A real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) to detect breast carcinoma cells in peripheral blood

Annals of Oncology

Volumn 12, Issue 1, 2001, Pages 39-46

  Aerts, J ^a   Wynendaele, W ^a   Paridaens, R ^a   Christiaens, M R ^a   Bogaert, W V D ^a   Oosterom, A T V ^a   Vandekerckhove, F ^a  

^a UNIVERSITY HOSPITALS LEUVEN   (Belgium)

Author keywords

Breast cancer; Cytokeratin; Micrometastases; Peripheral blood; Quantitative PCR

Indexed keywords

CYTOKERATIN 19; MESSENGER RNA;

AGAR GEL ELECTROPHORESIS; ARTICLE; BLOOD; BONE MARROW METASTASIS; BREAST CANCER; CANCER CELL; CANCER RECURRENCE; CANCER STAGING; CARCINOMA CELL; CIRCULATION; CLINICAL ARTICLE; CLINICAL TRIAL; CONTAMINATION; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; FEMALE; GENETIC ANALYSIS; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; HUMAN TISSUE; LYMPH NODE METASTASIS; METASTASIS; NORTHERN BLOTTING; PILOT STUDY; POLYMERASE CHAIN REACTION; PREDICTION; PREOPERATIVE PERIOD; PRIORITY JOURNAL; QUANTITATIVE ASSAY; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SAMPLE; SENSITIVITY AND SPECIFICITY; TECHNIQUE; TIME; VOLUNTEER;

ADULT; BASE SEQUENCE; BREAST NEOPLASMS; FALSE POSITIVE REACTIONS; FEMALE; HUMANS; KERATINS; MOLECULAR SEQUENCE DATA; NEOPLASM CIRCULATING CELLS; NEOPLASM METASTASIS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING; TIME FACTORS; TRANSCRIPTION, GENETIC;

EID: 0035136684     PISSN: 09237534     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008317512253     Document Type: Article

References (29)

1. Adjuvant therapy for node negative breast cancer
2. Potential pathological application of immunocytochemical methods to the detection of micrometastases
3. Prediction of early relapse in patients with operable breast cancer by detection of occult bone marrow micrometastases
4. Detection of tumor cells in bone marrow of patients with primary breast cancer: A prognostic factor for distant metastasis
5. Tumour cell detection in the bone marrow of breast cancer patients at primary therapy: Results of a three-year median follow-up
6. Outcome of primary breast cancer patients with micrometastases: A long-term follow-up study
7. Meta-analyses of studies on bone marrow micrometastases: An independent prognostic impact remains to be substantiated
8. Cytokeratin-positive cells in the bone marrow and survival of patients with stage I, II, or III breast cancer
9. Detection of residual leukemia after bone marrow transplant for chronic myeloid leukemia: Role of polymerase chain reaction in predicting relapse
10. Keratin gene expression in non-epithelial tissues - Detection with polymerase chain reaction
11. Sensitive detection of occult breast cancer by the reverse-transcriptase polymerase chain reaction
12. Detection of epithelial cancer cells in peripheral blood by reverse-transcriptase polymerase chain reaction
13. Reverse transcriptase/polymerase chain reaction detection of cytokeratin-19 mRNA in bone marrow and blood of breast cancer patients
14. High-sensitivity detection of minimal residual breast carcinoma using the polymerase chain reaction and primers for cytokeratin 19
15. Comparison of molecular cytokeratin 19 reverse transcriptase polymerase chain reaction (CK19 RT-PCR) and immunocytochemical detection of micrometastatic breast cancer cells in hematopoietic harvests
16. Quantitative polymerase chain reaction for the detection of micrometastases in patients with breast cancer
17. Specific detection of carcinoembryonic antigen-expressing tumor cells in bone marrow aspirates by polymerase chain reaction
18. Clinical significance of molecular detection of carcinoma cells in lymph nodes and peripheral blood by reverse transcription-polymerase chain reaction in patients with gastrointestinal or breast carcinomas
19. Monitoring of tumor cell purging after highly efficient immunomagnetic selection of CD34 cells from leukapheresis products in breast cancer patients: Comparison of immunocytochemical tumor cell staining and transcriptase-polymerase chain reaction
20. Detection of cancer cells in peripheral blood of breast cancer patients using reverse transcription-polymerase chain reaction for epidermal growth factor receptor
21. Detection of metastatic breast cancer by β-hCG polymerase chain reaction
22. Sensitive detection of circulating breast cancer cells by reverse-transcriptase polymerase chain reation of maspin genes
23. Response of circulating tumor cells to systemic therapy in patients with metastatic breast cancer: Comparison of quantitative polymerase chain reaction and immunocytochemical techniques
24. Real-time quantitative PCR
25. Sequence of cDNA coding for human keratin 19
26. The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells
27. Limitations of specific reverse-transcriptase polymerase chain reaction markers in the detection of metastases in the lymph nodes and blood of breast cancer patients
28. Embryonic expression of the human 40-kd keration: Evidence from a processed pseudogene sequence
29. Identification of a novel cytokeratin 19 pseudogene that may interfere with reverse transcriptase-polymerase chain reaction assays used to detect micrometastatic tumor cells