Benelli, R. Vene, D. Bisacchi, S. Garbisa, and A. Albini Anti-invasive effects of green tea polyphenol epigallocatechin-3-gallate (EGCG), a natural inhibitor of metallo and serine proteases Biological Chemistry 383 2002 101 105
4. B. Bertram, U. Bollow, N. Rajaee-Behbahani, A. Burkle, and P. Schmezer Induction of poly(ADP-ribosyl)ation and DNA damage in human peripheral lymphocytes after treatment with (-)-epigallocatechin-gallate Mutation Research 534 2003 77 84
5. A. Boyum Isolation of mononuclear cells and granulocytes from human blood Scandinavian Journal of Clinical and Laboratory Investigation 21 1968 77
6. A. Bub, B. Watzl, M. Blockhaus, K. Briviba, U.M. Liegibel, H. Müller, B.L. Pool-Zobel, and G. Rechkemmer Fruit juice consumption modulates antioxidative status, immune status and DNA damage Journal of Nutritional Biochemistry 14 2003 98
7. H. Cederberg, and C. Ramel Modifications of the effect of bleomycin in the somatic mutation and recombination test in Drosophila melanogaster Mutation Research 214 1989 69 80
8. A.R. Collins The comet assay for DNA damage and repair: principles, applications, and limitations Molecular Biotechnology 26 2004 249 261
9. A.R. Collins, V. Harrington, J. Drew, and R. Melvin Nutritional modulation of DNA repair in a human intervention study Carcinogenesis 24 2003 511 515
10. A. Dhawan, D. Anderson, S. de Pascual-Teresa, C. Santos-Buelga, M.N. Clifford, and C. Ioannides Evaluation of the antigenotoxic potential of monomeric and dimeric flavanols, and black tea polyphenols against heterocyclic amine-induced DNA damage in human lymphocytes using the Comet assay Mutation Research 515 2002 39 56
11. M. Glei, U.M. Liegibel, M.N. Ebert, V. Böhm, and B.L. Pool-Zobel β-Carotene reduces bleomycin-induced genetic damage in human lymphocytes Toxicology and Applied Pharmacology 179 2002 65 73
12. M. Glei, M. Matuschek, C. Steiner, V. Böhm, C. Persin, and B.L. Pool-Zobel Initial in vitro toxicity testing of functional foods rich in catechins and anthocyanins in human cells Toxicology in Vitro 17 2003 723 729
13. S.M. Henning, Y. Niu, N.H. Lee, G.D. Thames, R.R. Minutti, H. Wang, V.L. Go, and D. Heber Bioavailability and antioxidant activity of tea flavanols after consumption of green tea, black tea, or a green tea extract supplement American Journal of Clinical Nutrition 80 2004 1558 1564
14. T.C. Hsu, Y.J. Zhao, R.Y. Wang, Y.F. Wu, M.R. Spitz, and W.K. Hong Comparative efficacy as antioxidants between ascorbic acid and epigallocatechin gallate on cells of two human lymphoblastoid lines Cancer Genetics and Cytogenetics 124 2001 169 171
15. E. Kaminskas, and J.C. Li Repair of DNA damage induced by oxygen radicals in human non-proliferating and proliferating lymphocytes Mutation Research 274 1992 103 110
16. C. Krul, A. Luiten-Shuite, A. Tenfelde, B. van Ommen, H. Verhagen, and R. Havenaar Antimutagenic activity of green tea and black tea extracts studied in a dynamic in vitro gastrointestinal model Mutation Research 474 2001 71 85
17. J.D. Lambert, and C.S. Yang Cancer chemopreventive activity and bioavailability of tea and tea polyphenols Mutation Research 523-524 2003 201 208
18. M.-J. Lee, Z.-Y. Wang, H. Li, L. Chen, Y. Sun, S. Gobbo, D.A. Balentine, and C.S. Yang Analysis of plasma and urinary tea polyphenols in human subjects Cancer Epidemiology, Biomarkers and Prevention 4 1995 393 399
19. B.L. Pool-Zobel, A. Bub, H. Müller, I. Wollowski, and G. Rechkemmer Consumption of vegetables reduces genetic damage in humans: first results of an intervention trial with carotenoid-rich foods Carcinogenesis 18 1997 1847 1850
20. C.A. Rice-Evans, N.J. Miller, and G. Paganga Structure-antioxidant activity relationships of flavonoids and phenolic acids Free Radical Biology and Medicine 20 1996 933 956
21. J.C. Rockett, M.E. Burczynski, A.J. Fornace, P.C. Herrmann, S.A. Krawetz, and D.J. Dix Surrogate tissue analysis: monitoring toxicant exposure and health status of inaccessible tissues through the analysis of accessible tissues and cells Toxicology and Applied Pharmacology 194 2004 189 199
22. M. Roy, S. Chakrabarty, D. Sinha, R.K. Bhattacharya, and M. Siddiqi Anticlastogenic, antigenotoxic and apoptotic activity of epigallocatechin gallate: a green tea polyphenol Mutation Research 523-524 2003 33 41
23. P. Schmezer, N. Rajaee-Behbahani, A. Risch, St. Thiel, W. Rittgen, P. Drings, H. Dienemann, K.W. Kayser, V. Schulz, and H. Bartsch Rapid screening assay for mutagen sensitivity and DNA repair capacity in human peripheral blood lymphocytes Mutagenesis 16 2001 25 30
24. R.R. Tice, E. Agurell, D. Anderson, B. Burlinson, A. Hartmann, H. Kobayashi, Y. Miyamae, E. Rojas, J.C. Ryu, and Y.F. Sasaki Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing Environmental and Molecular Mutagenesis 35 2000 206 221
25. H. Verhagen, O.I. Aruoma, J.H.M. van Delft, L.O. Dragsted, L.R. Ferguson, S. Knasmüller, B.L. Pool-Zobel, H.E. Poulson, G. Williamson, and S. Yannai The 10 basic requirements for a scientific paper reporting antioxidant, antimutagenic or anticarcinogenic potential of test substances in in vitro experiments and animal studies in vivo Food and Chemical Toxicology 41 2003 603 610
26. T.M. Vollberg, K.A. Lee, and M.A. Sirover Positive correlation between the extent of cell proliferation and the regulation of base excision repair Cancer Research 44 1984 2377 2381
27. Z.Y. Wang, M. Das, D.R. Bickers, and H. Mukhtar Interaction of epicatechins derived from green tea with rat hepatic cytochrome p-450 Drug Metabolism and Disposition 16 1988 98 103
28. J.Z. Xu, S.Y. Yeung, Q. Chang, Y. Huang, and Z.Y. Chen Comparison of antioxidant activity and bioavailability of tea epicatechins with their epimers British Journal of Nutrition 91 2004 873 881
29. G.C. Yen, J.W. Ju, and C.H. Wu Modulation of tea and tea polyphenols on benzo(a)pyrene-induced DNA damage in Chang liver cells Free Radical Research 38 2004 193 200