Evidence that catalytically-inactivated thrombin forms non-covalently linked dimers that bridge between fibrin/fibrinogen fibers and enhance fibrin polymerization

Biophysical Chemistry

Volumn 110, Issue 1-2, 2004, Pages 93-100

  Mosesson, M W ^a   Hernandez, I ^a   Siebenlist, K R ^a  

^a BLOOD RESEARCH INSTITUTE   (United States)

Author keywords

Exosite; Fibrin; Fibrin(ogen), a solution containing both fibrin and fibrinogen; Fibrinogen; FPR thr, phe pro arg thrombin; FPR thr, FPRck inactivated thrombin; FPRck, phe pro arg chloromethyl ketone; Thrombin

Indexed keywords

FIBRIN; FIBRINOGEN; PROTEIN DERIVATIVE;

ARTICLE; BINDING AFFINITY; BINDING SITE; CATALYSIS; CATALYST; CONCENTRATION RESPONSE; COVALENT BOND; CROSS LINKING; DIMERIZATION; MOLECULAR DYNAMICS; MOLECULAR INTERACTION; MOLECULAR MECHANICS; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN BINDING; REACTION ANALYSIS;

AMINO ACID CHLOROMETHYL KETONES; BINDING SITES; BIOPOLYMERS; CATALYSIS; DIMERIZATION; FIBRIN; FIBRINOGEN; PROTEIN BINDING; THROMBIN; TIME FACTORS;

EID: 3042692360     PISSN: 03014622     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bpc.2004.01.007     Document Type: Article

References (37)

