Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions

BMC Biotechnology

Volumn 3, Issue , 2003, Pages

  Ponchel, Frederique ^a   Toomes, Carmel ^a   Bransfield, Kieran ^a   Leong, Fong T ^a   Douglas, Susan H ^a   Field, Sarah L ^a   Bell, Sandra M ^a   Combaret, Valerie ^b   Puisieux, Alain ^b   Mighell, Alan J ^a   Robinson, Philip A ^a   Inglehearn, Chris F ^a   Isaacs, John D ^a   Markham, Alex F ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

^b CENTRE LÉON BÉRARD   (France)

Author keywords

Amplification; Deletion; Real time PCR; Rearrangement; SYBR green

Indexed keywords

FLUORESCENT DYE; MYC PROTEIN; T LYMPHOCYTE RECEPTOR; DNA; GLI PROTEIN; GUANOSINE TRIPHOSPHATASE; LYMPHOCYTE ANTIGEN RECEPTOR; ONCOPROTEIN; OPA1 PROTEIN, HUMAN; ORGANIC COMPOUND; SYBR GREEN I; TRANSACTIVATOR PROTEIN; TRANSCRIPTION FACTOR;

ANALYTIC METHOD; ARTICLE; ASSAY; AUTOSOMAL DOMINANT OPTIC ATROPHY; BINDING AFFINITY; CELL LINE; CLINICAL PRACTICE; CONTROLLED STUDY; COST; DIAGNOSTIC ACCURACY; EQUIPMENT DESIGN; FLUORESCENCE IN SITU HYBRIDIZATION; GENE AMPLIFICATION; GENE DELETION; GENE REARRANGEMENT; HUMAN; HUMAN CELL; HUMAN TISSUE; INTERMETHOD COMPARISON; NUCLEOTIDE SEQUENCE; PERIPHERAL BLOOD MONONUCLEAR CELL; PROCESS OPTIMIZATION; PUBLICATION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; SOUTHERN BLOTTING; TUMOR BIOPSY; CHEMISTRY; FEMALE; FLUORESCENCE; GENETICS; MALE; METHODOLOGY; OPTIC NERVE ATROPHY; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; TUMOR CELL LINE;

CELL LINE, TUMOR; DNA; FEMALE; FLUORESCENCE; GENE AMPLIFICATION; GENE DELETION; GENE REARRANGEMENT; GTP PHOSPHOHYDROLASES; HUMANS; MALE; ONCOGENE PROTEINS; OPTIC ATROPHY; ORGANIC CHEMICALS; POLYMERASE CHAIN REACTION; RECEPTORS, ANTIGEN, T-CELL; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TRANS-ACTIVATORS; TRANSCRIPTION FACTORS;

EID: 3042604472     PISSN: 14726750     EISSN: None     Source Type: Journal    
DOI: 10.1186/1472-6750-3-18     Document Type: Article

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