A molecular technique for detecting the liberation of intracellular zinc in cultured neurons

Journal of Neuroscience Methods

Volumn 137, Issue 2, 2004, Pages 175-180

  Hara, Hirokazu ^a   Aizenman, Elias ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

Cadmium; Cortical neurons; Luciferase; Metal response element; Metal response element binding transcription factor; Metallothionein; Transfection; Zinc

Indexed keywords

CADMIUM; LUCIFERASE; N METHYL DEXTRO ASPARTIC ACID; TRANSCRIPTION FACTOR; ZINC;

ANIMAL CELL; ARTICLE; BINDING SITE; BRAIN CELL; CELL CULTURE; CELL DEATH; CELL TYPE; CONTROLLED STUDY; MENTAL CAPACITY; MOLECULAR MECHANICS; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; REPORTER GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TECHNIQUE;

ANIMALS; CADMIUM; CELLS, CULTURED; CEREBRAL CORTEX; DNA-BINDING PROTEINS; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG INTERACTIONS; EMBRYO; ENZYME ACTIVATION; EXCITATORY AMINO ACID AGONISTS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENES, REPORTER; INTRACELLULAR SPACE; LUCIFERASES; MODELS, NEUROLOGICAL; MOLECULAR PROBE TECHNIQUES; N-METHYLASPARTATE; NEURONS; RATS; RATS, SPRAGUE-DAWLEY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TRANSCRIPTION FACTORS; VALINE; ZINC;

EID: 3242725995     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2004.02.018     Document Type: Article

References (29)

