Specific detection of cytokeratin 20-positive cells in blood of colorectal and breast cancer patients by a high sensitivity real-time reverse transcriptase-polymerase chain reaction method

Journal of Molecular Diagnostics

Volumn 8, Issue 1, 2006, Pages 105-112

  Giribaldi, Giuliana ^a   Procida, Simone ^a   Ulliers, Daniela ^a   Mannu, Franca ^b   Volpatto, Roberta ^c   Mandili, Giorgia ^a   Fanchini, Laura ^c   Bertetto, Oscar ^c   Fronda, Gianruggero ^d   Simula, Luigi ^e   Rimini, Elena ^e   Cherchi, Giovanni ^e   Bonello, Lisa ^f   Maule, Milena Maria ^f   Turrini, Francesco ^a  

^a UNIVERSITY OF TURIN   (Italy)

^b Nurex Srl   (Italy)

^c Centro Oncologico ed Ematologico Subalpino (COES)   (Italy)

^d MOLINETTE HOSPITAL   (Italy)

^e Ospedale Santissima Annunziata   (Italy)

^f Center for Experimental Research and Medical Studies CERMS   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOKERATIN 20;

ADULT; ANALYTIC METHOD; ARTICLE; BLOOD CELL; BREAST CANCER; CANCER CELL CULTURE; CANCER PATIENT; COLORECTAL CANCER; CONTROLLED STUDY; DILUTION; EXON; FEMALE; GENE AMPLIFICATION; HUMAN; HUMAN CELL; LIVING DONOR; MAJOR CLINICAL STUDY; MALE; METASTASIS; NUCLEOTIDE SEQUENCE; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STATISTICAL SIGNIFICANCE;

EID: 32944477385     PISSN: 15251578     EISSN: None     Source Type: Journal    
DOI: 10.2353/jmoldx.2006.050054     Document Type: Article

References (47)

