A molecular beacon-based real-time NASBA assay for detection of Mycobacterium avium subsp. paratuberculosis in water and milk

FEMS Microbiology Letters

Volumn 237, Issue 1, 2004, Pages 119-126

  Rodríguez Lázaro, David ^a   Lloyd, Joy ^b   Herrewegh, Arnold ^c   Ikonomopoulos, John ^d   D'Agostino, Martin ^b   Pla, Maria ^a   Cook, Nigel ^b  

^a UNIVERSITY OF GIRONA   (Spain)

^b CENTRAL SCIENCE LABORATORY   (United Kingdom)

^c NATIONAL INSTITUTE FOR PUBLIC HEALTH AND THE ENVIRONMENT   (Netherlands)

^d AGRICULTURAL UNIVERSITY OF ATHENS   (Greece)

Author keywords

Food products; Internal amplification control (IAC); Milk; Mycobacterium avium subsp. paratuberculosis; Real time NASBA

Indexed keywords

WATER;

ADDITION REACTION; ARTICLE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; CENTRIFUGATION; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; EXTRACTION; GENE AMPLIFICATION; GENE IDENTIFICATION; GENE TARGETING; MILK; MOLECULAR DYNAMICS; MYCOBACTERIUM AVIUM; MYCOBACTERIUM PARATUBERCULOSIS; NONHUMAN; NUCLEIC ACID ANALYSIS; PRIORITY JOURNAL;

ANIMALS; BACTERIAL PROTEINS; DNA, BACTERIAL; DNA-BINDING PROTEINS; FOOD MICROBIOLOGY; FRESH WATER; GENES, BACTERIAL; MILK; MYCOBACTERIUM PARATUBERCULOSIS; QUALITY CONTROL; SELF-SUSTAINED SEQUENCE REPLICATION; SENSITIVITY AND SPECIFICITY; WATER MICROBIOLOGY;

BACTERIA (MICROORGANISMS); MYCOBACTERIUM; MYCOBACTERIUM AVIUM; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS;

EID: 3242756691     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.femsle.2004.06.024     Document Type: Article

References (46)

