Crystal structure of a major secreted protein of Mycobacterium tuberculosis - MPT63 at 1.5-Å resolution

Protein Science

Volumn 11, Issue 12, 2002, Pages 2887-2893

  Goulding, Celia W ^a   Parseghian, Angineh ^a   Sawaya, Michael R ^a   Cascio, Duilio ^a   Apostol, Marcin I ^a   Gennaro, Maria Laura ^b   Eisenberg, David ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b PUBLIC HEALTH RESEARCH INSTITUTE   (United States)

Author keywords

Cell host interactions; Mycobacterium tuberculosis; Secreted protein MPT63; TB drug target; X ray crystal structure

Indexed keywords

BACTERIAL PROTEIN; INVASIN; RETINA S ANTIGEN; MPT63 ANTIGEN, MYCOBACTERIUM TUBERCULOSIS;

ARTICLE; BACTERIAL GENOME; BACTERIAL VIRULENCE; BETA SHEET; CELL INTERACTION; CRYSTAL STRUCTURE; DRUG TARGETING; ENDOCYTOSIS; HOST CELL; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PHAGOCYTOSIS; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN STRUCTURE; SEQUENCE HOMOLOGY; X RAY CRYSTALLOGRAPHY; AMINO ACID SEQUENCE; CHEMICAL STRUCTURE; CHEMISTRY; ELECTRICITY; METABOLISM; MOLECULAR GENETICS; PROTEIN SECONDARY STRUCTURE;

BACTERIA (MICROORGANISMS); BOVINAE; CORYNEBACTERINEAE; HOMO; HOMO SAPIENS; MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS; YERSINIA; YERSINIA PSEUDOTUBERCULOSIS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CRYSTALLOGRAPHY, X-RAY; ELECTROSTATICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MYCOBACTERIUM TUBERCULOSIS; PROTEIN STRUCTURE, SECONDARY; SEQUENCE HOMOLOGY;

EID: 0036891686     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.0219002     Document Type: Article

References (28)

