Nectarin IV, a potent endoglucanase inhibitor secreted into the nectar of ornamental tobacco plants. Isolation, cloning, and characterization

Plant Physiology

Volumn 139, Issue 3, 2005, Pages 1389-1400

  Naqvi, S M Saqlan ^a,c   Harper, April ^b   Carter, Clay ^a,d   Ren, Gang ^a   Guirgis, Adel ^a,e   York, William S ^b   Thornburg, Robert W ^a  

^a IOWA STATE UNIVERSITY   (United States)

^b UNIVERSITY OF GEORGIA   (United States)

^c PMAS ARID AGRICULTURE UNIVERSITY   (Pakistan)

^d UNIVERSITY OF MINNESOTA DULUTH   (United States)

^e MENOUFIA UNIVERSITY   (Egypt)

Author keywords

[No Author keywords available]

Indexed keywords

CLONING; PLANTS (BOTANY); PROTEINS; TOBACCO;

DOMAIN RESTRUCTURES; ENDOGLUCANASE INHIBITOR; INHIBITION ASSAYS; ORNAMENTAL TOBACCO PLANTS;

ENZYME INHIBITION;

CLONES; ENZYMES; PLANTS; TOBACCO;

BETA (ANGIOSPERM); LYCOPERSICON ESCULENTUM; MAGNOLIOPHYTA; NICOTIANA ALATA; NICOTIANA LANGSDORFFII; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; NICOTIANA X SANDERAE; SOLANUM TUBEROSUM; TRITICUM AESTIVUM;

CELLULASE; COMPLEMENTARY DNA; MESSENGER RNA; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; DRUG ANTAGONISM; GENE EXPRESSION REGULATION; GENETICS; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; PHYLOGENY; PROTEIN CONFORMATION; SECRETION; SEQUENCE ALIGNMENT; TOBACCO;

AMINO ACID SEQUENCE; CELLULASE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; DNA, COMPLEMENTARY; GENE EXPRESSION REGULATION, PLANT; GLYCOSYLATION; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PHYLOGENY; PLANT PROTEINS; PROTEIN CONFORMATION; RNA, MESSENGER; SEQUENCE ALIGNMENT; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TOBACCO;

EID: 32944472494     PISSN: 00320889     EISSN: None     Source Type: Journal    
DOI: 10.1104/pp.105.065227     Document Type: Article

References (66)