1. Stabel J.R. Johne's disease: a hidden threat. J. Dairy Sci. 81:1998;283-288
2. Whitlock R.H., Buergelt C. Preclinical and clinical manifestations of paratuberculosis (including pathology). Vet. Clin. N. Am. Food Anim. Pract. 12:1996;345-356
3. McFadden J.J., Butcher P.D., Chiodini R., Hermon-Taylor J. Crohn's disease-isolated mycobacteria are identical to Mycobacterium paratuberculosis, as determined by DNA probes that distinguish between mycobacterial species. J. Clin. Microbiol. 25:1987;796-801
4. Chamberlin W., Graham D.Y., Hulten K., El-Zimaity H.M., Schwartz M.R., Naser S., Shafran I., El-Zaatari F.A. Review article: Mycobacterium avium subsp. paratuberculosis as one cause of Crohn's disease. Aliment. Pharmacol. Ther. 15:2001;337-346
5. Chiodini R.J. Crohn's disease and the mycobacterioses: a review and comparison of two disease entities. Clin. Microbiol. Rev. 2:1989;90-117
6. Collins M.T. Mycobacterium paratuberculosis: a potential food-borne pathogen. J. Dairy Sci. 80:1997;3445-3448
7. Covert T.C., Rodgers M.R., Reyes A.L., Stelma G.N. Jr. Occurrence of nontuberculous mycobacteria in environmental samples. Appl. Environ. Microbiol. 65:1999;2492-2496
8. Grant I., Rowe M.T., Dundee L., Hitchings E. Mycobacterium avium subsp. paratuberculosis: its incidence, heat resistance and detection in milk and dairy products. Int. J. Dairy Technol. 54:2001;2-13
9. Hermon-Taylor J., Bull T. Crohn's disease caused by Mycobacterium avium subspecies paratuberculosis: a public health tragedy whose resolution is long overdue. J. Med. Microbiol. 51:2002;3-6
10. Mishina D., Katsel P., Brown S.T., Gilberts E.C., Greenstein R.J. On the etiology of Crohn disease. Proc. Natl. Acad. Sci. USA. 93:1996;9816-9820
11. Rhan, K., Shin, S., Wilson, J., Johnson, R.P., Alves, D., McNab, B., Lachowski, W., Odumeru, J. and Spika, J. (1998) Milk as a potential source of human exposure to Mycobacterium avium subsp. paratuberculosis. In: Proceedings of 98th General Meeting of the American Society for Microbiology, Washington, D.C., p. 504
12. Taylor T.K., Wilks C.R., McQueen D.S. Isolation of Mycobacterium paratuberculosis from the milk of a cow with Johne's disease. Vet. Rec. 109:1981;532-533
13. Hance A.J., Grandchamp B., Levy-Frebault V., Lecossier D., Rauzier J., Bocart D., Giquel B. Detection and identification of mycobacteria by amplification of mycobacterial DNA. Mol. Microbiol. 3:1989;843-849
14. Compton J. Nucleic acid sequence-based amplification. Nature. 350:1991;91-92
15. Kievits T., van Gemen B., van Strijp D., Schukkink R., Dircks M., Adriaanse H., Malek L., Sooknanan R., Lens P. NASBA isothermal enzymatic in vitro nucleic acid amplification optimised for the diagnosis of HIV-1 infection. J. Virol. Meth. 35:1991;273-286
16. Chan A.B., Fox J.D. NASBA and other transcription-based amplification methods for research and diagnostic microbiology. Rev. Med. Microbiol. 10:1999;185-196
17. Uyttendaele M., Schukkink R., van Gemen B., Debevere J. Development of NASBA, a nucleic acid amplification system, for identification of Listeria monocytogenes and comparison to ELISA and a modified FDA method. Int. J. Food Microbiol. 27:1995;77-89
18. Uyttendaele M., Schukkink R., van Gemen B., Debevere J. Detection of Campylobacter jejuni added to foods by using a combined selective enrichment and nucleic acid sequence-based amplification (NASBA). Appl. Environ. Microbiol. 61:1995;1341-1437
19. van Gemen B., van Beuningen R., Nabbe A., van Strijp D., Jurriaans S., Lens P., Kievits T. A one-tube quantitative HIV-1 RNA NASBA nucleic acid amplification assay using electrochemiluminescent (ECL) labelled probes. J. Virol. Meth. 49:1994;157-167
20. Tyagi S., Kramer F.R. Molecular beacons: probes that fluoresce upon hybridisation. Nature Biotechnol. 14:1996;303-308
21. Leone G., van Schijndel H., van Gemen B., Kramer F.R., Schoen C.D. Molecular beacon probes combined with amplification by NASBA enable homogeneous, real-time detection of RNA. Nucl. Acids Res. 26:1998;2150-2155
22. Stryer L. Fluorescence energy transfer as a spectroscopic ruler. Annu. Rev. Biochem. 47:1978;819-846
23. Tyagi S., Bratu D.P., Kramer F.R. Multicolor molecular beacons for allele discrimination. Nature Biotechnol. 16:1998;49-53
24. Bull T.J., McMinn E.J., Sidi-Boumedine K., Skull A., Durkin D., Neild P., Rhodes G., Pickup R., Hermon-Taylor J. Detection and verification of Mycobacterium avium subsp. paratuberculosis in fresh ileocolonic mucosal biopsy specimens from individuals with and without Crohn's disease. J. Clin. Microbiol. 41:2003;2915-2923
