Expression of glycosylated haloalkane dehalogenase LinB in Pichia pastoris

Protein Expression and Purification

Volumn 46, Issue 1, 2006, Pages 85-91

  Nakamura, Takashi ^a   Zámocký, Marcel ^a,b   Zdráhal, Zbyněk ^a   Chaloupková, Radka ^a   Monincová, Marta ^a   Prokop, Zbyněk ^a   Nagata, Yuji ^c   Damborský, Jiří ^a  

^a MASARYK UNIVERSITY   (Czech Republic)

^b INSTITUTE OF CHEMISTRY   (Slovakia)

^c TOHOKU UNIVERSITY   (Japan)

Author keywords

Catalytic activity; Dehalogenation; Glycosylation; Heterologous expression; Pichia pastoris; Thermal stability

Indexed keywords

HALOALKANE DEHALOGENASE; HYDROLASE; RECOMBINANT PROTEIN;

ARTICLE; CHEMISTRY; CIRCULAR DICHROISM; ENZYME STABILITY; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; GLYCOSYLATION; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; PICHIA; PROTEIN CONFORMATION; PROTEIN DENATURATION; SPHINGOMONAS;

CIRCULAR DICHROISM; CLONING, MOLECULAR; ENZYME STABILITY; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GLYCOSYLATION; HYDROLASES; KINETICS; PICHIA; PROTEIN CONFORMATION; PROTEIN DENATURATION; RECOMBINANT PROTEINS; SPHINGOMONAS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; PICHIA PASTORIS; SPHINGOMONAS PAUCIMOBILIS;

EID: 32644476853     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.08.022     Document Type: Article

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