Application of the micronucleus and comet assays to mussel Dreissena polymorpha haemocytes for genotoxicity monitoring of freshwater environments

Aquatic Toxicology

Volumn 64, Issue 1, 2003, Pages 15-23

  Klobučar, G öran I V ^a   Pavlica, Mirjana ^a   Erben, Radovan ^a   Papeš, Dražena ^a  

^a UNIVERSITY OF ZAGREB   (Croatia)

Author keywords

Comet assay; Dreissena polymorpha; Haemocytes; Micronucleus assay; Zebra mussel

Indexed keywords

FRESHWATER ENVIRONMENT; GENOTOXICITY; MOLLUSC; POLLUTION MONITORING;

ANIMAL CELL; ARTICLE; BLOOD CELL; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; DREISSENA POLYMORPHA; FRESHWATER ENVIRONMENT; GENOTOXICITY; MICRONUCLEUS; MUSSEL; NONHUMAN; POLLUTION; PRIORITY JOURNAL; RIVER ECOSYSTEM; ANIMAL; BIVALVE; COMPARATIVE STUDY; DOSE RESPONSE; DRUG EFFECT; ENVIRONMENTAL EXPOSURE; ENVIRONMENTAL MONITORING; GENETICS; METHODOLOGY; MICRONUCLEUS TEST; STATISTICS; WATER POLLUTANT;

BIVALVIA; DREISSENA; DREISSENA POLYMORPHA;

FRESH WATER;

ANIMALS; BIVALVIA; COMET ASSAY; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, DRUG; ENVIRONMENTAL EXPOSURE; ENVIRONMENTAL MONITORING; FRESH WATER; HEMOCYTES; MICRONUCLEUS TESTS; STATISTICS; WATER POLLUTANTS;

EID: 0043022216     PISSN: 0166445X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-445X(03)00009-2     Document Type: Article

References (52)

