DNA-PK: The major target for wortmannin-mediated radiosensitization by the inhibition of DSB repair via NHEJ pathway

Journal of Radiation Research

Volumn 44, Issue 2, 2003, Pages 151-159

  Hashimoto, Mitsumasa ^a   Rao, Satish ^a,b   Tokuno, Osamu ^a   Yamamoto, Ken Ichi ^c   Takata, Minoru ^d   Takeda, Shunichi ^e   Utsumi, Hiroshi ^a  

^a KYOTO UNIVERSITY   (Japan)

^b KASTURBA MEDICAL COLLEGE   (India)

^c KANAZAWA UNIVERSITY   (Japan)

^d KAWASAKI MEDICAL SCHOOL   (Japan)

^e KYOTO UNIVERSITY FACULTY OF MEDICINE   (Japan)

Author keywords

Chicken knockout cells; DNA PK; Radiosensitization ATM; SCID cells; Wortmannin

Indexed keywords

CELL CULTURE; CELLS; MAMMALS; PROTEINS; REPAIR;

CATALYTIC SUBUNITS; CHICKEN KNOCKOUT CELL; DNA-DEPENDENT PROTEIN KINASE; DOUBLE STRAND BREAK REPAIR; MAMMARY CARCINOMA CELL; POST TREATMENT; RADIO-SENSITIZATION; RADIOSENSITIZATION ATM; SCID CELL; WORTMANNIN;

DNA;

DNA DEPENDENT PROTEIN KINASE; DOUBLE STRANDED DNA; RADIOSENSITIZING AGENT; WORTMANNIN;

ANIMAL CELL; ARTICLE; ATAXIA TELANGIECTASIA; CLONOGENIC ASSAY; CONTROLLED STUDY; DNA STRAND BREAKAGE; DRUG EFFECT; DRUG MECHANISM; ENZYME ACTIVITY; ENZYME INHIBITION; HUMAN; HUMAN CELL; NONHUMAN; RADIOSENSITIVITY; RADIOSENSITIZATION; SCID MOUSE;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS; GALLUS GALLUS;

EID: 2942612900     PISSN: 04493060     EISSN: None     Source Type: Journal    
DOI: 10.1269/jrr.44.151     Document Type: Article

References (42)

