Quantitative reverse transcription-PCR comparison of tumor cell enrichment methods

Clinical Chemistry

Volumn 47, Issue 10, 2001, Pages 1860-1863

  Ladanyi A ^a   Soong, R ^a   Tabiti, K ^a   Molnar B ^a   Tulassay, Z ^a  

^a Second Department of Medicine   (Hungary)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYTIC METHOD; ARTICLE; CELL DENSITY; CELL SEPARATION; COMPARATIVE STUDY; DENSITY GRADIENT; GENETIC MARKER; HUMAN; HUMAN TISSUE; NORMAL HUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TUMOR CELL;

EID: 0034834309     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/47.10.1860     Document Type: Article

References (18)

1. Molecular and immunological detection of circulating tumor cells and micrometastases from solid tumors
2. Molecular detection of micrometastases and circulating tumor cells in solid tumors
3. RT-PCR-based detection of occult disseminated tumor cells in peripheral blood and bone marrow of patients with solid tumors. An overview
4. Reliability of reverse transcription-polymerase chain reaction (RT-PCR)-based assays for the detection of circulating tumour cells: A quality-assurance initiative of the EORTC Melanoma Cooperative Group
5. Detection of micrometastasis by cytokeratin 20 RT-PCR is limited due to stable background transcription in granulocytes
6. Dissemination of tumor cells in patients undergoing surgery for colorectal cancer
7. Immunobead-PCR: A technique for the detection of circulating tumor cells using immunomagnetic beads and the polymerase chain reaction
8. Efficiency of Ber-EP4 antibody for isolating circulating epithelial cells before RT-PCR detection
9. Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies
10. Detection of epithelial cancer cells in peripheral blood by reverse transcriptase-polymerase chain reaction
11. The detection of disseminated tumour cells in bone marrow from colorectal cancer patients by a cytokeratin-20-specific nested reverse-transcriptase polymerase-chain reaction is related to the stage of disease
12. Cytokeratin 20 mRNA in peripheral blood of colorectal carcinoma patients
13. Quality management and influential factors for the detection of single metastatic cancer cells by reverse transcriptase polymerase chain reaction
14. Limitations of specific reverse-transcriptase polymerase chain reaction markers in the detection of metastases in the lymph nodes and blood of breast cancer patients
15. Detection of disseminated urothelial cancer cells in peripheral venous blood by a cytokeratin 20-specific nested reverse transcriptase-polymerase chain reaction
16. Expression of cytokeratin 20 in thyroid carcinomas and peripheral blood detected by reverse transcription polymerase chain reaction
17. CK20 gene expression: Technical limits for the detection of circulating tumor cells
18. Kinetic PCR analysis using a CCD camera and without using oligonucleotide probes