1. Aizenman E., Stout A.K., Hartnett K.A., Dineley K.E., McLaughlin B., Reynolds I.J. Induction of neuronal apoptosis by thiol oxidation: putative role of intracellular zinc release. J. Neurochem. 75:2000;1878-1888
2. Ascher P., Nowak L. The role of divalent cations in the N-methyl-D-aspartate responses of mouse central neurones in culture. J. Physiol. 399:1988;247-266
3. Bittel D., Dalton T., Samson S.L., Gedamu L., Andrews G.K. The DNA binding activity of metal response element-binding transcription factor-1 is activated in vivo and in vitro by zinc, but not by other transition metals. Biol. Chem. 273:1998;7127-7133
4. Chen X., Zhang B., Harmon P.M., Schaffner W., Peterson D.O., Giedroc D.P. A Novel cysteine cluster in human metal-responsive transcription factor 1 is required for heavy metal-induced transcriptional activation in vivo. J. Biol. Chem. 279:2004;4515-4522
5. Dalton TP, Li Q, Bittel D, Liang L, Andrews GK. Oxidative stress activates metal-responsive transcription factor-1 binding activity. Occupancy in vivo of metal response elements in the metallothionein-I gene promoter. J Biol Chem 1996;271:26233-41.
6. Dineley K.E., Malaiyandi L.M., Reynolds I.J. A reevaluation of neuronal zinc measurements: artifacts associated with high intracellular dye concentration. Mol. Pharmacol. 62:2002;618-627
7. Du S., McLaughlin B., Pal S., Aizenman E. In vitro neurotoxicity of methylisothiazolinone, a commonly used industrial and household biocide, proceeds via a zinc and extracellular signal-regulated kinase mitogen-activated protein kinase-dependent pathway. J. Neurosci. 22:2002;7408-7416
8. Frederickson C. Imaging zinc: old and new tools. Sci. STKE. 182:2003;pe18
9. Giedroc DP, Chen X, Apuy JL. Metal response element (MRE)-binding transcription factor-1 (MTF-1): structure, function, and regulation. Antioxid Redox Signal 2001;3:577-96.
10. Giralt M., Penkowa M., Lago N., Molinero A., Hidalgo J. Metallothionein-1 + 2 protect the CNS after a focal brain injury. Exp. Neurol. 173:2002;114-128
11. 2+-free solutions in rat cortical neurons in vitro. J. Neurochem. 68:1997;1836-1845
12. Heuchel R., Radtke F., Georgiev O., Stark G., Aguet M., Schaffner W. The transcription factor MTF-1 is essential for basal and heavy metal-induced metallothionein gene expression. EMBO J. 13:1994;2870-2875
13. 2+ toxicity and modulation by ZnT-1 in PC12 cells. Brain Res. 886:2000;99-107
14. Koizumi S, Yamada H, Suzuki K, Otsuka F. Zinc-specific activation of a HeLa cell nuclear protein which interacts with a metal responsive element of the human metallothionein-IIA gene. Eur J Biochem 1992;210:555-60.
15. Koizumi S., Suzuki K., Ogra Y., Yamada H., Otsuka F. Transcriptional activity and regulatory protein binding of metal-responsive elements of the human metallothionein-IIA gene. Eur. J. Biochem. 259:1999;635-642
16. Langmade S.J., Ravindra R., Daniels P.J., Andrews G.K. The transcription factor MTF-1 mediates metal regulation of the mouse ZnT1 gene. J. Biol. Chem. 275:2000;34803-34809
17. López E., Figueroa S., Oset-Gasque M.J. González MP Apoptosis and necrosis: two distinct events induced by cadmium in cortical neurons in culture. Br. J. Pharmacol. 138:2003;901-911
18. McLaughlin B., Pal S., Tran M.P., Parsons A.A., Barone F.C., Erhardt J.A., Aizenman E. p38 activation is required upstream of potassium current enhancement and caspase cleavage in thiol oxidant-induced neuronal apoptosis. J. Neurosci. 21:2001;3303-3311
19. Pal S., Hartnett K.A., Nerbonne J.M., Levitan E., Aizenman E. Mediation of neuronal apoptosis by Kv2.1-encoded potassium channels. J. Neurosci. 23:2003;4798-4802
20. Palmiter R.D. Regulation of metallothionein genes by heavy metals appears to be mediated by a zinc-sensitive inhibitor that interacts with a constitutively active transcription factor, MTF-1. Proc. Natl. Acad. Sci. U.S.A. 91:1994;1219-1223
21. Radtke F., Georgiev O., Muller H.P., Brugnera E., Schaffner W. Functional domains of the heavy metal-responsive transcription regulator MTF-1. Nucleic Acids Res. 23:1995;2277-2286
22. Rosenberg P.A., Aizenman E. Hundred-fold increase in neuronal vulnerability to glutamate toxicity in astrocyte-poor cultures of rat cerebral cortex. Neurosci. Lett. 103:1989;162-168
23. Saydam N., Adams T.K., Steiner F., Schaffner W., Freedman J.H. Regulation of metallothionein transcription by the metal-responsive transcription factor MTF-1: identification of signal transduction cascades that control metal-inducible transcription. J. Biol. Chem. 277:2002;20438-20445
24. Smirnova I.V., Bittel D.C., Ravindra R., Jiang H., Andrews G.K. Zinc and cadmium can promote rapid nuclear translocation of metal response element-binding transcription factor-1. J. Biol. Chem. 275:2000;9377-9384
25. Tsuda M., Imaizumi K., Katayama T., Kitagawa K., Wanaka A., Tohyama M., Takagi T. Expression of zinc transporter gene, ZnT-1, is induced after transient forebrain ischemia in the gerbil. J. Neurosci. 17:1997;6678-6684
26. Usai C., Barberis A., Moccagatta L., Marchetti C. Pathways of cadmium influx in mammalian neurons. J. Neurochem. 72:1999;2154-2161
27. van Lookeren Campagne M., Thibodeaux H., van Bruggen N., Cairns B., Gerlai R., Palmer J.T., Williams S.P., Lowe D.G. Evidence for a protective role of metallothionein-1 in focal cerebral ischemia. Proc. Natl. Acad. Sci. U.S.A. 96:1999;12870-12875
28. Westin G., Schaffner W. A zinc-responsive factor interacts with a metal-regulated enhancer element (MRE) of the mouse metallothionein-I gene. EMBO J. 7:1988;3763-3770
29. 2 in vitro is modulated by metallothionein. Mol. Cell. Biol. 23:2003;8471-8485