1. Bostick PJ, Chatterjee S, Chi DD, Huynh KT, Giuliano AE, Cote R, Hoon DS: Limitations of specific reverse-transcriptase polymerase chain reaction markers in the detection of metastases in the lymph nodes and blood of breast cancer patients. J Clin Oncol 1998, 16:2632-2640
2. Champelovier P, Mongelard F, Seigneurin D: CK20 gene expression: technical limits for the detection of circulating tumor cells. Anticancer Res 1999, 19:2073-2078
3. Hu XC, Chow LW: Fine needle aspiration may shed breast cells into peripheral blood as determined by RT-PCR. Oncology 2000, 59:217-222
4. Bae JW, Choi HK, Kim HG, Park SH: The detection of circulating breast cancer cells in peripheral blood by reverse transcriptase-polymerase chain reaction. J Korean Med Sci 2000, 15:190-198
5. Soong R, Beyser K, Basten O, Kalbe A, Rueschoff J, Tabiti K: Quantitative reverse transcription-polymerase chain reaction detection of cytokeratin 20 in noncolorectal lymph nodes. Clin Cancer Res 2000, 7:3423-3429
6. Schuster R, Max N, Mann B, Heufelder K, Thilo F, Grone J, Rokos F, Buhr HJ, Thie E, Keilholz U: Quantitative real-time RT-PCR for detection of disseminated tumor cells in peripheral blood of patients with colorectal cancer using different mRNA markers. Int J Cancer 2004, 108:219-227
7. Wyld DK, Selby P, Perren TJ, Jonas SK, Allen-Mersh TG, Wheeldon J, Burchill SA: Detection of colorectal cancer cells in peripheral blood by reverse-transcriptase polymerase chain reaction for cytokeratin 20. Int J Cancer 1998, 79:288-293
8. Moll R, Lowe A, Laufer J, Franke WW: Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies. Am J Pathol 1992, 140:427-447
9. Burchill SA, Bradbury MF, Pittman K, Southgate J, Smith B, Selby P: Detection of epithelial cancer cells in peripheral blood by reverse transcriptase-polymerase chain reaction. Br J Cancer 1995, 71:278-281
10. August DA, Ottow RT, Sugarbaker PH: Clinical perspective of human colorectal cancer metastasis. Cancer Metastasis Rev 1984, 3:303-302
11. Hughes KS, Simon R, Songhorabodi S, Adson MA, Ilstrup DM, Fortner JG, Maclean BJ, Foster JH, Daly JM, Fitzherbert D: Resection of the liver for colorectal carcinoma metastases: a multi-institutional study of patterns of recurrence. Surgery 1996, 100:278-284
12. Kienle P, Koch M, Autschbach F, Benner A, Treiber M, Wannenmacher M, von Knebel Doeberitz M, Buchler M, Herfarth C, Weitz J: Decreased detection rate of disseminated tumor cells of rectal cancer patients after preoperative chemoradiation: a first step towards a molecular surrogate marker for neoadjuvant treatment in colorectal cancer. Ann Surg 2003, 238:324-330
13. Weitz J, Koch M, Kienle P, Schrodel A, Willeke F, Benner A, Lehnert T, Herfarth C, von Knebel Doeberitz M: Detection of hematogenic tumor cell dissemination in patients undergoing resection of liver metastases of colorectal cancer. Ann Surg 2000, 232:66-72
14. Weitz J, Kienle P, Lacroix J, Willeke F, Benner A, Lehnert T, Herfarth C, von Knebel Doeberitz M: Dissemination of tumor cells in patients undergoing surgery for colorectal cancer. Clin Cancer Res 1998, 4:343-348
15. Guller U, Zajac P, Schnider A, Bosch B, Vorburger S, Zuber M, Spagnoli GC, Oertli D, Maurer R, Metzger U, Harder F, Heberer M, Marti WR: Disseminated single tumor cells as detected by real-time quantitative polymerase chain reaction represent a prognostic factor in patients undergoing surgery for colorectal cancer. Ann Surg 2002, 236:768-776
16. Hardingham JE, Hewett PJ, Sage RE, Finch JL, Nuttall JD, Kotasek D, Dobrovic A: Molecular detection of blood-borne epithelial cells in colorectal cancer patients and in patients with benign bowel disease. Int J Cancer 2000, 89:8-13
17. Stathopoulou A, Vlachonikolis I, Mavroudis D, Perraki M, Kouroussis Ch, Apostolaki S, Malamos N, Kakolyris S, Kotsakis A, Xenidis N, Reppa D, Georgoulias V: Molecular detection of cytokeratin-19-positive cells in the peripheral blood of patients with operable breast cancer: evaluation of their prognostic significance. J Clin Oncol 2002, 20:3404-3412
18. Liefers GJ, Cleton-Jansen AM, van de Velde CJ, Hermans J, van Krieken JH, Cornelisse CJ, Tollenaar RA: Micrometastases and survival in stage II colorectal cancer. N Engl J Med 1998, 339:372-379
19. Stosiek P, Kasper M, Karsten U, Goertchen R: Detection of cancer metastases in regional lymph nodes: comparative histological and immunohistological investigations with the broad-range anticytokeratin monoclonal antibody A45-B/B3. Neoplasma 1991, 38:43-47
20. Bosman FT: Molecular pathology of colorectal cancer. Cytogenet Cell Genet 1999, 86:112-117
21. Lassmann S, Bauer M, Rosenberg R, Nekarda H, Soong R, Ruger R, Hofler H, Werner M: Identification of occult tumor cells in node negative lymph nodes of colorectal cancer patients by cytokeratin 20 gene and protein expression. Int J Colorectal Dis 2004, 19:87-94
22. Bustin SA, Siddiqi S, Ahmed S, Hands R, Dorudi S: Quantification of cytokeratin 20, carcinoembryonic antigen and guanylyl cyclase C mRNA levels in lymph nodes may not predict treatment failure in colorectal cancer patients. Int J Cancer 2004, 108:412-417
23. Fisher B, Bauer M, Wickerham DL, Redmond CK, Fisher ER, Cruz AB, Foster R, Gardner B, Lerner H, Margolese R: Relation of number of positive axillary nodes to the prognosis of patients with primary breast cancer: an NSABP update. Cancer 1983, 52:1551-1557
24. Goldberg RM, Fleming TR, Tangen CM, Moertel CG, Macdonald JS, Haller DG, Laurie JA: Surgery for recurrent colon cancer: strategies for identifying resectable recurrence and success rates after resection: Eastern Cooperative Oncology Group, the North Central Cancer Treatment Group, and the Southwest Oncology Group. Ann Intern Med 1998, 129:27-35