1. Iliakis, G., Mehta, R. and Jackson, M. (1992) Level of double-strand break rejoining in Chinese hamster xrs-5 cells is dose-dependent implications for the mechanism of radiosensitivity. Int. J. Radiat. Biol. 61: 315-321.
2. Ward, J. F. (1994) The complexity of DNA damage: Relevance to biological consequences. Int. J. Radiat. Biol. 66: 427-432.
3. Kemp, L. M., Sedgwick, S. G. and Jeggo, P. A. (1984) X-ray sensitive mutants of Chinese hamster ovary cells defective in double strand break rejoining. Mutat. Res. 132: 189-196.
4. Giaccia, A. J., Denko, N., MacLaren, R., Mirman, D., Waldren, C., Hart, I. and Stamato T. D. (1990) Human chromosome 5 complements the DNA double-strand break-repair deficiency and gamma-ray sensitivity of the XR-1 hamster variant. Am. J. Hum. Genet. 47: 459-469.
5. Biedermann, K. A., Sun, J., Giaccia, A. J., Tosto, L. M. and Brown, J. M. (1991) Scid mutation in mice confers hypersensitivity to ionizing radiation and a deficiency in DNA double strand break repair. Proc. Natl. Accad. Sci. USA 88: 1394-1397.
6. Hendrickson, E. A., Qin, X., Bump, E. A., Schatz, D. G., Oettinger, M. and Weaver, D. T. (1991) A link between double-strand break related repair and V (D)J recombination: The scid mutation. Proc. Natl. Acad. Sci. USA 88: 4061-4065.
7. Okayasu, R., Suetomi, K. and Ullrich, R. L. (1998) Wortmannin inhibits repair of DNA double-strand breaks in irradiated normal human cells. Radiat. Res. 149: 440-445.
8. Chernikova, S. B., Wells, R. L. and Elkind, M. M. (1999) Wortmannin sensitizes mammalian cells to radiation by inhibiting the DNA-dependent protein kinase-mediated rejoining of double-strand breaks. Radiat Res. 151: 159-166.
9. Arcaro, A. and Wymann, M. P. (1993) Wortmannin is a potent phosphatidylinositol 3 kinase inhibitor: the role of phosphatidylinositol 3,4,5 -triphosphate in neutrophil responses. Biocherm. J. 296: 297-301.
10. Powis, G., Bonjouklian, R., Berggren, M. M., Gallegos, A., Abraham, R., Ashendel, C., Zalkow , L., Matter, W. F., Dodge, J. and Grindey, G. (1994) Wortmannin, a potent and selective inhibitor of phosphatidylinositol 3-kinase. Cancer Res. 54: 2419-2423.
11. Okada, T., Sakuma, L., Fukui, Y., Hazeki, O. and Ui, M. (1994) Blockage of chemotactic peptide induced stimulation of neutrophils by wortmannin as a result of selective inhibition of phosphatidylinositol 3-kinase. J. Biol. Chem. 269: 3563-3567.
12. Price, B. D. and Youmell, M. B. (1996) The phosphatidylinositol 3-kinase inhibitor wortmannin sensitizes murine fibroblasts and human tumor cells to radiation and blocks induction of p53 following DNA damage. Cancer Res. 56: 246-250.
13. Kubota, N., Ozawa, F., Okada, S., Inada, T., Komatsu, K. and Okayasu, R. (1998) The phosphatidylinositol 3-kinase inhibitor wortmannin sensitizes quiescent but not proliferating MG-63 human osteosarcoma cells to radiation. Cancer Lett. 133: 161-167.
14. Rozenzweig, K. E., Youmell, M. B., Palayoor, S. T. and Price, B. D. (1997) Radiosensitization of human tumor cells by the phosphatidylinositol 3-kinase inhibitors wortmannin and LY294002 correlates with inhibition of DNA-dependent protein kinase and prolonged G2-M delay. Cli. Cancer Res. 3: 1149-1156.
15. Sarkaria, J. N., Tibbetts, R. S., Busby, E. C., Kennedy, A. P., Hill, D. E. and Abraham, R. T. (1998) Inhibition of phosphoinositide 3-kinase related kinases by the radiosensitizing agent wortmannin. Cancer Res. 58: 4375-4382.
16. Tomita, M., Suzuki, N., Matsumoto, Y., Hirano, K., Umeda, N. and Sakai, K. (2000) Sensitization by wortmannin of heat- or X-ray induced cell death in cultured Chinese hamster V79 Cells. J. Radiat. Res. 41: 93-102.
17. Blunt, T., Finnie, N. J., Taccioli, G. E., Smith, G. C., Demengeot, J., Gottlieb, T. M., Mizuta, R., Varghese, A. J., Alt F. W. and Jeggo, P. A. (1995) Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation. Cell 80: 813-823.
18. Jackson, S. P. and Jeggo, P. A. (1996) DNA-double strand breaks. Crit. Rev. Euk. Gene Expression. 6: 345-375.
19. Jeggo, P. A. (1997) DNA-PK: At the cross-roads of biochemistry and genetics. Mutat. Res. 384: 1-14.
20. Keith, C. T. and Schreiber, S. L. (1995) PIK-related kinases: DNA repair, recombination, and cell cycle checkpoints. Science 270: 50-51.
21. Savitsky, K., Bar-Shira, A., Gilad, S., Rotman, G., Ziv, Y., Vanagaite, L., Tagle, D. A., Smith, S., Uziel, T., Sfez, S., Ashkenazi, M., Pecker, I., Frydman, M., Harnik ,R., Patanjali, S. R., Simmons, A., Clines, G. A., Sartiel, A., Gatti, R. A., Chessa, L., Sanal, O., Lavin, M. F., Jaspers, N. G. J., Taylor, A. M. R., Arlett C. F., Miki, T., Weissman, S. M., Lovett, M., Collins, F. S. and Shiloh, Y. (1995) A single ataxia telangiectasia gene with a product similar to PI-3 kinase. Sceince 268: 1749-1753.
22. Meyn, M. S. (1995) Ataxia telangiectasia and cellular responses to DNA damage. Cancer Res. 55: 5991-6001.