1. Ausubel FM, Brent R, Kingston RE, Moore DD, Smith JA, Seidman JG, Struhl K (1987) Current Protocols in Molecular Biology. John Wiley & Sons, New York
2. Baker HG, Baker I (1973) Some anthecological aspects of the evolution of nectar-producing flowers, particularly amino acid production in the nectar. In VH Heywood, ed, Taxonomy and Ecology. Proceedings of an International Symposium Held at the Department of Botany, University of Reading, Vol 5. Academic Press, London, pp 243-264
3. Baker HG, Baker I (1975) Studies of nectar-constitution and pollinator-plant coevolution. In LE Gilbert, PH Raven, eds, Coevolution of Animals and Plants. Symposium V, First International Congress of Systematic and Evolutionary Biology, Boulder, Colorado, August 1973. University of Texas Press, Austin, TX, pp 100-140
4. Baker HG, Baker I (1981) Chemical constituents of nectar in relation to pollination mechanisms and phylogeny. In MH Nitecki, ed, Biochemical Aspects of Evolutionary Biology. University of Chicago Press, Chicago, pp 131-171
5. Borderies G, Jamet E, Lafitte C, Rossignol M, Jauneau A, Boudart G, Monsarrat B, Esquerre-Tugaye MT, Boudet A, Pont-Lezica R (2003) Proteomics of loosely bound cell wall proteins of Arabidopsis thaliana cell suspension cultures: a critical analysis. Electrophoresis 24: 3421-3432
6. Boudart G, Jamet E, Rossignol M, Lafitte C, Borderies G, Jauneau A, Esquerre-Tugaye MT, Pont-Lezica R (2005) Cell wall proteins in apo-plastic fluids of Arabidopsis thaliana rosettes: identification by mass spectrometry and bioinformatics. Proteomics 5: 212-221
7. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein-dye binding. Anal Biochem 72: 248-254
8. Cabras P, Angioni A, Tuberoso C, floris I, Reniero F, Guillou C, Ghelli S (1999) Homogentisic acid: a phenolic acid as a marker of strawberry-tree (Arbutus unedo) honey. J Agric Food Chem 47: 4064-4067
9. Carpita NC, Gibeaut DM (1993) Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth. Plant J 3: 1-30
10. Carter C, Graham R, Thornburg RW (1999) Nectarin I is a novel, soluble germin-like protein expressed in the nectar of Nicotiana sp. Plant Mol Biol 41: 207-216
11. Carter C, Pan S, Zouhar J, Avila EL, Girke T, Raikhel NV (2004) The vegetative vacuole proteome of Arabidopsis thaliana reveals predicted and unexpected proteins. Plant Cell 16: 3285-3303
12. Carter C, Thornburg RW (2000) Tobacco Nectarin I: purification and characterization as a germin-like, manganese superoxide dismutase implicated in the defense of floral reproductive tissues. J Biol Chem 275: 36726-36733
13. Carter C, Thornburg RW (2003) The nectary-specific pattern of gene expression is regulated by multiple promoter elements in the tobacco Nectarin I promoter. Plant Mol Biol 51: 451-457
14. Carter C, Thornburg R (2004a) Tobacco Nectarin V is a flavin-containing berberine bridge enzyme-like protein with glucose oxidase activity. Plant Physiol 134: 460-469
15. Carter C, Thornburg RW (2004b) Is the nectar redox cycle a floral defense against microbial attack? Trends Plant Sci 9: 320-324
16. Carter C, Thornburg RW (2004c) Tobacco Nectarin III is a bifunctional enzyme with monodehydroascorbate reductase and carbonic anhydrase activities. Plant Mol Biol 54: 415-425
17. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156-159
18. Collins T, Gerday C, Feller G (2005) Xylanases, xylanase families and extremophilic xylanases. FEMS Microbiol Rev 29: 3-23
19. Coutinho PM, Henrissat B (1999) Carbohydrate-active enzymes: an integrated database approach. In HJ Gilbert, G Davies, B Henrissat, B Svensson, eds, Recent Advances in Carbohydrate Bioengineering. The Royal Society of Chemistry, Cambridge, pp 3-12
20. Deinzer ML, Thompson PA, Burgett DM, Isaacson DL (1977) Pyrrolizidine alkaloids: their occurrence in honey from tansy ragwort (Senecio jacobaea L.). Science 195: 497-499
21. Dixon M (1972) The graphical determination of Km and Ki. Biochem J 129: 197-202
22. Ecroyd CE, Franich RA, Kroese HW, Steward D (1995) Volatile constituents of Dactylanthus taylorii flower nectar in relation to flower pollination and browsing by animals. Phytochemistry 40: 1387-1389
23. Erlich HA, editor (1989) PCR Technology: Principles and Applications for DNA Amplification. Stockton Press, New York
24. Ferrari S, Vairo D, Ausubel FM, Cervone F, De Lorenzo G (2003) Tandemly duplicated Arabidopsis genes that encode polygalacturonase-inhibiting proteins are regulated coordinately by different signal transduction pathways in response to fungal infection. Plant Cell 15: 93-106
25. Ferreres F, Andrade P, Gil MI, Tomas Barberan FA (1996) Floral nectar phenolics as biochemical markers for the botanical origin of heather honey. Z Lebensm-Unters-Forsch 202: 40-44
26. Griebel C, Hess G (1940) The vitamin C content of flower nectar of certain Labiatae. Z Lebensm-Unters-Forsch 79: 168-171
27. Guex N, Peitsch MC (1997) SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling. Electrophoresis 18: 2714-2723
28. Halliwell B, Gutteridge J (1999) Free Radicals in Biology and Medicine. Oxford University Press, New York
29. Heinrich G (1989) Analysis of cations in nectars by means of a laser microprobe mass analyser (LAMMA). Beitr Biol Pflanz 64: 293-308
30. Kagawa H, Hirano H (1989) Sequence of a cDNA encoding soybean basic 7S globulin. Nucleic Acids Res 17: 8868
31. Kolivas S, Gayler KR (1993) Structure of the cDNA coding for conglutin gamma, a sulphur-rich protein from Lupinus angustifolius. Plant Mol Biol 21: 397-401
32. Kultunow AM, Truettner J, Cox KH, Walroth M, Goldberg RB (1990) Different temporal and spatial gene expression patterns occur during anther development. Plant Cell 2: 1201-1224
