Functional expression of chitinase and chitosanase, and their effects on morphologies in the yeast schizosaccharomyces pombe

Bioscience, Biotechnology and Biochemistry

Volumn 66, Issue 5, 2002, Pages 1143-1147

  Shimono, Kumiko ^a   Matsuda, Hideyuki ^a   Kawamukai, Makoto ^a  

^a SHIMANE UNIVERSITY   (Japan)

Author keywords

Chitinase; Chitosanase; Enterobacter sp. G 1; Matsuebacter chitosanotabidus; Schizosaccharomyces pombe

Indexed keywords

CYTOMEGALOVIRUS; ENTEROBACTER; ENTEROBACTER SP.; HUMAN HERPESVIRUS 5; MATSUEBACTER CHITOSANOTABIDUS; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE;

CHITINASE; CHITOSANASE; GLYCOSIDASE;

ARTICLE; CYTOLOGY; ENZYMOLOGY; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PLASMID; SCHIZOSACCHAROMYCES; WESTERN BLOTTING;

BLOTTING, WESTERN; CHITINASE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GLYCOSIDE HYDROLASES; PLASMIDS; SCHIZOSACCHAROMYCES;

EID: 0036559807     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.66.1143     Document Type: Article

References (17)

1. Lipke, P. N. and Ovalle, R., Cell wall architecture in yeast: new structure and new challenges. J. Bac-teriol., 180, 3735-3740 (1998).
2. Valdivieso, M. H., Duran, A., and Roncero, C., Chitin synthases in yeast and fungi. in “Chitin and Chitosan”, eds. Jolles, P., and Muzzarelli, R. A. A., Birkhauser Verlag, Switzerland, pp. 55-69 (1999).
3. Sietsma, J. H. and Wessels, J. G. H., The occurrence of glucosaminoglycan in the wall of Schizosaccharomyces pombe. J. Gener. Microbiol., 136, 2261-2265 (1990).
4. + chitin synthase gene in Schizosaccharomyces pombe. Mol. Microbiol., 35, 79-89 (2000).
5. Forsburg, S. L. and Sherman, D. A., General purpose tagging vectors for fission yeast. Gene, 191, 191-195 (1997).
6. Okada, H., Sekiya, T., Yokoyama, K., Tohda, H., Kumagai, H., and Morikawa, Y., Efficient secretion of Trichoderma reesei cellobiohydrolase II in Schizosaccharomyces pombe and characterization of its products. Appl. Microbiol. Biotechnol., 49, 301-308 (1998).
7. Okada, H., Tada, K., Sekiya, T., Yokoyama, K., Takahashi, A., Tohda, H., Kumagai, H., and Morikawa, Y., Molecular characterization and heterologous expression of the gene encoding a low-molecular-mass endoglucanase from Trichoderma reesei QM9414. Appl. Environ. Microbiol., 64, 555-563 (1998).
8. Tokunaga, M., Kawamura, A., Yonekyu, S., Kishida, M., and Hishinuma, F., Secretion of mouse a-amylase from fission yeast Schizosaccharomyces pombe: presence of chymostatin-sensitive protease activity in the culture medium. Yeast, 9, 379-387 (1993).
9. Draborg, H., Kauppinen, S., Dalbmge, H., and Christgau, S., Molecular cloning and expression in S. cerevisiae of two exochitinases from Trichoderma harzianum. Biochem. Mol. Biol. Int., 36, 781-791 (1995).
10. Draborg, H., Christgau, S., Halkier, T., Rasmussen, G., Dalbøge, H., and Kauppinen, S., Secretion of an enzymatically active Trichoderma harzianum en-dochitinase by Saccharomyces cerevisiae. Curr. Genet., 29, 404-409 (1996).
11. Park, J. K., Morita, K., Fukumoto, I., Yamasaki, Y., Nakagawa, T., Kawamukai, M., and Matsuda, H., Purification and characterization of the chitinase (ChiA) from Enterobacter sp. G-1. Biosci. Biotechnol. Biochem., 61, 684-689 (1997).
12. Park, J. K., Okamoto, T., Yamasaki, Y., Tanaka, K., Nakagawa, T., Kawamukai, M., and Matsuda, H., Molecular cloning, nucleotide sequencing, and regulation of the chiA gene encoding one of chitinases from Enterobacter sp. G-1. J. Ferment. Bioengin., 84, 493-501 (1997).
13. Park, J. K., Shimono, K., Ochiai, N., Shigeru, K., Kurita, M., Ohta, Y., Tanaka, K., Matsuda, H., and Kawamukai, M., Purification, characterization, and gene analysis of a chitosanase (ChoA) from Matsue-bacter chitosanotabidus 3001. J. Bacteriol., 181, 6642-6649 (1999).
14. Guthrie, C. and Fink, G. R., Guide to yeast genetics and molecular biology. Meth. Enzymol., 194, Academic press, New York (1991).
15. Basi, G., Schmid, E., and Maundrell, K., TATA box mutations in the Schizosaccharomyces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility. Gene, 123, 131-136 (1993).
16. Shimono, K., Shigeru, K., Tsuchiya, A., Itou, N., Ohta, Y., Tanaka, K., Nakagawal, T., Matsuda, H., and Kawamukai, M., Two glutamic acids in chitosanase A from Matsuebacter chitosanotabidus 3001 are the catalytically important residues. J. Biochem., 131, 87-96 (2002).
17. Imoto, T. and Yagishita, K., A simple activity measurement of lysozyme. Agri. Biol. Chem., 35, 1154-1156 (1971).