25. Fang Y., Wu W.H., Pepper J.L., Larsen J.L., Marras S.A., Nelson E.A., Epperson W.B., Christopher-Hennings J. Comparison of real-time, quantitative PCR with molecular beacons to nested PCR and culture methods for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples. J. Clin. Microbiol. 40:2002;287-291
26. Motiwala A.S., Strother M., Amonsin A., Byrum B., Naser S.A., Stabel J.R., Shulaw W.P., Bannantine J.P., Kapur V., Sreevatsan S. Molecular epidemiology of Mycobacterium avium subsp. paratuberculosis: evidence for limited strain diversity, strain sharing, and identification of unique targets for diagnosis. J. Clin. Microbiol. 41:2003;2015-2026
27. O'Mahony J., Hill C. A real-time PCR assay for the detection and quantitation of Mycobacterium avium subsp. paratuberculosis using SYBR Green and the Light Cycler. J. Microbiol. Meth. 51:2002;283-293
28. Pillai S.R., Jayarao B.M. Application of IS900 PCR for detection of Mycobacterium avium subsp. paratuberculosis directly from raw milk. J. Dairy Sci. 85:2002;1052-1057
29. Hoorfar J., Malorny B., Abdulmawjood A., Cook N., Wagner M., Fach P. Practical considerations in design of internal amplification control for diagnostic PCR assays. J. Clin. Microbiol. 42:2004;1863-1868
30. Ballagi-Pordány A., Belák S. The use of mimics as internal standards to avoid false negatives in diagnostic PCR. Mol. Cell. Probes. 10:1996;159-164
31. Al-Soud W., Radstrom P. Purification and characterization of PCR-inhibitory components in blood cells. J. Clin. Microbiol. 39:2001;485-493
32. Rossen L., Norskov P., Holmstrom K., Rasmussen O.F. Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions. Int. J. Food Microbiol. 17:1992;37-45
33. Sambrook J., Russell D. Molecular Cloning: A Laboratory Manual. third ed. 2001;Cold Spring Harbour Laboratory, Cold Spring Harbour, NY
34. Simpkins S.A., Chan A.B., Hays J., Pöpping J., Cook N. An RNA transcription-based amplification technique (NASBA) for the detection of viable Salmonella enterica. Lett. Appl. Microbiol. 30:2000;75-79
35. Bannantine J.P., Baechler E., Zhang Q., Li L., Kapur V. Genome scale comparison of Mycobacterium avium subsp. paratuberculosis with Mycobacterium avium subsp. avium reveals potential diagnostic sequences. J. Clin. Microbiol. 40:2002;1303-1310
36. Rodríguez-Lázaro, D., D'Agostino, M., Pla, M. and Cook, N. (2004), Unpublished work
37. van Beckhoven J.R., Stead D.E., van der Wolf J.M. Detection of Clavibacter michiganensis subsp. sepedonicus by AmpliDet RNA, a new technology based on real time monitoring of NASBA amplicons with a molecular beacon. J. Appl. Microbiol. 93:2002;840-849
38. Lanciotti R.S., Kerst A.J. Nucleic acid sequence-based amplification assays for rapid detection of West Nile and St. Louis encephalitis viruses. J. Clin. Microbiol. 39:2001;4506-4513
39. Knutsson R., Blixt Y., Grage H., Borch E., Rådström P. Evaluation of selective enrichment PCR procedures for Yersinia enterocolitica. Int. J. Food Microbiol. 73:2002;35-46
40. Rodríguez-Lázaro, D., Lloyd, J., Ikonomopoulos, J., Pla, M. and Cook, N. (2004) Unexpected detection of DNA by nucleic acid sequence-based amplification technique. Mol. Cell. Probes., in press
41. Anonymous (2002) Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - General method specific requirements (EN ISO 22174). International Organization for Standardization, Geneva, Switzerland
42. Anonymous (2003) Microbiology of food and animal feeding stuffs - Protocol for the validation of alternative methods (EN ISO 16140). International Organization for Standardization, Geneva, Switzerland
43. Abdulmawjood A., Roth S., Bülte M. Two methods for construction of internal amplification controls for the detection of Escherichia coli O157 by polymerase chain reaction. Mol. Cell Probes. 16:2002;335-339
44. Cook N. The use of NASBA for the detection of microbial pathogens in food and environmental samples. J. Microbiol. Meth. 53:2003;165-174
45. Pillai S.R., Jayarao B.M. Application of IS900 PCR for detection of Mycobacterium avium subsp. paratuberculosis directly from raw milk. J. Dairy Sci. 85:2002;1052-1057
46. Khare S., Ficht T.A., Santos R.L., Romano J., Ficht A.R., Zhang S., Grant I.R., Libal M., Hunter D., Adams L.G. Rapid and sensitive detection of Mycobacterium avium subsp. paratuberculosis in bovine milk and feces by a combination of immunomagnetic bead separation-conventional PCR and real-time PCR. J. Clin. Microbiol. 42:2004;1075-1081