Granzyme B ELISPOT assay for ex vivo measurements of T cell immunity

Journal of Immunological Methods

Volumn 240, Issue 1-2, 2000, Pages 143-155

  Rininsland, Frauke H ^a   Helms, Thomas ^a,c   Asaad, Robert J ^b   Boehm, Bernhard O ^c   Tary Lehmann, Magdalena ^a  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b CASE WESTERN RESERVE UNIVERSITY   (United States)

^c UNIVERSITY HOSPITAL ULM   (Germany)

Author keywords

Cytotoxic T cells; ELISPOT; Granzyme B; Memory; Peptide specificity

Indexed keywords

GRANZYME B;

ANIMAL CELL; ARTICLE; CELLULAR IMMUNITY; CHO CELL; DIAGNOSTIC VALUE; ENZYME LINKED IMMUNOSORBENT ASSAY; IMAGE ANALYSIS; NONHUMAN; PRIORITY JOURNAL; T LYMPHOCYTE;

ANIMALS; BASE SEQUENCE; CELL LINE; CHO CELLS; CHROMIUM; CLONING, MOLECULAR; CRICETINAE; CYTOTOXICITY TESTS, IMMUNOLOGIC; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; GRANZYMES; HIV ANTIGENS; HUMANS; IMMUNOLOGIC MEMORY; LYMPHOCYTE ACTIVATION; MOLECULAR SEQUENCE DATA; SERINE ENDOPEPTIDASES; T-LYMPHOCYTES, CYTOTOXIC;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS;

EID: 0034705645     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(00)00191-5     Document Type: Article

References (24)

1. Altman J.D., Moss P.A.H., Guolder P.J.R., Barouch D.H., McHeyzer-Williams M.G., Bell J.I., McMichael A.J., Davis M.M. Phenotypic analysis of antigen-specific T lymphocytes. Science. 274:1996;94-96.
2. Bjorkman P.J., Parham P. Structure, function and diversity of class I major histocompatibility complex molecules. Annu. Rev. Biochem. 59:1990;253.
3. Carter L.L., Swain S.L. Single cell analyses of cytokine production. Curr. Opin. Immunol. 9:1997;177.
4. Chen W., Anton L.W., Bennink J.R., Yewdell J.W. Dissecting the multifactorial causes of immunodominance in class I restricted T cell responses to viruses. Immunity. 12:2000;83.
5. Engelhard V.H. Structure of peptides associated with class I and class II MHC molecules. Annu. Rev. Immunol. 12:1994;181.
6. Falk K., Rotzschke O., Stevanovic S., Jung G., Rammensee H.G. Allele-specific motivs revealed by sequencing of self-peptides eluted from MHC molecules. Nature. 351:1991;290.
7. Fujihashi K., McGhee J.R., Beagley K.W., McPherson D.T., McPherson S.A., Huang C.-H., Kiyono H. Cytokine-specific ELISPOT assay. Single cell analysis of IL-2, IL-4 and IL-6 producing cells. J. Immunol. Methods. 160:1993;181.
8. Grabstein K., Chen Y.U. Mishell B.B., Shiigi S.M., Henry C., Mishell R.I. Selected Methods in Cellular Immunology. 1980;124 Freeman, New York.
9. Griffiths G.M. The cell biology of CTL killing. Curr. Opin. Immunol. 7:1995;343.
10. Gross D.M., Forsthuber T., Tary-Lehmann M., Etling C., Ito K., Nagy Z.A., Field J.A., Steere A.C., Hubert B.T. Identification of LFA-1 as a candidate autoantigen in treatment-resistant Lyme arthritis. Science. 281:1998;703.
11. Heeger P.S., Greenspan N.S., Kuhlenschmidt S., Dejelo C., Hricik D.E., Shulak J.A., Tary-Lehmann M. Pretransplant frequency of donor-specific, IFN-γ-producing lymphocytes is a manifestation of immunologic memory and correlates with the risk of posttransplant rejection. J. Immunol. 163:1999;2267.
12. Heeger, P.S., Valujskikh, A., Lehmann, P., 2000. Comprehensive assessment of determinant specificity, frequency, and cytokine signature of the primer CD8+ repertoire induced by a minor transplantation antigen. J. Immunol. (in press).
13. Henkart P.A. Lymphocyte mediated cytotoxicity:Two pathways and multiple effector molecules. Immunity. 1:1994;343.
14. Johnson T.R., Trojan J., Rudin S.D., Ilan J., Tykocinski M.L., Ilan J. Evoking an immune response to glioblastoma cells transfected with episome based plasmid expressing transcripts to insulin-like growth factor I. Levine A.J., Schmidek H.H. Molecular Genetics of Nervous System Tumors. Vol. 35:1993;387 Wiley-Liss, New York.
15. Karulin A.Y., Hesse M.D., Tary-Lehmann M., Lehmann P.V. Single cytokine-producing memory cells predominate in type 1 and type 2 immunity. J. Immunol. 164:2000;1862.
16. Murali-Krishna K., Altman J.D., Suresh M., Sourdive D.J.D., Zajac A.J., Miller J.D., Slansky J., Ahmed R. Counting antigen-specific CD8 T cells: A reevaluation of bystander activation during viral infection. Immunity. 8:1998;177.
17. Openshaw P., Murphy E., Hosken N., Maino V., Davis K., Murphy K., O'Garra A. Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper I and T helper 2 populations. J. Exp. Med. 182:1995;1357.
18. Panina-Bordignon P., Lang R., van Endert P.M., Benazzi E., Felix A.M., Pastore R.M., Spinas G.A., Sinigaglia F. Cytotoxic T cells specific for glutamic acid decarboxylase in autoimmune diabetes. J. Exp. Med. 181:1995;1923.
19. Sette A., Sidney J. HLA supertypes and supermotifs: a functional perspective on HLA polymorphism. Curr. Opin. Immunol. 10:1998;478.
20. Shresta S., Pham C.T.M., Thomas D.A., Graubert T.A., Ley T. How do cytotoxic lymphocytes kill their targets? Curr. Opin. Immunol. 10:1998;581.
21. Tanguay S., Killion J. Direct comparison of ELISPOT and ELISA-based assays for detection of individual cytokine-secreting cells. Lymphokine Cytokine Res. 13:1994;259.
22. Targoni O., Lehmann P.V. Endogenous myelin basic protein inactivates the high avidity T cell repertoire. J. Exp. Med. 187:1998;2055.
23. + T cell clones. J. Immunol. 145:1990;2092.
24. Trapani J.A., Klein J.L., White P.C., DuPont B. Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes. Proc. Natl. Acad. Sci. USA. 85:1988;6924.