A novel flow cytometric assay focusing on perforin release mechanisms of cytotoxic T lymphocytes

Journal of Immunological Methods

Volumn 289, Issue 1-2, 2004, Pages 17-26

  Weren, Andrea ^a   Bonnekoh, Bernd ^a   Schraven, Burkhart ^a   Gollnick, Harald ^a   Ambach, Andreas ^a  

^a OTTO VON GUERICKE UNIVERSITY   (Germany)

Author keywords

Atopic; BLT; CD3 crosslinking; CTL; cytotoxic T lymphocytes; Cytotoxicity; FCS; fetal calf serum; Flow cytometry; Lytic granules; mean fluorescence intensity; MFI; NA CBZ L lysine thiobenzyl ester hydrochloride; phorbol 12 myristate 13 acetate; PMA

Indexed keywords

CD3 ANTIGEN; CD63 ANTIGEN; CD8 ANTIGEN; ESTERASE; FICOLL; IONOMYCIN; PERFORIN; PHORBOL 13 ACETATE 12 MYRISTATE;

ADULT; ARTICLE; CELL SURFACE; CONTROLLED STUDY; CORRELATION ANALYSIS; CROSS LINKING; CYTOTOXIC T LYMPHOCYTE; DEGRANULATION; FEMALE; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; HOMEOSTASIS; HUMAN; HUMAN EXPERIMENT; IMMUNITY; MALE; NORMAL HUMAN; PERIPHERAL LYMPHOCYTE; PRIORITY JOURNAL; REDUCTION; UPREGULATION;

ANTIGENS, CD3; BIOLOGICAL ASSAY; CELL DEGRANULATION; CYTOPLASMIC GRANULES; FLOW CYTOMETRY; HUMANS; IONOMYCIN; LYMPHOCYTE ACTIVATION; MEMBRANE GLYCOPROTEINS; PORE FORMING CYTOTOXIC PROTEINS; PROTEIN TRANSPORT; T-LYMPHOCYTES, CYTOTOXIC; TETRADECANOYLPHORBOL ACETATE;

EID: 3142532436     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2004.01.028     Document Type: Article

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