Distinct roles for de novo versus hydrolytic pathways of sphingolipid biosynthesis in Saccharomyces cerevisiae

Biochemical Journal

Volumn 393, Issue 3, 2006, Pages 733-740

  Cowart, L Ashley ^a   Okamoto, Yasuo ^a,c   Lu, Xinghua ^b   Hannun, Yusuf A ^a  

^a MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

^b MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

^c KAGAWA UNIVERSITY   (Japan)

Author keywords

Ceramide; De novo synthesis; Inositolphosphoceramide phospholipase C (Isc1p); Sphingomyelinase (SMase); Sporulation

Indexed keywords

BIOSYNTHESIS; CARBON; CELLS; HYDROLYSIS; MUTAGENESIS;

CERAMIDE; DE NOVO SYNTHESIS; INOSITOLPHOSPHOCERAMIDE-PHOSPHOLIPASE C (ISC1P); SPHINGOMYELINASE (SMASE); SPORULATION;

YEAST;

CERAMIDE DERIVATIVE; INOSITOLPHOSPHOCERAMIDE PHOSPHOLIPASE C; PHOSPHOLIPASE C; SPHINGOLIPID; SPHINGOLIPID CERAMIDE; SPHINGOMYELIN PHOSPHODIESTERASE; UNCLASSIFIED DRUG;

ARTICLE; CELL CYCLE; CONTROLLED STUDY; ENZYME ACTIVITY; FUNGAL GENE; FUNGAL STRAIN; FUNGUS GROWTH; GENE DELETION; HEATING; HYBRIDIZATION; HYDROLYSIS; ISC1 GENE; LETHALITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; REPRODUCTION; SACCHAROMYCES CEREVISIAE; SPOROGENESIS; STRESS; TEMPERATURE DEPENDENCE; YEAST;

GENE DELETION; GENE EXPRESSION REGULATION, FUNGAL; HEAT; HYDROLYSIS; PHOSPHOLIPASE C; REPRODUCTION; RNA, FUNGAL; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SPHINGOLIPIDS; SPORES, FUNGAL;

FUNGI; MAMMALIA; SACCHAROMYCES CEREVISIAE;

EID: 31544434870     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20050643     Document Type: Article

References (41)

