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Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
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Materials and methods are available as supporting material on Science Online.
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note
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We thank J. Weissman, J. Newman, M. Noble, J. DeRisi, and M. Bigos for assistance with flow cytometry; A. Frankel, A. Ramirez, and M. Daugherty for assistance with peptide synthesis; D. King and E. Witkowska for assistance with mass spectrometry; and J. Weissman, D. Morgan, A. Bhatt, S. Collins, V. Denk, J. Dueber, and members of the Lim lab for helpful discussion. This work was supported by a postdoctoral fellowship from the Jane Coffin Childs Memorial Fund for Medical Research (A.R.), the University of California-San Francisco Medical Scientist Training Program and Burroughs Wellcome Fund Program in Quantitative Biology fellowship (R.P.B.), a University of California Graduate Research and Education in Adaptive Bio-Technology fellowship (C.J.B.), and grants from the NIH and Sandier Kirsch Foundations (W.A.L.). Coordinates have been deposited in the Protein Database (2F49, Fus3/Ste5_pep; 2FA2, Fus3 without Ste5_pep; 2F9G, Fus3-pY).
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