Purification and characterization of three immunodominant proteins (38, 30, and 16 kDa) of Mycobacterium tuberculosis

Protein Expression and Purification

Volumn 24, Issue 2, 2002, Pages 188-195

  Uma Devi, K R ^a   Senthil Kumar, K S ^a   Ramalingam, B ^a   Alamelu, Raja ^a  

^a NATIONAL INSTITUTE FOR RESEARCH IN TUBERCULOSIS   (India)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; EPITOPE;

ARTICLE; CHEMISTRY; CIRCULAR DICHROISM; ENZYME LINKED IMMUNOSORBENT ASSAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IMMUNOLOGY; ISOELECTRIC POINT; ISOLATION AND PURIFICATION; MYCOBACTERIUM TUBERCULOSIS; TWO DIMENSIONAL GEL ELECTROPHORESIS;

BACTERIAL PROTEINS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; IMMUNODOMINANT EPITOPES; ISOELECTRIC POINT; MYCOBACTERIUM TUBERCULOSIS;

MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS;

EID: 0036515892     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1569     Document Type: Article

References (29)

1. Enarson, D., and Murray, J. (1996) Global epidemiology of tuberculosis, in "Tuberculosis" (Rom, W. N., and Garay, S., Eds.), pp. 57-75, Little, Brown, and Co.
2. Raviglione, M. C., Snider, D. J., and Kochi, A. (1995) Global epidemiology of tuberculosis. Morbidity and mortality of a worldwide epidemic. JAMA 273, 220-226.
3. World Health Organization (1998) Antituberculosis drug resistance worldwide. 73, 249-256.
4. Chan, E. D., Heifets, L., and Iseman, M. D. (2000) Immunologic diagnosis of tuberculosis: A review. Tuberc. Lung Dis. 80, 131-140.
5. Daniel, T., and Debanne, S. (1987) The serodiagnosis of tuberculosis and other mycobacterial diseases by enzyme-linked immunosorbent assay. Am. Rev. Respir. Dis. 135, 1137-1151.
6. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
7. Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354.
8. O'Farrell, P. H. (1975) High-resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250, 4007-4021.
9. Daniel, T. M., and Anderson, P. A. (1978) The isolation by immunosorbent affinity chromatography and physicochemical characterization of Mycobacterium tuberculosis antigen 5. Am. Rev. Respir. Dis. 117, 533-539.
10. Young, D., Kent, L., Rees, A., Lamb, J., and Ivanyi, J. (1986) Immunological activity of a 38-kilodalton protein purified from Mycobacterium tuberculosis. Infect. Immun. 54, 177-183.
11. Kadival, G. V., and Chaparas, D. (1987) Production, characterization and species specificity of five monoclonal antibodies to Mycobacterium tuberculosis. J. Clin. Microbiol. 25, 76-80.
12. Espitia, C., Cervera, I., Gonzalez, R., and Mantilla, R. (1989) A 38-kD Mycobacterium tuberculosis antigen associated with infection. Its isolation and serologic evaluation. Clin. Exp. Immunol. 77, 373-377.
13. Singh, M., Andersen, A. B., McCarthy, J. E. G., Rohde, M., Schutte, H., Sanders, E., and Timmis, K. N. (1992) The Mycobacterium tuberculosis 38kDa antigen: Over expression of the gene in Escherichia coli and purification and characterisation of the recombinant product. Gene 117, 53-60.
14. Bassey, E. O. E., Catty, D., Kumararatne, D. S., and Raykundalia, C. (1996) Candidate antigens for improved serodiagnosis of tuberculosis. Tuberc. Lung Dis. 77, 136-145.
15. Jackett, P. S., Graham, H. P., Harsh, V. B., Mistry, A., Young, B. D., and Ivanyi, J. (1988) Specificity of antibodies to immunodominant mycobacterial antigens in pulmonary tuberculosis. J. Clin. Microbiol. 26, 2313-2318.
