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Volumn 45, Issue 2, 2006, Pages 288-295

Cloning, expression, and purification of C-terminal quarter of the heavy chain of botulinum neurotoxin type A

Author keywords

Botulinum; Dot blot; Expression; Gangliosides; Neurotoxin; Purification; Receptor

Indexed keywords

BOTULINUM TOXIN A; GANGLIOSIDE; PEPTIDE FRAGMENT; RECOMBINANT PROTEIN; SYNAPTOTAGMIN;

EID: 31044444759     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.07.020     Document Type: Article
Times cited : (23)

References (26)
  • 1
    • 0033065263 scopus 로고    scopus 로고
    • Structure-function relationship of clostridial neurotoxins
    • L. Li, and B.R. Singh Structure-function relationship of clostridial neurotoxins J. Toxicol. 18 1999 95 112
    • (1999) J. Toxicol. , vol.18 , pp. 95-112
    • Li, L.1    Singh, B.R.2
  • 2
    • 0029950893 scopus 로고    scopus 로고
    • Critical aspects of bacterial protein toxins
    • B.R. Singh Critical aspects of bacterial protein toxins Adv. Exp. Med. Biol. 391 1996 63 84
    • (1996) Adv. Exp. Med. Biol. , vol.391 , pp. 63-84
    • Singh, B.R.1
  • 3
    • 0033887387 scopus 로고    scopus 로고
    • Intimate details of the most poisonous poison
    • B.R. Singh Intimate details of the most poisonous poison Nature. Struct. Biol. 7 2000 617 619
    • (2000) Nature. Struct. Biol. , vol.7 , pp. 617-619
    • Singh, B.R.1
  • 4
    • 0028310404 scopus 로고
    • Mechanism of action of tetanus and botulinum neurotoxins
    • C. Montecucco, and G. Schiavo Mechanism of action of tetanus and botulinum neurotoxins Microbiology 13 1994 1 8
    • (1994) Microbiology , vol.13 , pp. 1-8
    • Montecucco, C.1    Schiavo, G.2
  • 5
    • 46149134882 scopus 로고
    • How do tetanus and botulinum toxins bind to neuronal membrane
    • C. Montecucco How do tetanus and botulinum toxins bind to neuronal membrane Trends Biochem. Sci. 11 1986 314 317
    • (1986) Trends Biochem. Sci. , vol.11 , pp. 314-317
    • Montecucco, C.1
  • 6
    • 1242320307 scopus 로고    scopus 로고
    • CC-domain of botulinum neurotoxins a and B exhibits a singular ganglioside binding site displaying serotype specific carbohydrate interaction
    • CC-domain of botulinum neurotoxins A and B exhibits a singular ganglioside binding site displaying serotype specific carbohydrate interaction Mol. Microbiol. 51 2004 631 643
    • (2004) Mol. Microbiol. , vol.51 , pp. 631-643
    • Rummel, A.1    Mahrhold, S.2    Bigalke, H.3    Binz, T.4
  • 7
    • 0032515962 scopus 로고    scopus 로고
    • Role of zinc in the structure and toxic activity of botulinum neurotoxin
    • F. Fu, R. Lomneth, S. Cai, and B.R. Singh Role of zinc in the structure and toxic activity of botulinum neurotoxin Biochemistry 37 1998 5267 5278
    • (1998) Biochemistry , vol.37 , pp. 5267-5278
    • Fu, F.1    Lomneth, R.2    Cai, S.3    Singh, B.R.4
  • 8
    • 0028491306 scopus 로고
    • Clostridial neurotoxins as tools to investigate the molecular events of neurotransmitter release
    • G. Schiavo, O. Rossetto, and C. Montecucco Clostridial neurotoxins as tools to investigate the molecular events of neurotransmitter release Semin. Cell Biol. 5 1994 221 229
    • (1994) Semin. Cell Biol. , vol.5 , pp. 221-229
    • Schiavo, G.1    Rossetto, O.2    Montecucco, C.3
  • 10
    • 0032872478 scopus 로고    scopus 로고
    • Gangliosides are the binding substances in neural cells for tetanus and botulinum toxins in mice
