A bromoenol lactone suicide substrate inactivates group VIA phospholipase A2 by generating a diffiusible bromomethyl keto acid that alkylates cysteine thiols

Biochemistry

Volumn 45, Issue 3, 2006, Pages 1061-1073

  Song, Haowei ^a   Ramanadham, Sasanka ^a   Bao, Shunzhong ^a   Hsu, Fong Fu ^a   Turk, John ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALKYLATION; DIFFUSION; ESTERS; FATTY ACIDS; HYDROLYSIS; MASS SPECTROMETRY;

ACID HYDROLYSIS; BROMOENOL LACTONE; KETO ACID; PHOSPHOLIPASE;

PHOSPHOLIPIDS;

2 LYSOPHOSPHOLIPID; ARACHIDONIC ACID; BROMOENOL LACTONE; CYSTEINE DERIVATIVE; FATTY ACID; OXOACID; PHOSPHOLIPASE A2; PHOSPHOLIPID; SERINE; SUICIDE SUBSTRATE; SULFIDE; THIOL DERIVATIVE; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

ALKYLATION; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; COVALENT BOND; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME MODIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; HYDROLYSIS; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN FAMILY;

AMINO ACID SEQUENCE; BACULOVIRIDAE; CELLS, CULTURED; CYSTEINE; DIFFUSION; ENZYME INHIBITORS; HYDROCARBONS, BROMINATED; KETO ACIDS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NAPHTHALENES; PHOSPHOLIPASES A; PYRONES; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; SULFHYDRYL COMPOUNDS;

ANIMALIA;

EID: 31144458847     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi052065q     Document Type: Article

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