1. Smith, W. L. and Merrill, Jr, A. H. (2002) Sphingolipid metabolism and signaling: minireview series. J. Biol. Chem. 277, 25841-25842
2. Spiegel, S. and Milstien, S. (2003) Sphingosine-1-phosphate: an enigmatic signalling lipid. Nat. Rev. Mol. Cell. Biol. 4, 397-407
3. Reynolds, C. P., Maurer, B. J. and Kolesnick, R. N. (2004) Ceramide synthesis and metabolism as a target for cancer therapy. Cancer Lett. 206, 169-180
4. Sawai, H. and Hannun, Y. A. (1999) Ceramide and sphingomyelinases in the regulation of stress responses. Chem. Phys. Lipids. 102, 141-147
5. Levade, T. and Jaffrezou, J. P. (1999) Signalling sphingomyelinases: which, where, how and why? Biochim. Biophys. Acta. 1438, 1-17
6. Jenkins, G. M., Cowart, L. A., Signorelli, P., Pettus, B. J., Chalfant, C. E. and Hannun, Y. A. (2002) Acute activation of de novo sphingolipid biosynthesis upon heat shock causes an accumulation of ceramide and subsequent dephosphorylation of SR proteins. J. Biol. Chem. 277, 42572-42578
7. Perry, D. K., Carton, J., Shah, A. K., Meredith, F., Uhlinger, D. J. and Hannun, Y. A. (2000) Serine palmitoyltransferase regulates de novo ceramide generation during etoposide-induced apoptosis. J. Biol. Chem. 275, 9078-9084
8. Bose, R., Verheij, M., Haimovitz-Friedman, A., Scotto, K., Fuks, Z. and Kolesnick, R. (1995) Ceramide synthase mediates daunorubicin-induced apoptosis: an alternative mechanism for generating death signals. Cell 82, 405-414
9. Sawai, H., Okamoto, Y., Luberto, C., Mao, C., Bielawska, A., Domae, N. and Hannun, Y. A. (2000) Identification of ISC1 (YER019w) as inositol phosphosphingolipid phospholipase C in Saccharomyces cerevisiae. J. Biol. Chem. 275, 39793-39798
10. Okamoto, Y., Vaena De Avalos, S. and Hannun, Y. A. (2002) Structural requirements for selective binding of ISC1 to anionic phospholipids. J. Biol. Chem. 277, 46470-46477
11. + halotolerance of Saccharomyces cerevisiae. Eur. J. Biochem. 269, 4033-4039
12. Feoktistova, A., Magnelli, P., Abeijon, C., Perez, P., Lester, R. L., Dickson, R. C. and Gould, K. L. (2001) Coordination between fission yeast glucan formation and growth requires a sphingolipase activity. Genetics 158, 1397-1411
13. Vaena de Avalos, S., Su, X., Zhang, M., Okamoto, Y., Dowhan, W. and Hannun, Y. A. (2005) The phosphatidylglycerol/cardiolipin biosynthetic pathway is required for the activation of inositol phosphosphingolipid phospholipase C, Isc1p, during growth of Saccharomyces cerevisiae. J. Biol. Chem. 280, 7170-7177
14. 13a Bligh, E. G. and Dyer, W. J. (1959) A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911-917
15. Preiss, J. E., Loomis, C. R., Bell, R. M. and Niedel, J. E. (1987) Quantitative measurement of sn-1,2-diacylglycerols. Methods Enzymol. 141, 294-300
16. Jenkins, G. M., Richards, A., Wahl, T., Mao, C., Obeid, L. and Hannun, Y. (1997) Involvement of yeast sphingolipids in the heat stress response of Saccharomyces cerevisiae. J. Biol. Chem. 272, 32566-32572
17. Bielawski, J., Hannun, Y. A. and Bielawska, A. (2005) Simultaneous quantitative analysis of bioactive sphingolipids by high performance liquid chromatography-tandem mass spectroscopy. Methods, in the press
18. Jenkins, G. M. and Hannun, Y. A. (2001) Role for de novo sphingoid base biosynthesis in the heat-induced transient cell cycle arrest of Saccharomyces cerevisiae. J. Biol. Chem. 276, 8574-8581
19. Cowart, L. A., Okamoto, Y., Pinto, F. R., Gandy, J. L., Almeida, J. and Hannun, Y. A. (2003) Roles for sphingolipid biosynthesis in mediation of specific programs of the heat stress response determined through gene expression profiling. J. Biol. Chem. 278, 30328-30338
20. Burchett, S. A., Flanary, P., Aston, C., Jiang, L., Young, K. H., Uetz, P., Fields, S. and Dohlman, H. G. (2002) Regulation of stress response signaling by the N-terminal dishevelled/EGL-10/pleckstrin domain of Sst2, a regulator of G protein signaling in Saccharomyces cerevisiae. J. Biol. Chem. 277, 22156-22167
