Does the myosin V neck region act as a lever?

Journal of Muscle Research and Cell Motility

Volumn 25, Issue 1, 2004, Pages 29-35

  Moore, Jeffrey R ^a   Krementsova, Elena B ^a   Trybus, Kathleen M ^a   Warshaw, David M ^a  

^a UNIVERSITY OF VERMONT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; CALMODULIN; EPITOPE; MYOSIN V; PYROXYLIN;

ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; CHICKEN; CORRELATION ANALYSIS; GENE CONSTRUCT; INSECT CELL; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN MOTIF;

ACTINS; ANIMALS; GENE EXPRESSION; LASERS; MUSCLE CONTRACTION; MUTATION; MYOSIN TYPE V; PROTEIN TRANSPORT; RECOMBINANT PROTEINS; TIME FACTORS;

GALLUS GALLUS; INSECTA; UNIDENTIFIED BACULOVIRUS;

EID: 3343016961     PISSN: 01424319     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:JURE.0000021394.48560.71     Document Type: Article

References (46)

1. Anson M, Geeves MA, Kurzawa SE and Manstein DJ (1996) Myosin motors with artificial lever arms. EMBO J 15: 6069-6074.
2. Burgess S, Walker M, Wang F, Sellers JR, White HD, Knight PJ and Trinick J (2002) The prepower stroke conformation of myosin V. J Cell Biol 159: 983-991.
3. Dominguez R, Freyzon Y, Trybus KM and Cohen C (1998) Crystal structure of a vertebrate smooth muscle myosin motor domain and its complex with the essential light chain: visualization of the prepower stroke state. Cell 94: 559-571.
4. Finer JT, Mehta AD and Spudich JA (1995) Characterization of single actin-myosin interactions. Biophys J 68: 291S-296S.
5. Forkey JN, Quinlan ME, Shaw MA, Corrie JE and Goldman YE (2003) Three-dimensional structural dynamics of myosin V by single-molecule fluorescence polarization. Nature 422: 399-404.
6. Guilford WH, Dupuis DE, Kennedy G, Wu J, Patlak JB and Warshaw DM (1997) Smooth muscle and skeletal muscle myosins produce similar unitary forces and displacements in the laser trap. Biophys J 72: 1006-1021.
7. Houdusse A, Kalabokis VN, Himmel D, Szent-Gyorgyi AG and Cohen C (1999) Atomic structure of scallop myosin subfragment S1 complexed with MgADP: a novel conformation of the myosin head. Cell 97: 459-470.
8. Houdusse A, Szent-Gyorgyi AG and Cohen C (2000) Three conformational states of scallop myosin S1. Proc Natl Acad Sci USA 97: 11238-11243.
9. Howard J (2001) Mechanics of Motor Proteins and the Cytoskeleton. Sinauer Associates, Inc., Sunderland, MA. p. 145.
10. Howard J and Spudich JA (1996) Is the lever arm of myosin a molecular elastic element? Proc Natl Acad Sci USA 93: 4462-4464.
11. Huxley HE (1990) Sliding filaments and molecular motile systems. J Biol Chem 265: 8347-8350.
12. Kad NM, Rovner AS, Fagnant PM, Joel PB, Kennedy G, Patlak JB, Warshaw D and Trybus KM (2003) A mutant heterodimeric myosin with one inactive head generates maximal displacement. J Cell Biol, in press.
13. Kohler D, Ruff C, Meyhofer E and Bahler M (2003) Different degrees of lever arm rotation control myosin step size. J. Cell Biol. 161: 237-241.
14. Kollmar M, Durrwang U, Kliche W, Manstein DJ and Kull FJ (2002) Crystal structure of the motor domain of a class-I myosin. EMBO J 21: 2517-2525.
15. Lowey S, Waller GS and Bandman E (1991) Neonatal and adult myosin heavy chains form homodimers during avian skeletal muscle development. J Cell Biol 113: 303-310.
16. Mehta A (2001) Myosin learns to walk. J Cell Sci 114: 1981-1998.
17. Mehta AD, Rock RS, Rief M, Spudich JA, Mooseker MS and Cheney RE (1999) Myosin-V is a processive actin-based motor. Nature 400: 590-593.
18. Mercer JA, Seperack PK, Strobel MC, Copeland NG and Jenkins NA (1991) Novel myosin heavy chain encoded by murine dilute coat colour locus. Nature 349: 709-713.
19. Mermall V, Post PL and Mooseker MS (1998) Unconventional myosins in cell movement, membrane traffic, and signal transduction. Science 279: 527-533.
20. Molloy JE, Burns JE, Kendrick-Jones J, Tregear RT and White DC (1995) Movement and force produced by a single myosin head. Nature 378: 209-212.
21. Moore LA, Arrizubieta MJ, Tidyman WE, Herman LA and Bandman E (1992) Analysis of the chicken fast myosin heavy chain family. Localization of isoform-specific antibody epitopes and regions of divergence. J Mol Biol 225: 1143-1151.