23. Foray, N., Priestley, A., Alsbeih, G., Badie, C., Capulas, E. P., Arlett, C. F. and Malaise, E. P. (1997) Hypersensitiviey of ataxia telangiectasia fibroblasts to ionizing radiation is associated with a repair deficiency of DNA double-strand breaks. Int. J. Radiat. Biol. 72: 271-283.
24. Takata, M., Sasaki, M.S., Sonoda, E., Morrison, C., Hashimoto, M., Utsumi, H., Yamaguchi-Iwai, Y., Shinohara, A. and Takeda, S. (1998) Homologous recombination and non-homologous end-joining pathways of DNA double strand break repair have overlapping roles in the maintenance of chromosomal integrity in vertebrate cells. EMBO J. 17: 5497-5508.
25. Utsumi, H., Tano, K., Takata, M., Takeda, S. and Elkind, M. M. (2001) Requirement for repair of DNA double-strand breaks by-homologous recombination in split-dose recovery. Radiat. Res. 155, 680-686.
26. Utsumi, H. and Elkind, M. M. (1985) Two forms of potentially lethal damage have similar repair kinetics in plateau- and in log-phase cells. Int. J. Radiat. Biol. 47, 569-580.
27. Komatsu, K., Ohta, T., Jinno, Y., Niikawa, N. and Okumura, Y. (1993) Functional complementation in mouse-human radiation hybrids assigns the putative murine scid gene to the pericentric region of human chromosome 8. Human Mol. Genetics 2: 1031-1034.
28. Fukumura, R., Araki, R., Fujimori, A., Mori, M., Saito, T., Watanabe, F., Sarashi, M., Itsukaichi, H., Eguchi-Kasai, K., Sato, K., Tatsumi, K. and Abe, M. (1998) Murine cell line SX9 bearing a mutation in the dna-pkcs gene exhibits aberrant V(D)J recombination not only in the coding joint but also in the signal joint. J. Biol. Chem. 273: 13058-13064.
29. Fukushima, T., Takata, M., Morrison, C., Araki, R., Fujimori, A., Abe, M., Tatsumi, K., Jasin, M., Dhar, P. K., Sonoda, E., Chiba, T. and Takeda, S. (2001) Genetic analysis of the DNA-dependent protein kinase reveals an inhibitory role of Ku in late S-G2 phase DNA double-strand break repair. J. Biol. Chem. 276: 44413-44418.
30. Morrison, C., Sonoda, E., Takao, N., Shinohara, A., Yamamoto, K. and Takeda, S. (2000) The controlling role of ATM in homologous recombinational repair of DNA damage. EMBO J. 19: 463-471.
31. Boulton, S., Kyle, S., Yalcintepe, L. and Durkacz, B. W. (1996) Wortmannin is a potent inhibitor of DNA double strand break but not single strand break repair in Chinese hamster ovary cells. Carcinogenesis 17: 2285-2290.
32. DiBiase, S. J., Zeng, Z.-C., Chen, R., Hyslop, T., Curran Jr, W. J. and Iliakis, G. (2000) DNA-dependent protein kinase stimulates an independently active, nonhomologous, end-joining apparatus. Cancer Res. 60: 1245-1253.
33. Kirchgessner, C. U., Patil, C. K., Evans, J. W., Cuomo, C. A., Fried, L. M., Carter, T., Oettinger, M. A. and Brown, J. M. (1995) DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect. Science 267: 1178-1183.
34. Lieber, M. R., Hesse, J. E., Lewis, S., Bosma, G. C., Rosenberg, N., Mizuuchi, K., Bosma, M. J. and Gellert, M. (1988) The defect in murine severe combined immune deficiency: Joining of signal sequences but not coding segments in V(D)J recombination. Cell 55: 7-16.
35. Taghian, D. G. and Nickoloff, J. A. (1997) Chromosomal double-strand breaks induce gene conversion at high frequency in mammalian cells. Mol. Cell. Biol. 17: 6386-6393.
36. Jin, S. and Weaver, D. T. (1997) Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions. EMBO J. 16: 6874-6885.
37. Hartley, K. O., Gell, D., Smith, G. C. M., Zhang, H., Divecha, N., Connelly, M. A., Admon, A., Lees-Miller, S. P., Anderson, C. W., and Jackson, S. P. (1995) DNA-dependent protein kinase catalytic subunit: A relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product. Cell 82: 849-856.
38. Yoshida, M., Hosoi, Y., Miyachi, H., Ishii, N., Matsumoto, Y., Enomoto, A., Nakagawa, K., Yamada, S., Suzuki, N. and Ono, T. (2002) Roles of DNA-dependent protein kinase and ATM in cell-cycle-dependent radiation sensitivity in human cells. Int. J. Radiat. Biol. 78: 503-512.
39. Chaung, W., Mi, L. J. and Boorstein, R. J. (1997) The p53 status of Chinese hamster V79 cells frequently used for studies on DNA damage and DNA repair. Nucleic Acids Res. 25: 992-994.
40. Kastan, M. B., Zhan, Q., el-Deiry, W. S., Carrier, F., Jacks, T., Walsh, W., Plunkett, B. S., Vogelstein, B. and Fornace Jr, A. J. (1992) A mammalian cell cycle check point pathway utilizing p53 and gadd45 is defective in ataxia telangiectasia. Cell 71: 587-597.
41. Khanna, K. K., Beamish, H., Yan, J., Hobson, K., Williams, R., Dunn, I. and Lavin, M. F. (1995) Nature of G1-S cell cycle checkpoint defect in ataxia-telangiectasia. Oncogene 11: 609-618.
42. Sonoda, E., Sasaki, M. S., Buerstedde, J. M., Bezzubova, O., Shinohara, A., Ogawa, H., Takata, M., Yamaguchi-Iwai, Y. and Takeda, S. (1998) Rad51-deficient vertebrate cells accumulate chromosomal breaks prior to cell death. EMBO J. 17: 598-608.