1. Kaminski M., McDonagh J. Enhancement of fibrin polymerization by active site-inhibited thrombin. Henschen A., Hessel B., McDonagh J., Saldeen T.G. Fibrinogen. Structural Variants and Interactions. 1985;51-63 Walter de Gruyter, Berlin
2. Kaminski M., Siebenlist K.R., Mosesson M.W. Evidence for thrombin enhancement of fibrin polymerization that is independent of its catalytic activity. J. Lab. Clin. Med. 117:1991;218-225
3. Liu C.Y., Nossel H.Y., Kaplan K.L. The binding of thrombin by fibrin. J. Biol. Chem. 254:1979;10 421-10 425
4. Berliner L.J., Sugawara Y., Fenton J.W.I. Human α-thrombin binding to non-polymerized fibrin-sepharose: evidence for an anionic binding region. Biochemistry. 24:1985;7005-7009
5. Kaminski M., McDonagh J. Inhibited thrombins. Interactions with fibrinogen and fibrin. Biochem. J. 242:1987;881-887
6. Fenton J.W. II, Olson T.A., Zabinski M.P., Wilner G.D. Anion-binding exosite of human α-thrombin and fibrin(ogen) recognition. Biochemistry. 27:1988;7106-7112
7. Wilner G.D., Danitz M.P., Mudd M.S. Selective immobilization of α-thrombin by surface-bound fibrin. J. Lab. Clin. Med. 1981;403-411
8. Meh D.A., Siebenlist K.R., Mosesson M.W. Identification and characterization of the thrombin binding sites on fibrin. J. Biol. Chem. 271:1996;23121-23125
9. Bode W., Mayr I., Baumann U., Huber R., Stone S.R., Hofsteenge J. The refined 1.9 Å crystal structure of human α-thrombin: Interaction with D-Phe-Pro-Arg chloromethylketone and significance of the Tyr-Pro-Pro-Trp insertion segment. EMBO. J. 8:1989;3467-3475
10. Bode W., Turk D., Karshikov A. The refined 1.9 Å X-ray crystal structure of D-Phe-Pro-Arg chloromethylketone-inhibited human α-thrombin. Protein. Sci. 1:1992;426-471
11. Tsiang M., Jain A.K., Dunn K.E., Rojas M.E., Leung L.L., Gibbs C.S. Functional mapping of the surface residues of human thrombin. J. Biol. Chem. 270:1995;16854-16863
12. Hall S.W., Gibbs C.S., Leung L.L.K. Identification of critical residues on thrombin mediating its interaction with fibrin. Thromb. Haemost. 86:2001;1466-1474
13. Lovely R.S., Moaddel M., Farre D.H. II. Fibrinogen γ′ chain binds thrombin exosite II. J. Thromb. Haemostas. 1:2003;124-131
14. Pospisil C.H., Stafford A.R., Fredenburgh J.C., Weitz J.I. Evidence that both exosites on thrombin participate in its high affinity interaction with fibrin. J. Biol. Chem. 278:2003;21584-21591
15. Celikel R., McClintock R.A., Roberts J.R., Mendolicchio G.L., Ware J., Varughese K.I., Ruggeri Z.M. Modulation of alpha-thrombin function by distinct interactions with platelet glycoprotein Ibalpha. Science. 301:2003;218-221
16. Dumas J.J., Kumar R., Seehra J., Somers W.S., Mosyak L. Crystal structure of the GpIb alpha-thrombin complex essential for platelet aggregation. Science. 301:2003;222-226
17. Siebenlist K.R., Meh D.A., Mosesson M.W. Plasma factor XIII binds specifically to fibrinogen molecules containing γ′ chains. Biochemistry. 35:1996;10448-10453
18. Pandya B.V., Gabriel J.L., O'Brien J., Budzynski A.Z. Polymerization site in the γ chain of fibrin: mapping of the Bβ1-55 sequence. Biochemistry. 30:1991;162-168
19. Bock L.C., Griffin L.C., Latham J.A., Vermaas E.H., Toole J.J. Selection of single-stranded DNA molecules that bind and inhibit human thrombin. Nature. 355:1992;564-566
20. I. Pechik, J. Madrazo, M.W. Mosesson, I. Hernandez, G.L. Gilliland, L. Medved, Crystal structure of the complex between thrombin and the central'E' region of fibrin, Proc. Natl. Acad. Sci. USA (in press) (2004)
21. Mosesson M.W., Finlayson J.S., Umfleet R.A. Human fibrinogen heterogeneities. III. Identification of γ chain variants. J. Biol. Chem. 247:1972;5223-5227
22. Wolfenstein-Todel C., Mosesson M.W. Human plasma fibrinogen heterogeneity: evidence for an extended carboxyl-terminal sequence in a normal gamma chain variant (γ′). Proc. Natl. Acad. Sci.USA. 77:1980;5069-5073
23. Cooper A.V., Standeven K.F., Ariens R.A. Fibrinogen gamma-chain splice variant γ′ alters fibrin formation and structure. Blood. 102:2003;535-540
24. Meh D.A., Mosesson M.W., Siebenlist K.R., Simpson-Haidaris P.J., Brennan S.O., DiOrio J.P., et al. Fibrinogen Naples I (Bβ A68T) non-substrate thrombin binding capacities. Thromb. Res. 103:2001;63-73
25. Stubbs M.T., Bode W. A player of many parts: the spotlight falls on thrombin's structure. Thromb. Res. 69:1993;1-58
26. Binnie C.G., Lord S.T. The fibrinogen sequences that interact with thrombin. Blood. 81:1993;3186-3192
27. Bing D.H., Cory M., Fenton J.W. II. Exosite affinity labeling of human thrombins; similar labeling on the A chain and B chain fragments of clotting α- and non-clotting γ/β thrombins. J. Biol. Chem. 252:1977;8027-8034
28. Boissel J.P., LeBonniec B., Rabiet M.-J., Labie D., Elion J. Covalent structure of β and γ autolytic derivatives of human α-thrombin. J. Biol. Chem. 259:1984;5691-5697
29. Rydel T.J., Yin M., Padmanabhan K.P., Blankenship D.T., Cardin A.D., Correa P.E., et al. Crystallographic structure of human γ-thrombin. J. Biol. Chem. 269:1994;22000-22006
30. Siebenlist K.R., DiOrio J.P., Budzynski A.Z., Mosesson M.W. The polymerization and thrombin-binding properties of des-(B beta 1-42)- fibrin. J. Biol. Chem. 265:1990;18650-18655
31. Pandya B.V., Cierniewski C.S., Budzynski A.Z. Conservation of human fibrinogen conformation after cleavage of the Bβ chain NH2-terminus. J. Biol. Chem. 260:1985;2994-3000
32. Kaminski M., McDonagh J. Studies on the mechanism of thrombin interaction with fibrin. J. Biol. Chem. 258:1983;10530-10535
33. Naski M.C., Shafer J.A. thrombin-catalyzed hydrolysis of fibrin I. J. Biol. Chem. 265:1990;1401-1407
34. Hopfner K.P., Di Cera E. Energetics of thrombin-fibrinogen interaction. Biochemistry. 31:1992;11567-11571
35. Hosokawa K., Ohnishi T., Shima M., Nagata M., Koide T. Preparation of anhydrothrombin and characterization of its interaction with natural thrombin substrates. Biochem. J. 354:2001;309-313
36. Bernocco S., Ferri F., Profumo A., Cuniberti C., Rocco M. Polymerization of rod-like macromolecular monomers studied by stopped- flow, multiangle light scattering: set-up, data processing, and application to fibrin formation. Biophys. J. 79:2000;561-583
37. Rocco M., Bernocco S., Turci M., Profumo A., Cuniberti C., Ferri F. Early events in the polymerization of fibrin. Ann. NY. Acad. Sci. 936:2001;167-184