Real-Time Quantification of CK-19 mRNA-Positive Cells in Peripheral Blood of Breast Cancer Patients Using the Lightcycler System

Clinical Cancer Research

Volumn 9, Issue 14, 2003, Pages 5145-5151

  Stathopoulou, Aliki ^a,b   Gizi, Anna ^a   Perraki, Maria ^b   Apostolaki, Stella ^b   Malamos, Nikos ^c   Mavroudis, Dimitris ^b   Georgoulias, Vassilis ^b   Lianidou, Evi S ^a  

^a UNIVERSITY OF ATHENS   (Greece)

^b UNIVERSITY HOSPITAL OF HERAKLION   (Greece)

^c HELENA VENIZELOU HOSPITAL   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA;

ARTICLE; BREAST CANCER; CANCER DIAGNOSIS; CANCER PATIENT; CANCER STAGING; CELL HYBRIDIZATION; CELL STRAIN MCF 7; CONTROLLED STUDY; HUMAN; HUMAN CELL; MAJOR CLINICAL STUDY; METASTASIS; NUCLEOTIDE SEQUENCE; OCCULT CANCER; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ADOLESCENT; ADULT; AGED; ANTINEOPLASTIC AGENTS; BREAST NEOPLASMS; DNA PRIMERS; FEMALE; HUMANS; KERATINS; MIDDLE AGED; NEOPLASM CIRCULATING CELLS; NEOPLASM STAGING; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, NEOPLASM; SENSITIVITY AND SPECIFICITY; TUMOR CELLS, CULTURED;