16. Bothamley, G. H., Rudd, R., Festenstein, F., and Ivanyi, J. (1992) Clinical value of the measurement of Mycobacterium tuberculosis specific antibody in pulmonary tuberculosis. Thorax 47, 270-275.
17. Nagai, S., Wiker, H. G., Harboe, M., and Kinomoto, M. (1991) Isolation and partial characterization of major protein antigens in the culture fluid of Mycobacterium tuberculosis. Infect. Immun. 59, 372-382.
18. Thole, J. E. R., Schoningh, R., Janson, A. A. M., Garbe, T., Cornelisse, Y. E., Clark-Curtiss, J. E., Kolk, A. H. J., Ottenhoff, T. H. M., De Vries, R. R. P., and Abou-Zeid, C. (1992) Molecular and immunological analysis of a fibronectin-binding protein antigen secreted by Mycobacterium leprae. Mol. Microbiol. 6, 153-163.
19. Wiker, H. G., and Harboe, M. (1992) The antigen 85 complex: A major secretion product of Mycobacterium tuberculosis. Microbiol. Rev. 56, 648-661.
20. Daniel, T. M., and Ferguson, L. E. (1970) Purification and characterization of two proteins from culture nitrates of Mycobacterium tuberculosis H37Ra strain. Infect. Immun. 1, 164-168.
21. Lim, J. H., Park, J. K., Jo, E. K., Song, C. H., Dullei, M., Song, Y. J., and Kim, H. J. (1999) Purification and immunoreactivity of three components from the 30/32-kilodalton antigen 85 complex in Mycobacterium tuberculosis. Infect. Immun. 67, 6187-6190.
22. Van Vooren, J. P., Drowart, A., de Cock, M., van Onckelen, M. H., D'Hoop, M. H., Yernault, J. C., Valcke, C., and Huygen, K. (1991) Humoral immune response of tuberculous patients against the three components of the Mycobacterium bovis BCG 85 complex separated by isoelectric focusing. J. Clin. Microbiol. 29, 2348-2350.
23. Drowart, A., Launois, P., De Cock, J., Yernault, J., and Van Vooren, J. P. (1991) An isoelectric focusing method for the study of the humoral response against the antigen 85 complex of Mycobacterium bovis BCG in the different forms of leprosy. J. Immunol. Methods 145, 223-228.
24. Lee, B. Y., Hefta, S. A., and Brennan, P. J. (1992) Characterization of the major membrane protein of virulent Mycobacterium tuberculosis. Infect. Immun. 60, 2066-2074.
25. Garbe, T. R., Hibler, N. S., and Deretic, V. (1999) Response to reactive nitrogen intermediates in Mycobacterium tuberculosis: Induction of the 16-kilodalton α-crystallin homolog by exposure to nitric oxide donors. Infect. Immun. 67, 460-465.
26. Verbon, A., Hartskeerl, R. A., Schuitema, A., Kolk, A. H. J., Young, D. B., and Lathigra, R. (1992) The 14,000 molecular weight antigen of Mycobacterium tuberculosis is related to the α-crystallin family of low-molecular weight heat shock proteins. J. Bacteriol. 174, 1352-1359.
27. Berengian, A. R., Parfenova, M., and Mchaourab, H. S. (1999) Site-directed spin labeling study of subunit interactions in the α-crystallin domain of small heat-shock proteins - Comparison of the oligomer symmetry in α-crystallin, HSP 27 and HSP 16.3. J. Biol. Chem. 274, 6305-6314.
28. Yuan, Y., Crane, D. D., Simpson, R. M., Zhu, Y., Hickey, M. J., Sherman, D. R., and Barry, C. E. (1998) The 16 kDa α-crystallin (Acr) protein of Mycobacterium tuberculosis is required for growth in macrophages. Proc. Natl. Acad. Sci. USA 95, 9578-9583.
29. Chang, Z., Primm, T. P., Jakana, J., Lee, I. H., Serysheval, I., Chiu, W., Gilbert, H. F., and Quiocho, F. A. (1996) Mycobacterium tuberculosis 16 kDa antigen (Hspl6.3) functions as an oligomeric structure in vitro to suppress thermal aggregation. J. Biol. Chem. 271, 7218-7223.