Spectral identification of specific photophysics of Cy5 by means of ensemble and single molecule measurements

Journal of Physical Chemistry A

Volumn 110, Issue 1, 2006, Pages 45-50

  Huang, Zhengxi ^a   Ji, Dongmei ^a   Wang, Sufan ^a   Xia, Andong ^a   Koberling, Felix ^b   Patting, Matthias ^b   Erdmann, Rainer ^b  

^a INSTITUTE OF CHEMISTRY   (China)

^b PICOQUANT GMBH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ELECTRIC EXCITATION; ELECTRON RESONANCE; FLUORESCENCE; ISOMERIZATION; SPECTROSCOPIC ANALYSIS; SPECTRUM ANALYSIS;

MOLECULE SPECTROSCOPIC APPLICATIONS; SINGLE MOLECULE MEASUREMENTS; SPECIFIC PHOTOPHYSICS; TRANS-CIS ISOMERIZATION;

MOLECULAR DYNAMICS;

CARBOCYANINE; CYANINE DYE 5;

ARTICLE; CHEMISTRY; FLUORESCENCE RESONANCE ENERGY TRANSFER; METHODOLOGY; PHOTOCHEMISTRY; SENSITIVITY AND SPECIFICITY; STEREOISOMERISM; TIME;

CARBOCYANINES; FLUORESCENCE RESONANCE ENERGY TRANSFER; PHOTOCHEMISTRY; SENSITIVITY AND SPECIFICITY; STEREOISOMERISM; TIME FACTORS;

EID: 31144474579     PISSN: 10895639     EISSN: None     Source Type: Journal    
DOI: 10.1021/jp0562936     Document Type: Article

References (57)