    • M. Kitamura, K. Takamiya, S. Aizawa, K. Furukawa, and K. Furukawa Gangliosides are the binding substances in neural cells for tetanus and botulinum toxins in mice Biochim. Biophys. Acta 1441 1999 1 3
    • (1999) Biochim. Biophys. Acta , vol.1441 , pp. 1-3
    • Kitamura, M.1    Takamiya, K.2    Aizawa, S.3    Furukawa, K.4    Furukawa, K.5
  • 12
    • 0032189201 scopus 로고    scopus 로고
    • Isolation of synaptotagmin as a receptor for types a and e botulinum neurotoxin and analysis of their comparative binding using a new microtiter plate assay
    • L. Li, and B.R. Singh Isolation of synaptotagmin as a receptor for types A and E botulinum neurotoxin and analysis of their comparative binding using a new microtiter plate assay J. Nat. Toxins 7 1998 215 226
    • (1998) J. Nat. Toxins , vol.7 , pp. 215-226
    • Li, L.1    Singh, B.R.2
  • 13
    • 0034730834 scopus 로고    scopus 로고
    • Structure-based sequence alignment for the beta-trefoil subdomain of the clostridial neurotoxin family provides residue level information about the putative ganglioside binding site
    • K. Ginalski, C. Venclovas, B. Lesyng, and K. Fidelis Structure-based sequence alignment for the beta-trefoil subdomain of the clostridial neurotoxin family provides residue level information about the putative ganglioside binding site FEBS Lett. 482 2000 119 124
    • (2000) FEBS Lett. , vol.482 , pp. 119-124
    • Ginalski, K.1    Venclovas, C.2    Lesyng, B.3    Fidelis, K.4
  • 14
    • 0031712779 scopus 로고    scopus 로고
    • Crystal structure of botulinum neurotoxin type a and implications for toxicity
    • D.B. Lacy, W. Tepp, A.C. Cohen, B.R. DasGupta, and R.C. Stevens Crystal structure of botulinum neurotoxin type A and implications for toxicity Nat. Struct. Biol. 5 1998 898 902
    • (1998) Nat. Struct. Biol. , vol.5 , pp. 898-902
    • Lacy, D.B.1    Tepp, W.2    Cohen, A.C.3    Dasgupta, B.R.4    Stevens, R.C.5
  • 15
    • 0025314995 scopus 로고
    • The complete sequence of botulinum neurotoxin type a and comparison with other clostridial neurotoxins
    • T. Binz, H. Kurazono, M. Wille, J. Frevert, K. Wernars, and H. Neimann The complete sequence of botulinum neurotoxin type A and comparison with other clostridial neurotoxins J. Biol. Chem. 265 1990 9153 9158
    • (1990) J. Biol. Chem. , vol.265 , pp. 9153-9158
    • Binz, T.1    Kurazono, H.2    Wille, M.3    Frevert, J.4    Wernars, K.5    Neimann, H.6
  • 16
    • 0034708794 scopus 로고    scopus 로고
    • The structures of the H(C) fragment of tetanus toxin with carbohydrate subunit complexes provide insight into ganglioside binding
    • P. Emsley, C. Fotinou, I. Black, N.F. Fairweather, I.G. Charles, C. Watts, E. Hewitt, and N.W. Isaacs The structures of the H(C) fragment of tetanus toxin with carbohydrate subunit complexes provide insight into ganglioside binding J. Biol. Chem. 275 2000 8889 8894
    • (2000) J. Biol. Chem. , vol.275 , pp. 8889-8894
    • Emsley, P.1    Fotinou, C.2    Black, I.3    Fairweather, N.F.4    Charles, I.G.5    Watts, C.6    Hewitt, E.7    Isaacs, N.W.8
  • 18
    • 4644364793 scopus 로고    scopus 로고
    • Presynaptic receptor arrays for clostridial neurotoxins
    • C. Montecucco, O. Rossetto, and G. Schiavo Presynaptic receptor arrays for clostridial neurotoxins Trends Microbiol. 12 2004 442 446
    • (2004) Trends Microbiol. , vol.12 , pp. 442-446
    • Montecucco, C.1    Rossetto, O.2    Schiavo, G.3
  • 19
    • 0041652393 scopus 로고    scopus 로고
    • Vaccines for preventing botulism
    • M.F. Brin J. Jankovic M. Hallett Lippincott Williams and Wilkins Philadelphia
