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•] present another piece of evidence that supports the guard hypothesis. RIN4 is shown to interact with RPS2 (a CC-NBS-LRR protein) in planta. RPS2 is able to detect the presence of the type III effector AvrRpt2. Interestingly, the sequence of AvrRpt2 is not related to that of AvrRpm1 or AvrB, and AvrRpt2 induces the disappearance of RIN4.
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HSP90 is identified as a RAR1 and SGT1 interactor. The authors also show, through gene silencing, that HSP90 is required for N gene function.
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Liu Y., Burch-Smith T., Schiff M., Feng S., Dinesh-Kumar S.P. Molecular chaperone Hsp90 associates with resistance protein N and its signaling proteins SGT1 and Rar1 to modulate an innate immune response in plants. J Biol Chem. 3:2004;2101-2108 HSP90 is identified as a RAR1 and SGT1 interactor. The authors also show, through gene silencing, that HSP90 is required for N gene function.
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Lu R., Malcuit T., Moffett P., Ruiz M.T., Peart J., Wu A.J., Rathjen J.P., Bendahmane A., Day L., Baulcombe D.C. High-throughput virus-induced gene silencing implicates heat shock protein 90 in plant disease resistance. EMBO J. 21:2003;5690-5699 HSP90 is identified by reverse genetics using a virus-induced gene silencing (VIGS) screen. The authors demonstrate the involvement of HSP90 in both Pto (Resistance to P. syringae pv. tomato) function and Rx-mediated resistance.
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