1. Alexander, K. C.; Semen, V. S.; Helmut, G. J. Photochem. Photobiol., A 2001, 141, 39-45.
2. Armitage, B.; O'Brien, D. F. J. Am. Chem. Soc. 1992, 114, 7396-7403.
3. DiPaolo, R. E.; Scaffardi, L. B.; Duchowicz, R.; Bilmes, G. M. J. Phys. Chem. 1995, 99, 13796-13799.
4. Ghelli, S.; Ponterini, G. J. Mol. Struct. 1995, 355, 193-200.
5. Heilemann, M.; Margeat, E.; Kasper, R.; Sauer, M.; Tinnefeid, P. J. Am. Chem. Soc. 2005, 127, 3801-3806.
6. Kanony, C.; Akerman, B.; Tuite, E. J. Am. Chem. Soc. 2001, 123, 7985-7995.
7. Khimenko, V.; Chibisov, A. K.; Görner, H. J. Phys. Chem. A 1997, 101, 7304-7310.
8. Ponterini, G.; Momicchioli, F. Chem. Phys. 1991, 151, 111-126.
9. Rodriguez, J.; Scherlis, D.; Estrin, D.; Aramendia, P. F.; Negri, R. M. J. Phys. Chem. A 1997, 101, 6998-7006.
10. Sanchez-Galvez, A.; Hunt, P.; Robb, M. A.; Olivucci, M.; Vreven, T.; Schlegel, H. B. J. Am. Chem. Soc. 2000, 122, 2911-2924.
11. Smith, J. O.; Olson, D. A.; Armitage, B. A. J. Am. Chem. Soc. 1999, 121, 2686-2695.
12. Soper, S. A.; Mattingly, Q. L. J. Am: Chem. Soc. 1994, 116, 3744-3752.
13. Vaveliuk, P.; Scaffardi, L. B.; Duchowicz, R. J. Phys. Chem. 1996, 100, 11630-11635.
14. Wabuyele, M. B.; Farquar, H.; Stryjewski, W.; Hammer, R. P.; Soper, S. A.; Cheng, Y. W.; Barany, F. J. Am. Chem. Soc. 2003, 125, 6937-6945.
15. Hertz, A. H. Adv. Colloid Interface Sci. 1977, 8, 237-298.
16. Chatterjee, S.; Gottschalk, P.; Davis, P. D.; Schuster, G. B. J. Am. Chem. Soc. 1988, 110, 2326-2328.
17. Dragsten, P. R.; Webb, W. W. Biochemistry 1978, 17, 5228-5240.
18. Krieg, M.; Redmond, R. W. Photochem. Photobiol. 1993, 57, 472-479.
19. Benniston, A. C.; Harriman, A. J. Chem. Soc., Faraday Trans. 1998, 94, 1841-1847.
20. Benniston, A. C.; Harriman, A.; Mcavoy, C. J. Chem. Soc., Faraday Trans. 1998, 94, 519-525.
21. Shank, C. V.; Ippen, E. P. Dye Lasers; Springer-Verlag: Heidelberg, 1973.
22. Maeda, M. Laser Dyes; Academic Press: Tokyo, 1984.
23. Widengren, J.; Schwille, P. J. Phys. Chem. A 2000, 104, 6416-6428.
24. Köhn, F.; Hofkens, J.; Gronheid, R.; Van der Auweraer, M.; De Schryver, F. C. J. Phys. Chem. A 2002, 106, 4808-4814.
25. Weston, K. D.; Carson, P. J.; Metiu, H.; Buratto, S. K. J. Chem. Phys. 1998, 109, 7474-7485.
26. Widengren, J.; Seidel, C. A. M. Phys. Chem. Chem. Phys. 2000, 2, 3435-3441.
27. Tinnefeld, P.; Herten, D. P.; Sauer, M. J. Phys. Chem. A 2001, 105, 7989-8003.
28. Tinnefeld, P.; Buschmann, V.; Weston, K. D.; Sauer, M. J. Phys. Chem. A 2003, 107, 323-327.
29. Sahyun, M. R. V.; Serpone, N. J. Phys. Chem. A 1997, 101, 9877-9883.
30. Redmond, R. W.; Kochevar, I. E.; Krieg, M.; Smith, G.; McGimpsey, W. G. J. Phys. Chem. A 1997, 101, 2773-2777.
31. West, W.; Pearce, S.; Grum, F. J. Phys. Chem. 1967, 71, 1316-1326.
32. Baraldi, I.; Carnevali, A.; Momicchioli, F.; Ponterini, G. Spectrochim. Acta, Part A 1993, 49, 471-495.
33. English, D. S.; Harbron, E. J.; Barbara, P. F. J. Phys. Chem. A 2000, 104, 9057-9061.
34. Deniz, A. A.; Dahan, M.; Grunwell, J. R.; Ha, T.; Faulhaber, A. E.; Chemla, D. S.; Weiss, S.; Schultz, P. G. Proc. Natl. Acad. Sci. U.S.A. 1999, 96, 3670-3675.
35. Ha, T.; Ting, A. Y.; Liang, J.; Deniz, A. A.; Chemla, D. S.; Schultz, P. G.; Weiss, S. Chem. Phys. 1999, 247, 107-118.
36. Basché, T.; Kummer, S.; Brauchle, C. Nature 1995, 373, 132-134.
37. Moerner, W. E. J. Phys. Chem. B 2002, 106, 910-927.
38. Tamarat, P., Maali, A.; Lounis, B.; Orrit, M. J. Phys. Chem. A 2000, 104, 1-16.
39. Peterman, E. J. G.; Brasselet, S.; Moerner, W. E. J. Phys. Chem. A 1999, 103, 10553-10560.
40. Veerman, J. A.; Garcia-Parajo, M. F.; Kuipers, L.; van Hulst, N. F. Phys. Rev. Lett. 1999, 83, 2155-2158.
41. Hübner, C. G.; Zumofen, G.; Renn, A.; Herrmann, A.; Müllen, K. ; Basché, T. Phys. Rev. Lett. 2003, 91, 093903-1-4.
42. Lu, H. P.; Xie, X. S. Nature 1997, 143, 385-388.
43. Widengren, J.; Mets, Ülo; Rigler, R., Chem. Phys. 1999, 250, 171-186.
44. Ha, T. Methods 2001, 25, 78-86.
45. Huang, Z. X.; Ji, D. M.; Xia, A. D.; Koberling, F.; Patting, M.; Erdmann, R. J. Am. Chem. Soc. 2005, 127, 8064-8066.
46. Lu, H. P.; Xie, X. S. Nature 1997, 385, 143-146.
47. Lu, H. P.; Xie, X. S. J. Phys. Chem. B 1997, 101, 2753-2757.
48. Tinnefeld, P.; Buschmann, V.; Herten, D. P.; Han, K. T.; Sauer, M. Single Mol. 2000, 1, 215-223.
49. Ha, T.; Ting, A. Y.; Liang, J.; Brett Caldwell, W.; Deniz, A. A.; Chemla, D. S.; Schultz, P. G.; Weiss, S. Proc. Natl. Acad. Sci. U.S.A. 1999, 96, 893-898.
50. Grunwell, J. R.; Glass, J. L.; Lacoste, T. D.; Deniz, A. A.; Chemla, D. S.; Schultz, P. G. J. Am. Chem. Soc. 2001, 123, 4295-4303.
51. Fleury, L.; Segura, J. M.; Zumofen, G.; Hecht, B.; Wild, U. P. Phys. Rev. Lett. 2000, 84, 1148-1151.
52. Eggeling, C.; Widengren, J.; Rigler, R.; Seidel, C. A. M. Anal. Chem. 1998, 70, 2651-2659.
53. Strictly speaking, there is still a small possibility for the molecule to jump to the dim state during the long "on" state. The dim state (cis-Cy5) occurs in solution more frequently than on the dry glass surface. The on ↔ dim transition is a statistical process that happens on average after a certain number of photocycles. More on ↔ dim transitions will be expected with increasing excitation intensity because of the thermoinduced back-isomerization from cis to trans conformations; the saturated counts at higher excitation power as shown in Figure 3b may probably be due to more on ↔ dim transitions before photobleaching. From the laser photolysis results, the RISC could only happen in the trans isomer rather than in the cis isomer since the excited wavelength used for single molecule measurements is in resonance with the ground-state absorption and triplet state absorption of trans isomer. Therefore, to determine the RISC at single molecule levels, we must choose those single molecules on the glass with the more stable "on" state.
54. Reindl, S.; Penzkofer, A. Chem. Phys. 1996, 213, 429-438.
55. Larkin, J. M.; Donaldson, W. R.; Foster, T. H.; Knox, R. S. Chem. Phys. 1999, 244, 319-330.
56. Masuo, S.; Vosch, T.; Cotlet, M.; Tinnefeld, P.; Habuchi, S.; Bell, T. D. M.; Oesterling, I.; Beljonne, D.; Champagne, B.; Müllen, K.; Sauer, M.; Hofkens, J.; Schryver, F. C. D. J. Phys. Chem. B 2004, 108, 16686-16696.
57. Tinnefeld, P.; Hofkens, J.; Herten, D. P.; Masuo, S.; Vosch, T.; Cotlet, M.; Habuchi, S.; Müllen, K.; Schryver, F. C. D.; Sauer, M. ChemPhysChem 2004, 5, 1786-1790.