Crystal structure of the motor domain of a class-I myosin

EMBO Journal

Volumn 21, Issue 11, 2002, Pages 2517-2525

  Kollmar, Martin ^a   Dürrwang, Ulrike ^a   Kliche, Werner ^a   Manstein, Dietmar J ^a   Kull, F Jon ^a  

^a Lamont Doherty Earth Observatory   (United States)

Author keywords

Crystal structure; Dictyostelium discoideum; Motor protein; Myosin I; Unconventional myosin

Indexed keywords

ACTIN; MOLECULAR MOTOR; MYOSIN;

ARTICLE; CELL MOTILITY; CRYSTAL STRUCTURE; DICTYOSTELIUM DISCOIDEUM; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN STABILITY;

ACTINS; ANIMALS; BINDING SITES; CHICKENS; CLONING, MOLECULAR; CRYSTALLOGRAPHY, X-RAY; DICTYOSTELIUM; KINETICS; MODELS, MOLECULAR; MUSCLE, SKELETAL; MYOSIN TYPE I; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; SRC HOMOLOGY DOMAINS;

DICTYOSTELIUM; DICTYOSTELIUM DISCOIDEUM;

EID: 0037013903     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/21.11.2517     Document Type: Article

References (62)

1. Binding of myosin I to membrane lipids
2. Myosin motors with artificial lever arms
3. Disruption of the gene encoding the p34/31 polypeptides affects growth and development of Dictyostelium discoideum
4. Kinetic analysis of Dictyostelium discoideum myosin motor domains with glycine-to-alanine mutations in the reactive thiol region
5. TEDS rule: A molecular rationale for differential regulation of myosins by phospharylation of the heavy chain head
6. A millennial myosin census
7. Crystallography and NMR system: A new software suite for macromolecular structure determination
8. Structural invariance of constitutively active and inactive mutants of Acanthamoeba myosin IC bound to F-actin in the rigor and ADP-bound states
9. Interaction of actin and ADP with the head domain of smooth muscle myosin: Implications for straindependent ADP release in smooth muscle
10. Kinetic mechanism and regulation of myosin VI
11. Crystal structure of a vertebrate smooth muscle myosin motor domain and its complex with the essential light chain: Visualization of the pre-power stroke state
12. 4
13. Modulation of actin affinity and actomyosin adenosin triphosphatase by charge changes in the myosin motor domain
14. Stabilization of the actomyosin complex by negative charges on myosin
15. A molecular basis for familial hypertrophic cardiomyopathy: A beta cardiac myosin heavy chain gene missense mutation
16. Myosin active-site trapping with vanadate ion
17. A myosin family tree
18. Atomic model of the actin filament
19. Atomic structure of scallop myosin subfragment SI complexed with MgADP: A novel conformation of the myosin head
20. Two conserved lysines at the 50/20 kDa junction of myosin are necessary for triggering actin-activation
21. Improved methods for binding protein models in electron density maps and the location of errors in these models
22. Brush border myosin-I structure and ADP-dependent conformational changes revealed by cryoelectron microscopy and image analysis
23. A 32° tail swing in brush border myosin I on ADP release
24. Three-dimensional structure of Acanthamoeba castellanii myosin-Ib (MIB) determined by cryoelectron microscopy of decorated actin filaments
25. Dictyostelium discoideum contains a gene encoding a myosin I heavy chain isoform
26. Sequence, expression pattern, extracellular localization, and targeted disruption of the Dictyostelium myosin ID heavy chain isoform
27. Dictyostelium mutants lacking multiple callsi myosin I isoforms reveal combinations of shared and distinct functions
28. Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants
29. Structure of a genetically engineered molecular motor
30. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
31. Loop I can modulate ADP affinity, ATPase activity, and motility of different scallop myosins. Transient kinetic analysis of SI isoforms
32. PROCHECK: A program to check the stereochemistry quality of protein structures
33. Myosin I mutants with only 1% of wild-type actin-activated MgATPase activity retain essential in vivo function(s)
34. An atomic model of the unregulated thin filament obtained by X-ray fiber diffraction on oriented actin-tropomyosin gels
35. Overexpression of myosin motor domains in Dictyostelium: Screening of transformants and purification of the affinity tagged protein
36. Cloning vectors for the production of proteins in Dictyostelium discoideum
37. Raster3D: Photorealistic molecular graphics
38. Protein-protein interactions in the rigor actomyosin complex
39. A myosin 11 mutation uncouples ATPase activity from motility and shortens step size
40. A myosin I is involved in membrane recycling from early endosomes
41. Dictyostelium myosin I double mutants exhibit conditional defects in pinocytosis
42. Molecular genetic analysis of myoC, a Dictyostelium myosin OI
43. Acanthamoeba myosin. I. Isolation from Acanthamoeba castellanii of an enzyme similar to muscle myosin
44. Three-dimensional structure of myosin subfragment-I: A molecular motor
45. Dictyostelium myosin IK is involved in the maintenance of cortical tension and affects motility and phagocytosis
46. The myosin relay helix to converter interface remains intact throughout the actomyosin ATPase cycle
47. X-ray structure of the magnesium(II)·ADP·vanadate complex of the Dictyostelium discoideum myosin motor domain to 1.9 Å resolution
48. How molecular motors work
49. Kinetic tuning of myosin via a flexible loop adjacent to the nucleotide binding pocket
50. Motor domain-dependent localization of myoIb (myr-I)
51. Examination of the endosomal and lysosomal pathways in Dictyostelium discoideum myosin I mutants
52. Multiple actin-based motor genes in Dictyostelium
53. Molecular genetic analysis of myoF, a new Dictyosteluim unconventional myosin gene
54. The Dictyostelium myosin IE heavy chain gene encodes a truncated isoform that lacks sequences corresponding to the actin binding site in the tail
55. The myosins in Dictyostelium
56. Differences in the ionic interaction of actin with the motor domains of nonmuscle and muscle myosin II
57. Functional characterization of the secondary actin binding site of myosin II
58. Evidence for cleft closure in actomyosin upon ADP release
59. Analysis of the regulatory phosphorylation site in Acanthamoeba myosin IC by using site-directed mutagenesis
60. Intrinsic tryptophan fluorescence identifies specific conformational changes at the actomyosin interface upon actin binding and ADP release
61. Is myosin a 'back door' enzyme?