HTS Compatible Assay for Antioxidative Agents Using Primary Cultured Hepatocytes

Assay and Drug Development Technologies

Volumn 1, Issue 3, 2003, Pages 469-477

  Gaunitz, Frank ^a   Heise, Kerstin ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIOXIDANT; CASP3 PROTEIN, RAT; CASPASE; CASPASE 3; CASPASE 7; FISETIN; FLAVONOID;

ANIMAL; ARTICLE; BIOASSAY; CELL CULTURE; CELL DIVISION; CELL SURVIVAL; COMPARATIVE STUDY; DRUG EFFECT; LIVER CELL; MALE; METABOLISM; METHODOLOGY; OXIDATIVE STRESS; RAT; SPRAGUE DAWLEY RAT;

ANIMALS; ANTIOXIDANTS; BIOLOGICAL ASSAY; CASPASE 3; CASPASE 7; CASPASES; CELL DIVISION; CELL SURVIVAL; CELLS, CULTURED; FLAVONOIDS; HEPATOCYTES; MALE; OXIDATIVE STRESS; RATS; RATS, SPRAGUE-DAWLEY;

EID: 3142761468     PISSN: 1540658X     EISSN: 15578127     Source Type: Journal    
DOI: 10.1089/154065803322163786     Document Type: Article

References (22)

1. Kohen R, Nyska A: Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicol Pathol 2002;30:620-650.
2. Esposito E, Rotilio D, Di Matteo V, Di Giulio C, Cacchio M, Algeri S: A review of specific dietary antioxidants and the effects on biochemical mechanisms related to neurodegenerative processes. Neurobiol Aging 2002;23:719-735.
3. Mattson MP, Liu D: Energetics and oxidative stress in synaptic plasticity and neurodegenerative disorders. Neuromol Med 2002;2:215-231.
4. Sorescu D, Griendling KK: Reactive oxygen species, mitochondria, and NAD(P)H oxidases in the development and progression of heart failure. Congest Heart Fail 2002;8:132-140.
5. Epe B: Role of endogenous oxidative DNA damage in carcinogenesis: what can we learn from repair-deficient mice? Biol Chem 2002;383:467-475.
6. Kang DH: Oxidative stress, DNA damage, and breast cancer. AACN Clin Issues 2002;13:540-549.
7. Benhar M, Engelberg D, Levitzki A: ROS, stress-activated kinases and stress signaling in cancer. EMBO Rep 2002;3:420-425.
8. Parola M, Robino G: Oxidative stress-related molecules and liver fibrosis. J Hepatol 2001;35:297-306.
9. Gebhardt R: Oxidative stress, plant-derived antioxidants and liver fibrosis. Planta Med 2002;68:289-296.
10. Feng Q, Kumagai T, Torii Y, Nakamura Y, Osawa T, Uchida K: Anticarcinogenic antioxidants as inhibitors against intracellular oxidative stress. Free Radic Res 2001;35:779-788.
11. Prasad KN, Cole WC, Hovland AR, Prasad KC, Nahreini P, Kumar B, Edwards-Prasad J, Andreatta CP: Multiple antioxidants in the prevention and treatment of neurodegenerative disease: analysis of biologic rationale. Curr Opin Neurol 1999;12:761-770.
12. Jaeschke H, Gores GJ, Cederbaum AI, Hinson JA, Pessayre D, Lemasters JJ: Mechanisms of hepatotoxicity. Toxicol Sci 2002;65:166-176.
13. Burger HJ, Gebhardt R, Mayer C, Mecke D: Different capacities for amino acid transport in periportal and perivenous hepatocytes isolated by digitonin/collagenase perfusion. Hepatology 1989;9:22-28.
14. Zhang JH, Chung TD, Oldenburg KR: A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 1999;4:67-73.
15. Gebhardt R, Jung W: Biliary secretion of sodium fluorescein in primary monolayer cultures of adult rat hepatocytes and its stimulation by nicotinamide. J Cell Sci 1982;56:233-244.
16. Cougnon M, Benammou S, Brouillard F, Hulin P, Planelles G: Effect of reactive oxygen species on NH41 permeation in Xenopus laevis oocytes. Am J Physiol Cell Physiol 2002;282:C1445-C1453.
17. Martin C, Martinez R, Navarro R, Ruiz-Sanz JI, Lacort M, Ruiz-Larrea MB: tert-Butyl hydroperoxide-induced lipid signaling in hepatocytes: involvement of glutathione and free radicals. Biochem Pharmacol 2001;62:705-712.
18. Dubuisson ML, De Wergifosse B, Kremers P, Marchand-Brynaert J, Trouet A, Rees JF: Protection against nitrofurantoin-induced oxidative stress by coelenterazine analogues and their oxidation products in rat hepatocytes. Free Radic Res 2001;34:285-296.
19. Liu CL, Wang JM, Chu CY, Cheng MT, Tseng TH: In vivo protective effect of protocatechuic acid on tert-butyl hydroperoxide-induced rat hepatotoxicity. Food Chem Toxicol 2002;40:635-631.
20. Jimenez M, Escribano-Cebrian J, Garcia-Carmona F: Oxidation of the flavonol fisetin by polyphenol oxidase. Biochim Biophys Acta 1998;1425:534-542.
21. Sugihara N, Arakawa T, Ohnishi M, Furuno K: Anti- and pro-oxidative effects of flavonoids on metal-induced lipid hydroperoxide-dependent lipid peroxidation in cultured hepatocytes loaded with alpha-linolenic acid. Free Radic Biol Med 1999;27:1313-1323.
22. Lee WR, Shen SC, Lin HY, Hou WC, Yang LL, Chen YC: Wogonin and fisetin induce apoptosis in human promyeloleukemic cells, accompanied by a decrease of reactive oxygen species, and activation of caspase 3 and Ca(2+)-dependent endonuclease. Biochem Pharmacol 2002;63:225-236.