1. Al-Sabti K. Handbook of Genotoxic Effects and Fish Chromosomes. 1991;Krištoff, Ljubljana, Slovenia.
2. Belpaeme K., Delbeke K., Zhu L., Kirchvolders M. Cytogenetic studies of brown trout (Salmo trutta fario) using the micronucleus test and the alkaline comet assay. Mutagenesis. 11:1996;485-492.
3. Black M.C., Ferrel J.R., Horning R.C., Martin L.K. DNA strand breakage in freshwater mussels (Anodonta grandis) exposed to lead in the laboratory and field. Environ. Toxicol. Chem. 15:1996;802-808.
4. Bolognesi C., Landini E., Roggieri P., Fabbri R., Viarengo A. Genotoxicity biomarkers in the assessment of heavy metal effects in mussels: experimental studies. Environ. Mol. Mutagen. 33:1999;287-292.
5. Bombail V., Aw D., Gordon E., Batty J. Application of the comet and micronucleus assays to butterfish (Pholis gunnellus) erythrocytes from the Firth of Forth, Scotland. Chemosphere. 44:2001;383-392.
6. Bolognesi C., Rabboni R., Malfatto C., Roggieri P. Application of biological markers in aquatic organisms as indicators of marine pollution. Mar. Environ. Res. 39:1995;345-356.
7. Burgeot T., His E., Galgani F. The micronucleus assay in Crassostrea gigas for the detection of seawater genotoxicity. Mutat. Res. 342:1995;125-140.
8. Cotelle S., Férard J.F. Comet assay in genetic ecotoxicology: a review. Environ. Mol. Mutagen. 34:1999;246-255.
9. Dennog C., Hartmann A., Frey G., Speit G. Detection of DNA damage after hyperbaric oxygen (HBO) therapy. Mutagenesis. 11:1996;605-609.
10. Deventer K. Detection of genotoxic effects on cells of liver and gills of B. rerio by means of single cell gel electrophoresis. Bull. Environ. Contam. Toxicol. 56:1996;911-918.
11. Depledge M.H. The ecotoxical significance of genotoxicity in marine invertebrates. Mutat. Res. 399:1998;109-122.
12. Devaux A., Flammarion P., Bernardon V., Garric J., Monod G. Monitoring of the chemical pollution of the river Rhône through measurement of DNA damage and cytochrome P4501A induction in Chub (Leuciscus cephalus). Mar. Environ. Res. 46:1998;257-262.
13. Dopp E., Barker C.M., Schiffmann D., Reinisch C.L. Detection of micronuclei in hemocytes of Mya arenaria: association with leukemia and induction with an alkylating agent. Aquat. Toxicol. 34:1996;31-45.
14. Fairbairn D.W., Olive P.L., O'Neil K.L. The comet assay: a comprehensive review. Mutat. Res. 339:1995;37-59.
15. Frenzilli G., Nigro M., Scarcelli V., Gorbi S., Regoli F. DNA integrity and total oxyradical scavenging capacity in the Mediterranean mussel, Mytilus galloprovincialis: a field study in a highly eutrophicated coastal lagoon. Aquat. Toxicol. 53:2001;19-32.
16. Grisolia C.K., Starling F.L.R.M. Micronuclei monitoring of fishes from Lake Paranoá, under influence of sewage treatment plant discharges. Mutat. Res. 491:2001;39-44.
17. Guecheva T., Henriques J.A.P., Erdtmann B. Genotoxic effects of copper sulphate in freshwater planarian in vivo, studied with the single-cell gell test (comet assay). Mutat. Res. 497:2001;19-27.
18. Hartmann A., Plappert U., Raddatz K., Grünert-Fuchs M., Speit G. Does physical activity induce DNA damage? Mutagenesis. 9:1994;269-272.
19. Hartmann A., Speit G. The contribution of cytotoxicity to DNA-effects in the single cell gell test (comet assay). Toxicol. Lett. 90:1997;183-188.
20. Hartmann A., Elhajouji A., Kiskinis E., Poetter F., Martus H.-J., Fjällman A., Frieauff W., Suter W. Use of the alkaline assay for industrial genotoxicity screening: comparative investigation with the micronucleus test. Food Chem. Toxicol. 39:2001;843-858.
21. He J.L., Chen W.L., Jin L.F., Jin H.Y. Comparative evaluation of the in vitro micronucleus test and the comet assay for the detection of genotoxic effects of X-ray radiation. Mutat. Res. 469:2000;223-231.
22. Helma C., Uhl M. A public domain image analysis program for the single cell gell electrophoresis (comet) assay. Mutat. Res. 466:2000;9-15.
23. Henderson L., Albertini S., Aardema M. Thresholds in genotoxicity responses. Mutat. Res. 464:2000;123-128.
24. Jaylet A., Deparis P., Ferrier V., Grinfeld S., Siboulet R. A new micronucleus test using peripheral blood erythrocytes of the new Pleurodeles waltl to detect mutagen in freshwater pollution. Mutat. Res. 164:1986;245-257.
25. Kammann U., Riggers J.C., Theobald N., Steinhart H. Genotoxic potential of marine sediments from the North Sea. Mutat. Res. 467:2000;161-168.
26. Kirsch-Volders M., Sofuni T., Aaderma M., Albertini S., Eastmond D., Fenech M., Ishidate M., Lorge E., Norppa H., Surallés J., Von der Hude W., Wakata A. Report from the in vitro micronucleus assay working group. Environ. Mol. Mutagen. 35:2000;167-172.
27. Kurelec B. The genotoxic disease syndrome. Mar. Environ. Res. 35:1993;341-348.