25. Gilbey AM, Burnett D, Coleman RE, Holen I: The detection of circulating breast cancer cells in blood [Review]. J Clin Pathol 2004, 57:903-911
26. Miura M, Ichikawa Y, Tanaka K, Kamiyama M, Hamaguchi Y, Ishikawa T, Yamaguchi S, Togo S, Ike H, Ooki S, Shimada H: Real-time PCR (TaqMan PCR) quantification of carcinoembryonic antigen (CEA) mRNA In the peripheral blood of colorectal cancer patients. Anticancer Res 2003, 23:1271-1276
27. Hampton R, Walker M, Marshall J, Juhl H: Differential expression of carcinoembryonic antigen (CEA) splice variants in whole blood of colon cancer patients and healthy volunteers: implication for the detection of circulating colon cancer cells. Oncogene 2002, 21:7817-7823
28. Weigelt B, Bosma AJ, Hart AA, Rodenhuis S, van't Veer LJ: Marker genes for circulating tumour cells predict survival in metastasized breast cancer patients. Br J Cancer 2003, 88:1091-1094
29. Aerts J, Wynendaele W, Paridaens R, Christiaens MR, van den Bogaert W, van Oosterom AT, Vandekerckhove F: A real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) to detect breast carcinoma cells in peripheral blood. Ann Oncol 2001, 12:39-46
30. Stathopoulou A, Gizi A, Perraki M, Apostolaki S, Malamos N, Mavroudis D, Georgoulias V, Lianidou ES: Real-time quantification of CK-19 mRNA-positive cells in peripheral blood of breast cancer patients using the lightcycler system. Clin Cancer Res 2003, 9:5145-5151
31. Ko Y, Klinz M, Totzke G, Gouni-Berthold I, Sachinidis A, Vetter H: Limitations of the reverse transcription-polymerase chain reaction method for the detection of carcinoembryonic antigen-positive tumor cells in peripheral blood. Clin Cancer Res 1998, 4:2141-2146
32. Dimmler A, Gerhards R, Betz C, Gunther K, Reingruber B, Horbach T, Baumann I, Kirchner T, Hohenberger W, Papadopoulos T: Transcription of cytokeratins 8, 18, and 19 in bone marrow and limited expression of cytokeratins 7 and 20 by carcinoma cells: inherent limitations for RT-PCR in the detection of isolated tumor cells. Lab Invest 2001, 81:1351-1361
33. Vlems FA, Diepstra JH, Cornelissen IM, Ruers TJ, Ligtenberg MJ, Punt CJ, van Krieken JH, Wobbes T, van Muijen GN: Limitations of cytokeratin 20 RT-PCR to detect disseminated tumour cells in blood and bone marrow of patients with colorectal cancer: expression in controls and downregulation in tumour tissue. J Clin Pathol Mol Pathol 2002, 55:156-163
34. Zippelius A, Pantel K: RT-PCR-based detection of occult disseminated tumor cells in peripheral blood and bone marrow of patients with solid tumors: an overview [Review]. Ann NY Acad Sci 2000, 906:110-123
35. Bustin SA, Gyselman VG, Siddiqi S, Dorudi S: Cytokeratin 20 is not a tissue-specific marker for the detection of malignant epithelial cells in the blood of colorectal cancer patients. Int J Surg Investig 2000, 2:49-57
36. Chausovsky G, Luchansky M, Figer A, Shapira J, Gottfried M, Novis B, Bogelman G, Zemer R, Zimlichman, Klein A: Expression of cytokeratin 20 in the blood of patients with disseminated carcinoma of the pancreas, colon, stomach, and lung. Cancer 1999, 86:2398-2405
37. Vlems F, Soong R, Diepstra H, Punt C, Wobbes T, Tabiti K, van Muijen G: Effect of blood sample handling and reverse transcriptase-polymerase chain reaction assay sensitivity on detection of CK20 expression in healthy donor blood. Diagn Mol Pathol 2002, 11:90-97
38. Ikeguchi M, Ohro S, Maeda Y, Fukuda K, Yamaguchi K, Shirai H, Kondo A, Tsujitani S, Kaibara N: Detection of cancer cells in the peripheral blood of gastric cancer patients. Int J Mol Med 2003, 11:217-221
39. Zippelius A, Lutterbuse R, Riethmuller G, Pantel K: Analytical variables of reverse transcription-polymerase chain reaction-based detection of disseminated prostate cancer cells. Clin Cancer Res 2000, 6:2741-2750
40. Max N, Willhauck M, Wolf K, Thilo F, Reinhold U, Pawlita M, Thiel E, Keilholz U: Reliability of PCR-based detection of occult tumour cells: lessons from real-time RT-PCR. Melanoma Res 2001, 11:371-378
41. de Cremoux P, Extra JM, Denis MG, Pierga JY, Bourstyn E, Nos C, Clough KB, Boudou E, Martin EC, Muller A, Pouillart P, Magdelenat H: Detection of MUC1-expressing mammary carcinoma cells in the peripheral blood of breast cancer patients by real-time polymerase chain reaction. Clin Cancer Res 2000, 6:3117-3122
42. Park S, Lee B, Kim I, Choi I, Hong K, Ryu Y, Rhim J, Shin J, Park SC, Chung H, Chung J: Immunobead RT-PCR versus regular RT-PCR amplification of CEA mRNA in peripheral blood. J Cancer Res Clin Oncol 2001, 127:489-494
43. Ladanyi A, Soong R, Tabiti K, Molnar B, Tulassay Z: Quantitative reverse transcription-PCR comparison of tumor cell enrichment methods. Clin Chem 2001, 47:1860-1863
44. Max N, Wolf K, Thiel E, Keilholz U: Quantitative nested real-time RT-PCR specific for tyrosinase transcripts to quantitate minimal residual disease. Clin Chim Acta 2002, 317:39-46
45. Schamhart D, Swinnen J, Kurth KH, Westerhof A, Kusters R, Borchers H, Sternberg C: Numeric definition of the clinical performance of the nested reverse transcription-PCR for detection of hematogenous epithelial cells and correction for specific mRNA of non-target cell origin as evaluated for prostate cancer cells. Clin Chem 2003, 49:1458-1466
46. Cristofanilli M, Budd GT, Ellis MJ, Stopeck A, Matera J, Miller MC, Reuben JM, Doyle GV, Allard WJ, Terstappen LW, Hayes DF: Circulating tumor cells, disease progression, and survival in metastatic breast cancer. N Engl J Med 2004, 351:781-791
47. Franzen B, Linder S, Alaiya AA, Eriksson E, Uruy K, Hirano T, Okuzawa K, Auer G: Analysis of polypeptide expression in benign and malignant human breast lesions: down-regulation of cytokeratins. Br J Cancer 1996, 74:1632-1638