Myosin V exhibits a high duty cycle and large unitary displacement

Journal of Cell Biology

Volumn 155, Issue 3, 2001, Pages 625-635

  Moore, Jeffrey R ^a   Krementsova, Elena B ^a   Trybus, Kathleen M ^a   Warshaw, David M ^a  

^a UNIVERSITY OF VERMONT   (United States)

Author keywords

In vitro motility; Laser trap; Mechanics; Myosin V; Single molecule

Indexed keywords

MEROMYOSIN; MYOSIN; MYOSIN V; UNCLASSIFIED DRUG;

ACTIN FILAMENT; ANIMAL TISSUE; ARTICLE; BACULOVIRUS; CELL MOTILITY; CELL ORGANELLE; CELL TRANSPORT; MOLECULAR INTERACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL;

ANIMALIA; UNIDENTIFIED BACULOVIRUS;

EID: 0035969240     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (53)

1. Myosin motors with artificial lever arms
2. A large and distinct rotation of the myosin light chain domain occurs upon muscle contraction
3. Myosins: Matching functions with motors
4. The kinetic mechanism of myosin V
5. ADP inhibition of myosin V ATPase activity
6. Actin and light chain isoform dependence of myosin V kinetics
7. Actin filament mechanics in the laser trap
8. The light chain composition of chicken brain myosin-Va: Calmodulin, myosin-II essential light chains, and 8-kDa dynein light chain/PIN
9. Single myosin molecule mechanics: Piconewton forces and nanometer steps
10. Smooth muscle and skeletal muscle myosins produce similar unitary forces and displacements in the laser trap
11. Smooth and skeletal actin are mechanically indistinguishable in the in vitro motility assay
12. Smooth and skeletal muscle myosin both exhibit low duty cycles at zero load in vitro
13. Actin and myosin function in directed vacuole movement during cell division in Saccharomyces cerevisiae
14. A myosin family tree
15. Factors affecting movement of F-actin filaments propelled by skeletal muscle heavy meromyosin
16. Inhibition of smooth muscle myosin's activity and assembly by an anti-rod monoclonal antibody
17. Crossbridge behavior during muscle contraction
18. Conformation of the myosin motor during force generation in skeletal muscle
19. Dilute (d) coat colour mutation of DNA/2J mice is associated with the site of integration of an ecotropic MuLV genome
20. The Saccharomyces cerevisiae MYO2 gene encodes an essential myosin for vectorial transport of vesicles
21. A 7-amino acid insert in the heavy chain nucleotide binding loop alters the kinetics of smooth muscle myosin in the laser trap
22. Kinetic mechanism of myofibril ATPase
23. Myosin learns to walk
24. Detection of single-molecule interactions using correlated thermal diffusion
25. Myosin-V is a processive actin-based motor
26. Novel myosin heavy chain encoded by murine dilute coat colour locus
27. Unconventional myosins in cell movement, membrane traffic, and signal transduction
28. Characterization of myosin V binding to brain vesicles
29. Movement and force produced by a single myosin head
30. Kinetic differences at the single molecule level account for the functional diversity of rabbit cardiac myosin isoforms
31. Purification of muscle actin
32. Griscelli disease maps to chromosome 15q21 and is associated with mutations in the myosin-Va gene
33. Measuring kinetics of complex single ion channel data using mean-variance histograms
34. Cultured melanocytes from dilute mutant mice exhibit dendritic morphology and altered melanosome distribution
35. Structure of the actin-myosin complex and its implications for muscle contraction
36. Myosin-V stepping kinetics: A molecular model for processivity
37. Single-molecule tracking of myosins with genetically engineered amplifier domains
38. Direct observation of processive movement by individual myosin V molecules
39. The mechanism of force generation in myosin: A disorder-toorder transition, coupled to internal structural changes
40. Spare the rod, spoil the regulation: Necessity for a myosin rod
41. Monoclonal antibodies detect and stabilize conformational states of smooth muscle myosin
42. Kinetic characterization of a monomeric unconventional myosin V construct
43. Two heads of myosin are better than one for generating force and motion
44. Myosin step size. Estimation from slow sliding movement of actin over low densities of heavy meromyosin
45. The neck region of the myosin motor domain behaves as a lever arm to generate movement
46. Two-headed binding of a processive myosin to F-actin
47. Effect of ADP and ionic strength on the kinetic and motile properties of recombinant mouse myosin V
48. Smooth muscle myosin cross-bridge interactions modulate actin filament sliding velocity in vitro
49. Myosin conformational states determined by single fluorophore polarization
50. The light chain binding domain of expressed smooth muscle heavy meromyosin acts as a mechanical lever
51. Kinetics of nucleoside triphosphate cleavage and phosphate release steps by associated rabbit skeletal actomyosin, measured using a novel fluorescent probe for phosphate
52. Computer-assisted tracking of actin filament motility