Tobacco Nectarin V Is a Flavin-Containing Berberine Bridge Enzyme-Like Protein with Glucose Oxidase Activity

Plant Physiology

Volumn 134, Issue 1, 2004, Pages 460-469

  Carter, Clay J ^a,b   Thornburg, Robert W ^a  

^a IOWA STATE UNIVERSITY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; ELECTROPHORESIS; ENZYMES; MASS SPECTROMETRY; TOBACCO;

GEL ELECTROPHORESIS ANALYSIS;

PLANTS (BOTANY);

NICOTIANA ALATA; NICOTIANA LANGSDORFFII; NICOTIANA LANGSDORFII; NICOTIANA TABACUM;

EID: 0842264011     PISSN: 00320889     EISSN: None     Source Type: Journal    
DOI: 10.1104/pp.103.027482     Document Type: Article

References (36)

1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215: 403-410
2. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1992) Short Protocols in Molecular Biology. John Wiley & Sons, New York, p 917
3. Baker HG, Baker I (1973) Amino acids in nectar and their evolutionary significance. Nature 241: 543-545
4. Baker HG, Baker I (1975) Studies of nectar-constitution and pollinator-plant coevolution. In LE Gilbert, PH Raven, eds, PH Coevolution of Animals and Plants. University of Texas Press, Austin, pp 100-140
5. Baker HG, Baker I (1982) Floral nectar sugar constituents in relation to pollinator type. In CE Jones, RJ Little, eds, Handbook of Experimental Pollination Biology. Van Nostrand-Reinhold, New York, pp 107-115
6. Bang LM, Buntting C, Molan P (2003) The effect of dilution on the rate of hydrogen peroxide production in honey and its implications for wound healing. J Altem Complement Med 9: 267-273
7. Bird DA, Facchini PJ (2001) Berberine bridge enzyme, a key branch-point enzyme in benzylisoquinoline alkaloid biosynthesis, contains a vacuolar sorting determinant. Planta 213: 888-897
8. Carter C, Graham R, Thornburg RW (1999) Nectarin I is a novel, soluble germin-like protein expressed in the nectar of Nicotiana sp. Plant Mol Biol 41: 207-216
9. Carter C, Thornburg RW (2000) Tobacco Nectarin I: purification and characterization as a germin-like, manganese superoxide dismutase implicated in the defense of floral reproductive tissues. J Biol Chem 275: 36726-36733
10. Carter C, Thornburg RW (2003) The nectary-specific pattern of gene expression is regulated by multiple promoter elements in the tobacco Nectarin I promoter. Plant Mol Biol 51: 451-457
11. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156-159
12. Dittrich H, Kutchan TM (1991) Molecular cloning, expression, and induction of berberine bridge enzyme, an enzyme essential to the formation of benzophenanthridine alkaloids in the response of plants to pathogenic attack. Proc Natl Acad Sci USA 88: 9969-9973
13. Erlich HA (1989) PCR Technology: Principles and Applications for DNA Amplification. p 311
14. Facchini PJ, Penzes C, Hohnson AG, Bull D (1996) Moledular characterization of berbenne bridge enzyme gene from opium poppy. Plant Physiol 112: 1669-1677
15. Ge YX, Angenent GC, Wittich PE, Peters J, Franken J, Busscher M, Zhang LM, Dahlhaus E, Kater MM, Wullems GJ et al. (2000) NEC1, a novel gene, highly expressed in nectary tissue of Petunia hybrida. Plant J 24: 725-734
16. Griebel C, Hess G (1940) The vitamin C content of flower nectar of certain Labiatae. Zeit Untersuch Lebensmitt 79: 168-171
17. Hansen OC, Stougaard P (1997) Hexose oxidase from the red alga Chondrus crispus: purification, molecular cloning, and expression in Pichia pastoris. J Biol Chem 272: 11581-11587
18. Heinrich G (1989) Analysis of cations in nectars by means of a laser microprobe mass analyser (LAMMA). Beitr Biol Pflanz 64: 293-308
19. Ikezana N, Tanaka M, Nagayoshi M, Shinkyo R, Sakaki T, Inouye K, Sato F (2003) Molecular cloning and characterization of CYP719, a methylenedioxy bridge-forming enzyme that belongs to a novel p450 family, from cultured coptis japonicus cells. J Biol Chem 278: 38557-38565
20. Jaikaran ASI, Kennedy TD, Dratewka-Kos E, Lane BG (1990) Covalently bonded and adventitious glycans in germin. J Biol Chem 265: 12503-12512
21. Koltunow AM, Truettner, J, Cox KH, Wallroth M, Goldberg RB (1990) Different temporal and spatial gene expression patterns occur during anther development. Plant Cell 2: 1201-1224
22. Kornaga T, Zyzak DV, Kintinar A, Baynes J, Thornburg R (1997) Genetic and biochemical characterization of a "lost" unstable flower color phenotype in interspecific crosses of Nicotiana sp. WWW J Biol 2: 8
23. Kutchan TM, Dittrich H (1995) Characterization and mechanism of the berberine bridge enzyme, a covalently flavinylated oxidase of benzophenanthridine alkaloid biosynthesis in plants. J Biol Chem 270: 24475-24481
24. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
25. Lane BG, Dunwell JM, Ray JA, Schmitt MR, Cuming AC (1993) Germin, a protein marker of early plant development, is an oxalate oxidase. J Biol Chem 268: 12239-12242
26. Nakai K, Kanehisa M (1992) A knowledge base for predicting protein localization sites in eukaryotic cells. Genomics 14: 897-911
27. Ohmiya A, Tanaka Y, Kadowaki K, Hayashi T (1998) Cloning of genes encoding auxin-binding proteins (ABP19/20) from peach: significant peptide sequence similarity with germin-like proteins. Plant Cell Physiol 39: 492-499
28. Pazur JH, Kleppe K (1964) The oxidation of glucose and related compounds by glucose oxidase from Aspergillus niger. Biochemistry 35: 578-583
29. Rodriguez-Lopez M, Baroja-Fernandez E, Zandueta-Criado A, Moreno-Bruna B, Munoz FJ, Akazawa T, Pozueta-Romero J (2001) Two isoforms of a nucleotide-sugar pyrophosphatase/phosphodiesterase from barley leaves (Hordeum vulgare L.) are distinct oligomers of HvGLP1, a germin-like protein. FEBS Lett 490: 44-48
30. Sablowski R, Moyano E, Culianez-Macia F, Schuch W, Martin C, Bevan M (1994) A flower-specific Myb protein activates transcription of phenylpropanoid biosynthetic genes. EMBO J 13: 128-137
31. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
32. Segarra CI, Casalongue CA, Pinedo ML, Ronchi VP, Conde RD (2003) A germin-like protein of wheat leaf apoplast inhibits serine proteases. J Exp Bot 54: 1335-1341
33. Thornburg RW, Carter C, Powell A, Rizhsky L, Mittler R, Horner HT (2003) A major function of the tobacco floral nectary is defense against microbial attack. Plant Syst Evol 238: 211-218
34. Uimari A, Strommer J (1997) Myb26: a MYB-like protein of pea flowers with affinity for promoters of phenylpropanoid genes. Plant J 12: 1273-1284
35. Vogel S (1969) Flowers offering fatty oil instead of nectar. Abstracts of the XIth International Botanical Congress, Seattle, p 229
36. Wang Y, Sun J, Chitnis P (2000) Proteomic study of the peripheral proteins from thylakoid membranes of the cyanobacterium Synechocystis sp. PCC6803. Electrophoresis 21: 1746-1754