    • L.A. Smith, and M.P. Byrne Vaccines for preventing botulism M.F. Brin J. Jankovic M. Hallett Scientific and Therapeutic Aspects of Botulinum Toxin 2002 Lippincott Williams and Wilkins Philadelphia 427 437
    • (2002) Scientific and Therapeutic Aspects of Botulinum Toxin , pp. 427-437
    • Smith, L.A.1    Byrne, M.P.2
  • 20
    • 0033731911 scopus 로고    scopus 로고
    • Identification of the characteristics that underlie botulinum toxin potency: Implications for designing novel drugs
    • L.L. Simpson Identification of the characteristics that underlie botulinum toxin potency: implications for designing novel drugs Biochimie 82 2000 943 953
    • (2000) Biochimie , vol.82 , pp. 943-953
    • Simpson, L.L.1
  • 21
    • 0031772527 scopus 로고    scopus 로고
    • Development of recombinant vaccines for botulinum neurotoxin
    • L.A. Smith Development of recombinant vaccines for botulinum neurotoxin Toxicon 36 1998 539 1548
    • (1998) Toxicon , vol.36 , pp. 539-1548
    • Smith, L.A.1
  • 22
    • 4143073791 scopus 로고    scopus 로고
    • Interaction between the two subdomains of the C-terminal part of the botulinum neurotoxin a is essential for the generation of protective antibodies
    • M. Tavallaie, A. Chenal, D. Gillet, Y. Pereira, M. Manich, M. Gibert, S. Raffestin, M.R. Popoff, and J.C. Marvaud Interaction between the two subdomains of the C-terminal part of the botulinum neurotoxin A is essential for the generation of protective antibodies FEBS Lett. 572 2004 299 306
    • (2004) FEBS Lett. , vol.572 , pp. 299-306
    • Tavallaie, M.1    Chenal, A.2    Gillet, D.3    Pereira, Y.4    Manich, M.5    Gibert, M.6    Raffestin, S.7    Popoff, M.R.8    Marvaud, J.C.9
  • 23
    • 53149099323 scopus 로고    scopus 로고
    • In vitro translation of type a clostridium botulinum neurotoxin heavy chain and analysis of its binding to rat synaptosomes
    • L. Li, and B.R. Singh In vitro translation of type A clostridium botulinum neurotoxin heavy chain and analysis of its binding to rat synaptosomes J. Protein Chem. 18 1999 89 95
    • (1999) J. Protein Chem. , vol.18 , pp. 89-95
    • Li, L.1    Singh, B.R.2
  • 24
    • 0019293885 scopus 로고
    • An absolute method for protein determination based on difference in absorbance at 235 and 280 nm
    • J.R. Whitaker, and P.E. Granum An absolute method for protein determination based on difference in absorbance at 235 and 280 nm Anal. Biochem. 109 1980 156 159
    • (1980) Anal. Biochem. , vol.109 , pp. 156-159
    • Whitaker, J.R.1    Granum, P.E.2
  • 25
    • 0033602922 scopus 로고    scopus 로고
    • Enhancement of the endopeptidase activity of botulinum neurotoxin by its associated proteins and dithiothreitol
    • S. Cai, H.K. Sarkar, and B.R. Singh Enhancement of the endopeptidase activity of botulinum neurotoxin by its associated proteins and dithiothreitol Biochemistry 38 1999 6903 6910
    • (1999) Biochemistry , vol.38 , pp. 6903-6910
    • Cai, S.1    Sarkar, H.K.2    Singh, B.R.3
  • 26
    • 1442289359 scopus 로고    scopus 로고
    • Cloning, high level expression, single step purification and binding activity of His6-tagged recombinant type B Botulinum neurotoxin heavy chain transmembrane and binding domain
    • Y. Zhou, and B.R. Singh Cloning, high level expression, single step purification and binding activity of His6-tagged recombinant type B Botulinum neurotoxin heavy chain transmembrane and binding domain Protein Expr. Purif. 34 2004 8 16
    • (2004) Protein Expr. Purif. , vol.34 , pp. 8-16
    • Zhou, Y.1    Singh, B.R.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.