1. Anderson, D.H., Harth, G., Horwitz, M.A., and Eisenberg, D. 2001. An interfacial mechanism and a class of inhibitors inferred from two crystal structures of the Mycobacterium tuberculosis 30 kDa major secretory protein (Antigen 85B), a mycolyl transferase. J. Mol. Biol. 307: 671-681.
2. Braunstein, M., Griffin, T.I., Kriakov, J.I., Friedman, S.T., Grindley, N.D., and Jacobs, Jr., W.R., 2000. Identification of genes encoding exported Mycobacterium tuberculosis proteins using a Tn552′phoA in vitro transposition system. J. Bacteriol. 182: 2732-2740.
3. Cole, S.T., Eiglmeier, K., Parkhill, J., James, K.D., Thomson, N.R., Wheeler, P.R., Honore, N., Gamier, T., Churcher, C., Harris, D., et al. 2001. Massive gene decay in the leprosy bacillus. Nature 409: 1007-1011.
4. Collaborative Computational Project. 1994. The CCP4 Suite: Programs for protein crystallography. Acta Crystallogr. D. Biol. Crystallogr. 50: 760-763.
5. Cowtan, K. and Main, P. 1998. Miscellaneous algorithms for density modification. Acta Crystallogr. D. Biol. Crystallogr. 54: 487-493.
6. Gomez, M., Johnson, S., and Gennaro, M.L. 2000. Identification of secreted proteins of Mycobacterium tuberculosis by a bioinformatic approach. Infect. Immun. 68: 2323-2327.
7. Halaby, D.M. and Momon, J.P. 1998. The immunoglobulin superfamily: An insight on its tissular, species, and functional diversity. J. Mol. Evol. 46: 389-400.
8. Hamburger, Z.A., Brown, M.S., Isberg, R.R., and Bjorkman, P.J. 1999. Crystal structure of invasin: A bacterial integrin-binding protein. Science 286: 291-295.
9. Hirsch, J.A., Schubert, C., Gurevich, V.V., and Sigler, P.B. 1999. The 2.8 Å crystal structure of visual arrestin: A model for arrestin's regulation. Cell 97: 257-269.
10. Holm, L. and Sander, C. 1993. Protein structure comparison by alignment of distance matrices. J. Mol. Biol. 233: 123-138.
11. Jones, T.A., Zou, J.Y., Cowan, S.W., and Kjeldgaard, M. 1991. Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallogr. A 47: 110-119.
12. Lee, B.Y. and Horwitz, M.A. 1999. T-cell epitope mapping of the three most abundant extracellular proteins of Mycobacterium tuberculosis in outbred guinea pigs. Infect. Immun. 67: 2665-2670.
13. Mallick, P., Goodwill, K.E., Fitz-Gibbon, S., Miller, J.H., and Eisenberg, D. 2000. Selecting protein targets for structural genomics of Pyrobaculum aerophilum: Validating automated fold assignment methods by using binary hypothesis testing. Proc. Natl. Acad. Sci. 97: 2450-2455.
14. Manca, C., Lyashchenko, K., Wiker, H.G., Usai, D., Colangeli, R., and Gennaro, M.L. 1997. Molecular cloning, purification, and serological characterization of MPT63, a novel antigen secreted by Mycobacterium tuberculosis. Infect. Immun. 65: 16-23.
15. Nagai, S., Wiker, H.G., Harboe, M., and Kinomoto, M. 1991. Isolation and partial characterization of major protein antigens in the culture fluid of Mycobacterium tuberculosis. Infect. Immun. 59: 372-382.
16. Otwinowski, Z. and Minor, W. 1996. Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol., 276A: 307-326.
17. Owen, D.J. and Luzio, J.P. 2000. Structural insights into clathrin-mediated endocytosis. Curr. Opin. Cell. Biol. 12: 467-474.
18. Owen, D.J., Vallis, Y., Pearse, B.M., McMahon, H.T., and Evans, P.R. 2000. The structure and function of the β 2-adaptin appendage domain. EMBO J. 19: 4216-4227.
19. Perrakis, A., Morris, R., and Lamzin, V.S. 1999. Automated protein model building combined with iterative structure refinement. Nat. Struct. Biol. 6: 458-463.
20. Ronning, D.R., Klabunde, T., Besra, G.S., Vissa, V.D., Belisle, J.T., and Sacchettini, J.C. 2000. Crystal structure of the secreted form of antigen 85C reveals potential targets for mycobacterial drugs and vaccines. Nat. Struct. Biol. 7: 141-146.
21. Shapiro, L., Kwong, P.D., Fannon, A.M., Colman, D.R., and Hendrickson, W.A. 1995. Considerations on the folding topology and evolutionary origin of cadherin domains. Proc. Natl. Acad. Sci. 92: 6793-6797.
22. Sharp, K.A., Nicholls, A., Friedman, R., and Honig, B. 1991. Extracting hydrophobic free energies from experimental data: Relationship to protein folding and theoretical models. Biochemistry 30: 9686-9697.
23. Shindyalov, I.N. and Bourne, P.E. 1998. Protein structure alignment by incremental combinatorial extension (CE) of the optimal path. Protein Eng. 11: 739-747.
24. Van Duyne, G.D., Standaert, R.F., Karplus, P.A., Schreiber, S.L., and Clardy, J. 1993. Atomic structures of the human immunophilin FKBP-12 complexes with FK506 and rapamycin. J. Mol. Biol. 229: 105-124.
25. Wiker, H.G., Harboe, M., and Nagai, S. 1991. A localization index for distinction between extracellular and intracellular antigens of Mycobacterium tuberculosis. J. Gen. Microbiol. 137: 875-884.
26. Wiker, H.G., Wilson, M.A., and Schoolnik, G.K. 2000. Extracytoplasmic proteins of Mycobacterium tuberculosis - Mature secreted proteins often start with aspartic acid and proline. Microbiology 146: 1525-1533.
27. The World Health Organization. 1998. The World Health Report 1998: Life in the 21st Century - A Vision for All. Geneva, Switzerland.
28. Young, D., Garbe, T., Lathigra, R., Abou-Zeid, C., and Zhang, Y. 1991. Characterization of prominent protein antigens from mycobacteria. Bull. Int. Union Tuberc. Lung Dis. 66: 47-51.