Crystal structure of the Gplbα-thrombin complex essential for platelet aggregation

Science

Volumn 301, Issue 5630, 2003, Pages 222-226

  Dumas, John J ^a   Kumar, Ravindra ^a   Seehra, Jasbir ^a   Somers, William S ^a   Mosyak, Lidia ^a  

^a Wyeth ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESION; BLOOD VESSELS; BONDING; COMPLEXATION; CRYSTAL STRUCTURE; CYTOLOGY; DRUG PRODUCTS; PATHOLOGY;

PLATELETS;

PROTEINS;

GLYCOPROTEIN; GLYCOPROTEIN IB ALPHA; THROMBIN; UNCLASSIFIED DRUG; THROMBIN RECEPTOR;

CRYSTAL STRUCTURE; PATHOLOGY;

ARTICLE; BINDING SITE; COMPLEX FORMATION; CRYSTAL STRUCTURE; DRUG DESIGN; MOLECULAR BIOLOGY; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; THROMBOCYTE AGGREGATION; CHEMICAL STRUCTURE; CHEMISTRY; CRYSTALLIZATION; HUMAN; HYDROGEN BOND; HYDROPHOBICITY; METABOLISM; PHYSIOLOGY; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; THROMBOCYTE; THROMBOCYTE ADHESIVENESS; X RAY CRYSTALLOGRAPHY;

BINDING SITES; BLOOD PLATELETS; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; HUMANS; HYDROGEN BONDING; HYDROPHOBICITY; MODELS, MOLECULAR; PLATELET ADHESIVENESS; PLATELET AGGREGATION; PLATELET GLYCOPROTEIN GPIB-IX COMPLEX; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; THROMBIN;

EID: 0038157329     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1083917     Document Type: Article

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44. We thank E. Presman for growth of CHO cells, T. McDonagh for assistance with Gplbα purification, J. Rouse for mass spectrometry, G. Shaw for comments on the manuscript, and J. Bard and G. McDermott for the assistance with data collection. The coordinates for the Gplbα-thrombin complex have been deposited in the Protein Data Bank with the accession code 1P8V.