28. Majone F., Brunetti R., Gola I., Levis A.G. Persistence of micronuclei in the marine mussel, Mytillus galoprovincialis, after treatment with mitomycin C. Mutat. Res. 191:1987;157-161.
29. Majone F., Beltrame C., Brunetti R. Frequencies of micronuclei detected on Mytillus galloprovincialis by different staining techniques after treatment with zinc chloride. Mutat. Res. 209:1988;131-134.
30. Mersch J., Beauvais M.-N., Nagel P. Induction of micronuclei in haemocytes and gill cells of zebra mussels, Dreissena polymorpha, exposed to clastogens. Mutat. Res. 371:1996;47-55.
31. Mersch J., Beauvais M.-N. The micronucleus assay in the zebra mussel, Dreissena polymorpha, to in situ monitor genotoxicity in freshwater environments. Mutat. Res. 393:1997;141-149.
32. Mitchelmore C.L., Chipman J.K. DNA strand breakage in aquatic organisms and the potential value of the comet assay in environmental monitoring. Mutat. Res. 399:1998;135-147.
33. Nacci D.E., Cayula S., Jackim E. Detection of DNA damage in individual cells from marine organisms using the single cell gell assay. Aquat. Toxicol. 35:1996;197-210.
34. Pandrangi R., Petras M., Ralph S., Vrzoc M. Alkaline single cell gell (comet) assay and genotoxicity monitoring using bullheads and carp. Environ. Mol. Mutagen. 26:1995;345-356.
35. Pavlica M., Klobučar G.I.V., Vetma N., Erben R., Papeš D. Detection of micronuclei in haemocytes of zebra mussel and great ramshorn snail exposed to pentachlorophenol. Mutat. Res. 465:2000;145-150.
36. Pavlica M., Klobučar G.I.V., Mojaš N., Erben R., Papeš D. Detection of DNA damage in haemocytes of zebra mussel using comet assay. Mutat. Res. 490:2001;209-214.
37. Ralph S., Petras M., Pandrangi R., Vrzoc M. Alkaline single-cell gell (comet) assay and genotoxicity monitoring using two species of tadpoles. Environ. Mol. Mutagen. 28:1996;112-120.
38. Ralph S., Petras M. Genotoxicity monitoring of small bodies of water using two species of tadpoles and the alkaline single cell gell (comet) assay. Environ. Mol. Mutagen. 29:1997;418-430.
39. Ralph S., Petras M. Caged amphibian tadpoles and in situ genotoxicity monitoring of aquatic environments with the alkaline single cell gel electrophoresis (comet) assay. Mutat. Res. 413:1998;235-250.
40. Robbiano L., Carrozzino R., Porta Puglia C., Corbu C., Brambilla G. Correlation between induction of DNA fragmentation and micronuclei formation in kidney cells from rats and humans and tissue-specific carcinogenetic activity. Toxicol. Appl. Pharmacol. 161:1999;153-159.
41. Rodríguez-Ariza A., Abril N., Navas J.I., Dorado G., López-Barea J., Pueyo C. Metal, mutagenicity, and biochemical studies on bivalve molluscs from Spanish coasts. Environ. Mol. Mutagen. 19:1992;112-124.
42. Rojas E., Lopez M.C., Valverde M. Single cell gel electrophoresis assay: methodology and applications. J. Chromatogr. B. 722:1999;225-254.
43. Sánchez-Galán S., Linde A.R., Izquierdo J.I., García-Vázquez E. Micronuclei and fluctuating asymmetry in brown trout (Salmo trutta): complementary methods to biomonitor freshwater ecosystems. Mutat. Res. 412:1998;219-225.
44. Shaw J.P., Large A.T., Chipman J.K., Livingstone D.R., Peters L.D. Seasonal variation in mussel Mytilus edulis digestive gland cytochrome P4501A- and 2E-immunoidentified protein levels and DNA strand breaks (Comet assay). Mar. Environ. Res. 50:2000;405-409.
45. Singh N.P., Tice R.R., Schneider E.L. A simple technique for quantitation of low levels of damage in individual cells. Exp. Cell Res. 175:1988;184-191.
46. Steinert S.A., Streib-Montee R., Leather J.M., Chadwick D.B. DNA damage in mussels at sites in San Diego Bay. Mutat. Res. 399:1998;65-85.
47. Steinert S.A. DNA damage as bivalve biomarker. Biomarkers. 4:1999;492-496.
48. Šalagovič J., Gilles J., Verschaeve L., Kalina I. The comet assay for the detection of genotoxic damage in the earthworms: a promising tool for assessing the biological hazards of polluted sites. Folia Biologica (Praha). 42:1996;17-21.
49. van Hummelen P., Zoll C., Paulussen J., Kirsch-Volders M., Jaylet A. The micronucleus test in Xenopus: a new and simple in vivo technique for detection of mutagens in fresh water. Mutagenesis. 4:1989;12-16.
50. Venier P., Maron S., Canova S. Detection of micronuclei in gill cells and hamocytes of mussels exposed to benzo[a]pyrene. Mutat. Res. 390:1997;33-44.
51. Vrzoc M., Petras M.L. Comparison of alkaline single cell gell (comet) and peripheral blood micronucleus assays in detecting DNA damage caused by direct and indirect acting mutagens. Mutat. Res. 381:1997;31-40.
52. Wilson J.T., Pascoe P.L., Parry J.M., Dixon D.R. Evaluation of the comet assay as a method for the detection of DNA damage in the cells of a marine invertebrate, Mytillus edulis L. (Mollusca: Pelecypoda). Mutat. Res. 399:1998;87-95.