Comet assay comparison of different Corbicula fluminea (Mollusca) tissues for the detection of genotoxicity

Genetics and Molecular Biology

Volumn 28, Issue 3, 2005, Pages 464-468

  Rigonato, Janaina ^a   Mantovani, Mário Sérgio ^a   Jordão, Berenice Quinzani ^a  

^a STATE UNIVERSITY OF LONDRINA   (Brazil)

Author keywords

Biomonitoring; Comet assay; Corbicula fluminea; Digestive gland; Gills; Hemolymph

Indexed keywords

MESYLIC ACID METHYL ESTER; MUTAGENIC AGENT; TOXIN;

ANIMAL TISSUE; AQUATIC ENVIRONMENT; ARTICLE; CELL SUSPENSION; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; ENVIRONMENTAL MONITORING; EXOCRINE GLAND; EXPOSURE; GENOTOXICITY; GILL; MOLLUSC; NONHUMAN; REGRESSION ANALYSIS; WATER CONTAMINATION; WATER POLLUTANT;

ANIMALIA; BIVALVIA; CORBICULA FLUMINEA; MOLLUSCA;

EID: 30544454677     PISSN: 14154757     EISSN: 16784685     Source Type: Journal    
DOI: 10.1590/S1415-47572005000300023     Document Type: Article

References (21)

1. Bilos C, Colombo JC, Presa MJR (1998) Trace metals in suspended particles, sediments and Asiatic clams (Corbicula fluminea) of the Río de la Plata Estuary, Argentina. Environ Pollution 99:1-11.
2. Dowling K and Mothersill C (2001) The further development of rainbow trout primary epithelial cell cultures as a diagnostic tool in ecogenotoxicology risk assessment. Aquatic Toxicol 53:279-289.
3. Farbain DW, Olive PL and O'Neil KL (1995) The comet assay: A comprehensive review. Mutat Res 339:37-59.
4. Johns C and Luoma SN (1990) Arsenic in benthic bivalves of San Francisco Bay and the Sacramento/San Joaquin River delta, The Science of the Total Environ 97/98:673-684.
5. Klaude M, Eriksson S, Nygren J and Ahnström G (1996) The comet assay: Mechanisms and technical considerations. Mutat Res 363:89-96.
6. Klobuèar GIV, Pavlica M, Erben R and Pape D (2003) Application of the micronucleus and comet assays to mussel Dreissena polymorpha haemocytes for genotoxicity monitoring of freshwater environments. Aquatic Toxicol 64:15-23.
7. Le Pennec G and Le Pennec M (2001) Evaluation of the toxicity of chemical compounds using digestive acini of the bivalve mollusk Pecten maximus L. maintained alive in vitro. Aquat Toxicol 53:1-7.
8. Lee RF and Steinert S (2003) Use of the single cell gel electrophoresis/ comet assay for detecting DNA damage in aquatic (marine and freshwater) animals. Mutat Res 544:43-64.
9. López-Barea J and Pueyo C (1998) Mutagen content and metabolic activation of promutagens by molluscs as biomarkers of marine pollution. Mutat Res 399:3-15.
10. Mitchelmore CL and Chipman JK (1998) DNA strand breakage in aquatic organisms and the potential values of the comet assay in environmental monitoring. Mutat Res 399:135-147.
11. Mitchelmore CL, Birmelin C, Livingstone DR and Chipman JK (1998) Detection of DNA strand breaks in isolated mussel (Mytilus edulis L.) digestive gland cells using the "comet" assay. Ecotoxicol Environ Saf 41:51-58.
12. Nacci DF, Cayula S and Jackim F (1996) Detection of DNA damage in individual cells from marine organisms using the single cell gel assay. Aquatic Toxicol 35:197-210.
13. Narbonne JF, Djomo JE, Ribeira, FV and Garrigues P (1999) Accumulation kinetics of polycyclic aromatic hydrocarbons adsorbed to sediment by the mollusk Corbicula fluminea. Ecotoxicol Environ Saf 42:1-8.
14. Pavlica M, Klobuèar GIV, Moja N, Erben R and Pape D (2001) Detection of DNA damage in haemocytes of zebra mussel using comet assay. Mutat Res.490:209-214.
15. Pruski AM and Dixon DR (2002) Effects of cadmium on nuclear integrity and DNA repair efficiency in the gill cells of Mytilus edulis L. Aquatic Toxicol 57:127-137.
16. Rank J and Jensen K (2003) Comet assay on gill cells and hemocytes from the blue mussel Mitylus edulis. Ecotoxicol Environ Saf 54:323-329.
17. Roberts CA (1996) Selenium contamination in Corbicula transplanted into agricultural drains in the Imperial Valley, California, Division of Environ. Contaminants, Carsbad Field Office U.S. Fish and Wildlife Service, August.
18. Speit G and Hartmann A (1999) The comet assay (single-cell gel test). In Henderson DS (ed) Methods in Molecular Biology, v.113, DNA Repair Protocols: Eukaryotic Systems. Humana Press, Totowa, pp 203-212.
19. Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC and Sasaki YF (2000) Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing. Environ Molec Mutagenesis 35:206-221.
20. Venier P, Maron S and Canova S (1997) Detection of micronuclei in gill cells and haemocytes of mussels exposed to benzo[a]pyrene. Mutat Res 390:33-44.
21. Wilson JT, Pascoe PL, Parry JM and Dixon DR (1998) Evaluation of comet assay as a method for the detection of DNA damage in cells of a marine invertebrate, Mytilus edulis L. (Mollusca, Pelecipoda). Mutat Res 399:87-95.