21. Skrzypek, M. S., Nagiec, M. M., Lester, R. L. and Dickson, R. C. (1999) Analysis of phosphorylated sphingolipid long-chain bases reveals potential roles in heat stress and growth control in Saccharomyces. J. Bacteriol. 181, 1134-1140
22. Ferguson-Yankey, S. R., Skrzypek, M. S., Lester, R. L. and Dickson, R. C. (2002) Mutant analysis reveals complex regulation of sphingolipid long chain base phosphates and long chain bases during heat stress in yeast. Yeast 19, 573-586
23. Dickson, R. C., Nagiec, E. E., Skrzypek, M., Tillman, P., Wells, G. B. and Lester, R. L. (1997) Sphingolipids are potential heat stress signals in Saccharomyces. J. Biol. Chem. 272, 30196-30200
24. Wells, G. B., Dickson, R. C. and Lester, R. L. (1998) Heat-induced elevation of ceramide in Saccharomyces cerevisiae via de novo synthesis. J. Biol. Chem. 273, 7235-7243
25. Hearn, J. D., Lester, R. L. and Dickson, R. C. (2003) The uracil transporter Fur4p associates with lipid rafts. J. Biol. Chem. 278, 3679-3686
26. Jenkins, G. M. (2003) The emerging role for sphingolipids in the eukaryotic heat shock response. Cell Mol. Life. Sci. 60, 701-710
27. MacIver, F. H., Dawes, I. W. and Grant, C. M. (1997) Identification of a Saccharomyces cerevisiae mitochondrial-DNA which can act as a promoter tightly regulated by carbon source when placed in the nucleus. Curr. Genet. 31, 119-121
28. Dickson, R. C. and Lester, R. L. (2002) Sphingolipid functions in Saccharomyces cerevisiae. Biochim. Biophys. Acta 1583, 13-25
29. Marchesini, N., Osta, W., Bielawski, J., Luberto, C., Obeid, L. M. and Hannun, Y. A. (2004) Role for mammalian neutral sphingomyelinase 2 in confluence-induced growth arrest of MCF7 cells. J. Biol. Chem. 279, 25101-25111
30. Choi, J. Y. and Martin, C. E. (1999) The Saccharomyces cerevisiae FAT1 gene encodes an acyl-CoA synthetase that is required for maintenance of very long chain fatty acid levels. J. Biol. Chem. 274, 4671-4683
31. Ding, Y. and Wilkins, D. (2004) The effect of normalization on microarray data analysis. DNA Cell Biol. 23, 635-642
32. Xu, L., Maresh, G. A., Giardina, J. and Pincus, S. H. (2004) Comparison of different microarray data analysis programs and description of a database for microarray data management. DNA Cell Biol. 23, 643-651
33. Ren, B., Robert, F., Wyrick, J. J., Aparicio, O., Jennings, E. G., Simon, I., Zeitlinger, J., Schreiber, J., Hannett, N., Kanin, E. et al. (2000) Genome-wide location and function of DNA binding proteins. Science 290, 2306-2309
34. Bagnat, M. and Simons, K. (2002) Cell surface polarization during yeast mating. Proc. Natl. Acad. Sci. U.S.A. 99, 14183-14188
35. 2+ concentrations at 37°C, is required for mannosylation of inositolphosphorylceramide. Mol. Gen. Genet. 255, 570-579
36. Cliften, P., Wang, Y., Mochizuki, D., Miyakawa, T., Wangspa, R., Hughes, J. and Takemoto, J. Y. (1996) SYR2, a gene necessary for syringomycin growth inhibition of Saccharomyces cerevisiae. Microbiology 142, 477-484
37. Gustin, M. C., Albertyn, J., Alexander, M. and Davenport, K. (1998) MAP kinase pathways in the yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 62, 1264-1300
38. Pillai, B., Verma, J., Abraham, A., Francis, P., Kumar, Y., Tatu, U., Brahmachari, S. K. and Sadhale, P. P. (2003) Whole genome expression profiles of yeast RNA polymerase II core subunit, Rpb4, in stress and nonstress conditions. J. Biol. Chem. 278, 3339-3346
39. Cole, G. M. and Reed, S. I. (1991) Pheromone-induced phosphorylation of a G protein beta subunit in S. cerevisiae is associated with an adaptive response to mating pheromone. Cell. 64, 703-716
40. Chang, Y., Abe, A. and Shayman, J. A. (1995) Ceramide formation during heat shock: a potential mediator of αB-crystallin transcription. Proc. Natl. Acad. Sci. U.S.A. 92, 12275-12279
41. Kondo, T., Matsuda, T., Tashima, M., Umehara, H., Domae, N., Yokoyama, K., Uchiyama, T. and Okazaki, T. (2000) Suppression of heat shock protein-70 by ceramide in heat shock-induced HL-60 cell apoptosis. J. Biol. Chem. 275, 8872-8879