33. Kornaga T, Zyzak DV, Kintinar A, Baynes J, Thornburg R (1997) Genetic and biochemical characterization of a "lost" unstable flower color phenotype in interspecific crosses of Nicotiana sp. WWW J Biol 2: 8
34. Kwak JM, Kim SA, Hong SW, Nam HG (1997) Evaluation of 515 expressed sequence tags obtained from guard cells of Brassica campestris. Planta 202: 9-17
35. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
36. Lay F, Brugliera F, Anderson M (2003) Isolation and properties of floral defensins from ornamental tobacco and petunia. Plant Physiol 131: 1283-1293
37. Lever M (1972) New reaction for colorimetric determination of carbohydrates. Anal Biochem 47: 273-279
38. Lotan T, Ori N, Fluhr R (1989) Pathogenesis-related proteins are developmentally regulated in tobacco flowers. Plant Cell 1: 881-887
39. McGeoch DJ (1985) On the predictive recognition of signal peptide sequences. Virus Res 3: 271-286
40. Nakai K, Kanehisa M (1991) Expert system for predicting protein localization sites in Gram-negative bacteria. Proteins Struct Funct Genet 11: 95-110
41. Oberg KA, Ruysschaert JM, Azarkan M, Smolders N, Zerhouni S, Wintjens R, Amrani A, Looze Y (1998) Papaya glutamine cyclase, a plant enzyme highly resistant to proteolysis, adopts an all-beta conformation. Eur J Biochem 258: 214-222
42. Pauly M, Andersen LN, Kaupinen S, Kofod LV, York WS, Albersheim P, Darvill AG (1999) A xyloglucan-specific endo-β-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification, and characterization of the recombinant enzyme. Glycobiology 9: 93-100
43. Payan F, Leone P, Porciero S, Furniss C, Tahir T, Williamson G, Durand A, Manzanares P, Gilbert HJ, Juge N, et al (2004) The dual nature of the wheat xylanase protein inhibitor XIP-I: structural basis for the inhibition of family 10 and family 11 xylanases. J Biol Chem 279: 36029-36037
44. Peitsch MC (1995) Protein modeling by e-mail. Biotechnology 13: 658-660
45. Powell AL, van Kan J, ten Have A, Visser J, Greve LC, Bennett AB, Labavitch JM (2000) Transgenic expression of pear PGIP in tomato limits fungal colonization. Mol Plant Microbe Interact 13: 942-950
46. Qin Q, Bergmann CW, Rose JK, Saladie M, Kolli VS, Albersheim P, Darvill AG, York WS (2003) Characterization of a tomato protein that inhibits a xyloglucan-specific endoglucanase. Plant J 34: 327-338
47. Rodriguez-Arce AL, Diaz N (1992) The stability of beta-carotene in mango nectar. J Agric Univ PR 76: 101-102
48. Roshchina VV, Roshchina VD (1993) The Excretory Function of Higher Plants. Springer-Verlag, Berlin
49. Rowley D, Halliwell B (1983) Formation of hydroxyl radicals from hydrogen peroxide and iron salts by superoxide- and ascorbate-dependent mechanisms: relevance to the pathology of rheumatoid disease. Clin Sci (Lond) 64: 649-653
50. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406-425
51. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, Ed 2. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
52. Sansen S, De Ranter CJ, Gebruers K, Brijs K, Courtin CM, Delcour JA, Rabijns A (2004) Structural basis for inhibition of Aspergillus niger xylanase by Triticum aestivum xylanase inhibitor-I. J Biol Chem 279: 36022-36028
53. Satoh S, Sturm A, Fujii T, Chrispeels JJ (1992) cDNA cloning of an extracellular dermal glycoprotein of carrot and its expression in response to wounding. Planta 188: 432-438
54. Sayle RA, Milner-White EJ (1995) RASMOL: biomolecular graphics for all. Trends Biochem Sci 20: 374-376
55. Schenk PM, Kazan K, Wilson I, Anderson JP, Richmond T, Somerville SC, Manners JM (2000) Coordinated plant defense responses in Arabidopsis revealed by microarray analysis. Proc Natl Acad Sci USA 97: 11655-11660
56. Schilling S, Manhart S, Hoffmann T, Ludwig HH, Wasternack C, Demuth HU (2003) Substrate specificity of glutaminyl cyclases from plants and animals. Biol Chem 384: 1583-1592
57. Schwede T, Kopp J, Guex N, Peitsch MC (2003) SWISS-MODEL: an automated protein homology-modeling server. Nucleic Acids Res 31: 3381-3385
58. Segel IH (1975) Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady State Enzyme Systems. John Wiley & Sons, New York
59. Smith GP, Patel SU, Windass JD, Thornton JM, Winter G, Griffiths AD (1998) Small binding proteins selected from a combinatorial repertoire of knottins displayed on phage. J Mol Biol 277: 317-332
60. Thornburg RW, Carter C, Powell A, Rizhsky L, Mittler R, Horner HT (2003) A major function of the tobacco floral nectary is defense against microbial attack. Plant Syst Evol 238: 211-218
61. Vogel S (1969) Flowers offering fatty oil instead of nectar (abstract no. 229). In Abstracts of the Papers Presented at the XI International Botanical Congress, August 24-September 2, 1969 and the International Wood Chemistry Symposium, September 2-4, 1969. International Botanical Congress, Seattle
62. Wang Y, Sun J, Chitnis P (2000) Proteomic study of the peripheral proteins from thylakoid membranes of the cyanobacterium Synechocystis sp. PCC 6803. Electrophoresis 21: 1746-1754
63. Watanabe Y, Hirano H (1994) Nucleotide sequence of the basic 7S globulin gene from soybean. Plant Physiol 105: 1019-1020
64. Wei F, Wing RA, Wise RP (2002) Genome dynamics and evolution of the Mla (powdery mildew) resistance locus in barley. Plant Cell 14: 1903-1917
65. Wu S-C, Ham K-S, Darvill AG, Albersheim P (1997) Deletion of two endo-β-1,4-xylanase genes reveals additional isozymes secreted by the rice blast fungus. Mol Plant-Microbe Interact 10: 700-708
66. York WS, Qin Q, Rose JK (2004) Proteinaceous inhibitors of endo-beta-glucanases. Biochim Biophys Acta 1696: 223-233