Analysis of RNA-protein interactions by a microplate-based fluorescence anisotropy assay

Analytical Biochemistry

Volumn 350, Issue 2, 2006, Pages 222-232

  Mao, Chengjian ^a   Flavin, Kathryn Goolsby ^a   Wang, Stanley ^a   Dodson, Robin ^a   Ross, Jeffrey ^b   Shapiro, David J ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

c myc mRNA; CRD BP; Fluorescence anisotropy fluorescence polarization; Microplate based FA assay; RNA protein interactions; Vigilin; Vitellogenin mRNA

Indexed keywords

ANISOTROPY; FLUORESCENCE; PLANTS (BOTANY); POLARIZATION; PROBES; PROTEINS;

CRD-BP; FLUORESCENCE ANISOTROPY; MICROPLATES; RNA-PROTEIN INTERACTIONS; VIGILIN; VITELLOGENIN MRNA;

RNA;

ALCOHOL; DIMETHYL SULFOXIDE; FLUORESCEIN; HOE 33342; METHANOL; MYC PROTEIN; NEOMYCIN; RNA BINDING PROTEIN; SINGLE STRANDED DNA; SINGLE STRANDED RNA; UNCLASSIFIED DRUG; VIGILIN; VITELLOGENIN;

3' UNTRANSLATED REGION; ANIMAL CELL; ARTICLE; BINDING AFFINITY; CELL FUNCTION; CELL VIABILITY; CONTROLLED STUDY; DROSOPHILA; FLUORESCENCE POLARIZATION IMMUNOASSAY; HETEROCHROMATIN; HIGH THROUGHPUT SCREENING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DNA BINDING; PROTEIN RNA BINDING; REPRODUCIBILITY; RNA DEGRADATION; RNA STABILITY;

ANIMALIA;

EID: 33644674229     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.12.010     Document Type: Article

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