22. Moore JR, Krementsova EB, Trybus KM and Warshaw DM (2001) Myosin V exhibits a high duty cycle and large unitary displacement. J Cell Biol 155: 625-635.
23. Moore JR, Krementsova E, Trybus KM and Warshaw DM (2002) Molecular mechanics of expressed-monomeric myosin V fragments with different neck lengths. Biophys J 82(1): 409a.
24. O'Reilly DR, Miller LK and Luckow VA (1992) Baculovirus Expression Veclors. A Laboratory Manual. W.H. Freeman and Co., New York.
25. Purcell TJ, Morris C, Spudich JA and Sweeney HL (2002) Role of the lever arm in the processive stepping of myosin V. Proc Natl Acad Sci USA 99: 14159-14164.
26. Rock RS, Rice SE, Wells AL, Purcell TJ, Spudich JA and Sweeney HL (2001) Myosin VI is a processive motor with a large step size. Proc Natl Acad Sci USA 98: 13655-13659.
27. Ruff C, Furch M, Brenner B, Manstein DJ and Meyhofer E (2001) Single-molecule tracking of myosins with genetically engineered amplifier domains. Nat Struct Biol 8: 226-229.
28. Sakamoto T, Wang F, Schmitz S, Xu Y, Molloy JE, Veigel C and Sellers JR (2003) Neck length and processivity of myosin V. J Biol Chem 278: 29201-29207.
29. Schott DH, Collins RN and Bretscher A (2002) Secretory vesicle transport velocity in living cells depends on the myosin-V lever arm length. J Cell Biol 156: 35-39.
30. Shih WM, Gryczynski Z, Lakowicz JR and Spudich JA (2000) A FRET-based sensor reveals large ATP hydrolysis-induced conformational changes and three distinct states of the molecular motor myosin. Cell 102: 683-694.
31. Tanaka H, Homma K, Iwane AH, Katayama E, Ikebe R, Saito J, Yanagida T and Ikebe M (2002) The motor domain determines the large step of myosin-V. Nature 415: 192-195.
32. Trybus KM (1994) Regulation of expressed truncated smooth muscle myosins. Role of the essential light chain and tail length. J Biol Chem 269: 20819-20822.
33. Trybus KM, Freyzon Y, Faust LZ and Sweeney HL (1997) Spare the rod, spoil the regulation: necessity for a myosin rod. Proc Natl Acad Sci USA 94: 48-52.
34. Trybus KM, Krementsova E and Freyzon Y (1999) Kinetic characterization of a monomeric unconventional myosin V construct. J Biol Chem 274: 27448-27456.
35. Tyska MJ and Warshaw DM (2002) The myosin power stroke. Cell Motil Cytoskeleton 51: 1-15.
36. Tyska MJ, Dupuis DE, Guilford WH, Patlak JB, Waller GS, Trybus KM, Warshaw DM and Lowey S (1999) Two heads of myosin are better than one for generating force and motion. Proc Natl Acad Sci USA 96: 4402-4407.
37. Uyeda TQ, Abramson PD and Spudich JA (1996) The neck region of the myosin motor domain acts as a lever arm to generate movement. Proc Natl Acad Sci USA 93: 4459-4464.
38. Veigel C, Bartoo ML, White DC, Sparrow JC and Molloy JE (1998) The stiffness of rabbit skeletal actomyosin cross-bridges determined with an optical tweezers. Biophys J 75: 1424-1438.
39. Veigel C, Wang F, Bartoo ML, Sellers JR and Molloy JE (2002) The gated gait of the processive molecular motor, myosin V. Nat Cell Biol 4: 59-65.
40. Walker ML, Burgess SA, Sellers JR, Wang F, Hammer JA, III, Trinick J and Knight PJ (2000) Two-headed binding of a processive myosin to F-actin. Nature 405: 804-807.
41. Warshaw DM, Desrosiers JM, Work SS and Trybus KM (1990) Smooth muscle myosin cross-bridge interactions modulate actin filament sliding velocity in vitro. J Cell Biol 111: 453-463.
42. Warshaw DM, Guilford WH, Freyzon Y, Krementsova E, Palmiter KA, Tyska MJ, Baker JE and Trybus KM (2000) The light chain binding domain of expressed smooth muscle heavy meromyosin acts as a mechanical lever. J Biol Chem 275: 37167-37172.
43. Wells JA and Yount RG (1979) Active site trapping of nucleotides by crosslinking two sulfhydryls in myosin subfragment. Proc Natl Acad Sci USA 76: 4966-4970.
44. Work SS and Warshaw DM (1992) Computer-assisted tracking of actin filament motility. Anal Biochem 202: 275-285.
45. Yanagida T, Kitamura K, Tanaka H, Hikikoshi IA and Esaki S (2000) Single molecule analysis of the actomyosin motor. Curr Opin Cell Biol 12: 20-25.
46. Yengo CM, De la Cruz EM, Safer D, Ostap EM and Sweeney HL (2002) Kinetic characterization of the weak binding states of myosin V. Biochemistry 41: 8508-8517.