Sensitive monoclonal antibody-based sandwich ELISA for the detection of porcine skeletal muscle in meat and feed products

Journal of Food Science

Volumn 71, Issue 1, 2006, Pages

  Liu, Lihua ^a   Chen, Fur Chi ^b   Dorsey, Jodee L ^a   Hsieh, Yun Hwa Peggy ^a  

^a FLORIDA STATE UNIVERSITY   (United States)

^b TENNESSEE STATE UNIVERSITY   (United States)

Author keywords

ELISA; Feed; Meat and bone meal; Monoclonal antibody; Pork

Indexed keywords

MAMMALIA; SUIDAE;

EID: 32444445249     PISSN: 00221147     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2621.2006.tb12393.x     Document Type: Article

References (21)

1. Asero R, Mistrello G, Falagiani P. 1997. Oral allergy syndrome from pork. Allergy 52:684-6.
2. Ayob MK, Ragab AA, Allen JC, Farag RS, Smith CJ. 1989. An improved, rapid, ELISA technique for detection of pork in meat products. J Sci Food Agric 49:103-16.
3. Calvo JH, Osta R, Zaragoza P. 2002. Quantitative PCR detection of pork in raw and heated ground beef and pate. J Agric Food Chem 50(19):5265-7.
4. Calvo JH, Zaragoza P, Osta R. 2001. Technical note: a quick and more sensitive method to identify pork in processed and unprocessed food by PCR amplification of a new specific DNA fragment. J Anim Sci 79:2108-12.
5. Chen FC, Hsieh YHP. 2000. Detection of pork in heat-processed meat products by monoclonal antibody-based ELISA. J AOAC Int 83:79-85
6. Chen FC, Hsieh YHP 2002. Porcine troponin I: a thermostable species marker protein. Meat Sci 61:55-60.
7. Chen FC, Hsieh YHP, Bridgman RC. 1998. Monoclonal antibody to porcine thermal-stable muscle protein for detection of pork in raw and cooked meats. J Food Sci 63:201-5.
8. Chen FC, Hsieh YHP, Bridgman RC. 2002. Monoclonal antibodies against troponin I for the detection of rendered muscle tissues in animal feedstuffs. Meat Sci 62:405-12.
9. Ding HB, Xu RJ. 2000. Near-infrared spectroscopic technique for detection of beef hamburger adulteration. J Agric Food Chem. 48(6):2193-8.
10. European Commission. 2001. Commission Decision of 29 December 2000 concerning control measures required for the implementation of Council Decision 2000/766/EC concerning certain protection measures with regard to transmissible spongiform encephalopathies and the feeding of animal protein. Off J Eur Commun L002:32-40.
11. Harlow ED, Lane D. 1988. Antibodies: a laboratory manual. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press.
12. Hsieh YHP, Zhang S, Chen FC, Sheu SC. 2002. Monoclonal antibody based ELISA for assessment of endpoint heating temperature of ground pork and beef. J Food Sci 67(3):1149-54.
13. Kim H, Shelef LA. 1986. Characterization and identification of raw beef, pork, chicken, and turkey meats by electrophoretic patterns of their sarcoplasmic proteins. J Food Sci 51:731-5, 741.
14. Lahiff S, Glennon M, O'Brien L, Lying J, Smith T, Maher M, Shilton N. 2001. Species-specific PCR for the identification of ovine, porcine and chicken species in meat and bone meal (MBM). Mol Cell Probe 15:27-35.
15. Martin R, Azcona JI, Casas C, Hernandez PE, Sanz B. 1988. Sandwich ELISA for detection of pig meat in raw beef using antisera to muscle soluble proteins. J Food Protect 51:790-4.
16. Meyer R, Candrian U, Lüthy J. 1994. Detection of pork in heated meat products by the polymerase chain reaction. J Assoc Off Anal Chem 77(3):617-22.
17. Moncilovic D, Rasooly A. 2000. Detection and analysis of animal materials in food and feed. J Food Protect 63(11):1602-9.
18. Montiel-Sosa JF, Ruiz-Pesini E, Montiya J, Roncales P, Lopez-Perez MJ, Perez-Martos A. 2000. Direct and highly species-specific detection of pork meat and fat in meat products by PCR amplification of mitochondrial DNA. J Agric Food Chem 48(7):2829-32.
19. Morales P, Garcia T, Gonzalez I, Martin R, Sanz B, Hernandez PE. 1994. Monoclonal antibody detection of porcine meat. J Food Protect 57:146-9.
20. Saeed T, Ali SG, Abdul Rahman HA, Sawaya WN. 1989. Detection of pork and lard as adulterants in processed meat: Liquid chromatographic analysis of derivatized triglycerides. J Assoc Off Anal Chem 72:921-5.
21. Sawaya WN, Mameesh MS, El-Rayes E, Husain A, Dashti B. 1990. Detection of pork in processed meat by an enzyme-linked immunosorbent assay using antiswine antisera. J Food Sci 55:293-7.