EID: 0242525672     PISSN: 10780432     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Pantel, K., and Doeberitz, M. K. Detection and clinical relevance of micrometastatic cancer cells. Curr. Opin. Oncol., 12: 95-101, 2000.
2. Solakoglu, O., Maierhofer, C., Lahr, G., Breit, E., Scheunemann, P., Heumos, I., Pichlmeier, U., Schlimok, G., Oberneder, R., Kollermann, M. W., Kollermann, J., Speicher, M. R., and Pantel, K. Heterogeneous proliferative potential of occult metastatic cells in bone marrow of patients with solid epithelial tumors. Proc. Natl. Acad. Sci. USA, 99: 2246-2251, 2002.
3. Jiang, W. G., Martin, T. A., and Mansel, R. E. Molecular detection of micrometastasis in breast cancer (Review). Crit. Rev. Oncol./Hematol., 43: 13-31, 2002.
4. Klein, C. A., Blankenstein, T. F., Schmidt-Kittler, O., Petronio, M., Polzer, B., Stoecklein. N. H., and Riethmuller, G. Genetic heterogeneity of single disseminated tumour cells in minimal residual cancer. Lancet, 360: 683-689, 2002.
5. Muller, A., Homey, B., Soto, H., Ge, N., Catron, D., Buchanan, M. E., McClanahan, T., Murphy, E., Yuan, W., Wagner, S. N., Barrera, J. L., Mohar, A., Verastegui, E., and Zlotnik, A. Involvement of chemokine receptors in breast cancer metastasis. Nature (Lond.), 410: 50-56, 2001.
6. Singletary, S. E., Allred, C., Ashley, P., Bassett, L. W., Berry, D., Bland, K. I., Borgen, P. I., Clark, G., Edge, S. B., Hayes, D. F., Hughes, L. L., Hutter, R. V., Morrow. M., Page, D. L., Recht, A., Theriault, R. L., Thor, A., Weaver, D. L., Wieand, H. S., and Greene, F. L. Revision of the American Joint Committee on Cancer Staging System for Breast Cancer, J. Clin. Oncol., 20: 17, 3628-3636, 2002.
7. Braun, S., Pantel, K., Müller, P., Janni, W., Hepp, F., Kentenich, C. R., Gastroph, S., Wischnik, A., Dimpfl, T., Kindermann, G., Riethmuller, G., and Schlimok, G. Cytokeratin-positive cells in the bone marrow and survival of patients with stage I, II, or III breast cancer. N. Engl. J. Med., 342: 525-533, 2000.
8. Datta, Y. H., Adams, P. T., Drobyski, W. R., Ethier, S. P., Terry, V. H., and Roth, M. S. Sensitive detection of occult breast cancer by the reverse transcriptase-polymerase chain reaction. J. Clin. Oncol., 72: 475-482, 1994.
9. Schoenfeld, A., Kruger, K. H., Gomm, J., Sinnett, H. D., Gazet, J. C., Sacks, N., Bender, H. G., Luqmani, Y., and Coombes, R. C. The detection of micrometastasis in the peripheral blood and bone marrow of patients with breast cancer using immunohistochemistry and reverse transcriptase-polymerase chain reaction for keratin 19. Eur. J. Cancer, 33: 854-861, 1997.
10. Stathopoulou, A., Vlachonikolis, I., Mavroudis, D., Perraki, M., Kouroussis, Ch., Apostolaki, S., Malamos, N., Kakolyris, S., Kotsakis, A., Xenidis, N., Reppa, D., and Georgoulias, V. Molecular Detection of Cytokeratin-19 positive cells in the peripheral blood of patients with operable breast cancer: evaluation of their prognostic significance. J. Clin. Oncol., 20: 3404-3412, 2002.
11. Slade, M. J., Smith, B. M., Sinnett, H. D., Cross, N. C., and Coombes, R. C. Quantitative polymerase chain reaction for the detection of micrometastases in patients with breast cancer. J. Clin. Oncol., 77: 870-879, 1999.
12. Trummer, A., Kadar, J., Arseniev, L., Petersen, D., Ganser, A., and Lichtinghagen, R. Competitive cytokeratin 19 RT-PCR for quantification of breast cancer cells in blood cell suspensions. J. Hematother. Stem Cell Res., 9: 275-284. 2000.
13. Shammas, F. V., Van Eekelen, J. A., Wee, L., Heikkila, R., and Osland, A. Sensitive and quantitative one-step polymerase chain reaction using capillary electrophoresis and fluorescence detection for measuring cytokeratin 19 expression. Scand. J. Clin. Lab. Investig., 59: 635-642, 1999.
14. Aerts, J., Wynendaele, W., Paridaens, R., Christiaens, M. R., van den Bogaert, W., van Oosterom, A. T., and Vandekerckhove, F. A real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) to detect breast carcinoma cells in peripheral blood. Ann. Oncol., 12: 39-46, 2001.
15. Stathopoulou, A., Angelopoulou, K., Georgoulias, V., and Lianidou, E. S. Quantitative RT-PCR luminometric hybridization assay with an RNA-Internal standard for cytokeratin-19 mRNA in peripheral blood of patients with breast cancer. Clin. Biochem., 34: 651-659, 2001.
16. Bernard, P., and Wittner, C. T. Real time PCR technology for cancer diagnostics. Clin. Chem., 48: 1178-1185, 2002.
17. Bosma, A. J., Weigelt, B., Lambrechts, A. C., Verhagen, O. J., Pruntel, R., Hart, A., Rodenhuis, S., and van 't Veer, L. J. Detection of circulating breast tumor cells by differential expression of marker genes. Clin. Cancer Res., 8: 1871-1877, 2002.
18. Zehenter, B. K., Dillon, D. C., Jiang, Y., Xu, S., Benninghton, A., Molesh, D. A., Zhang, X., Reed, S. G., Persing, D. H., and Houghton, R. L. Application of a Multigene reverse transcription PCR assay for detection of Mammaglobin and complementary transcribed genes in breast cancer lymph nodes. Clin. Chem., 48: 1225-1231, 2002.
19. Wittwer, C. T., Ririe, K. M., Andrew, R. V., David, D. A., Gundry, R. A., and Balis, U. J. The Lightcycler: a microvolume multisample fluorimeter with rapid temperature control. Biotechniques, 22: 176-181, 1997.
20. J. C. Miller, and J. N. Miller. Statistics for Analytical Chemistry. New York: John Wiley & Sons, 1986.
21. Tricarico, C., Pinzani, P., Bianchi, S., Paglierani, M., Distante, V., Pazzagli, M., Bustin, S., and Orlando, C. Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. Anal. Biochem., 309: 293-300, 2002.