Improved indicators for assessing the reliability of detection and quantification by kinetic PCR

Clinical Chemistry

Volumn 49, Issue 6, 2003, Pages 973-976

  Soong, Richie ^a   Ladányi, András ^b  

^a University of Alabama at Birmingham   (United States)

^b DANA FARBER CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYTIC METHOD; ARTICLE; MEASUREMENT; NUCLEIC ACID ANALYSIS; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; RELIABILITY; REPRODUCIBILITY;

BETA 2-MICROGLOBULIN; COLORECTAL NEOPLASMS; HUMANS; HYDROXYMETHYLBILANE SYNTHASE; INTERMEDIATE FILAMENT PROTEINS; KERATIN-20; KINETICS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TUMOR MARKERS, BIOLOGICAL;

EID: 0037683339     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/49.6.973     Document Type: Article

References (22)

1. Freeman WM, Walker SJ, Vrana KE. Quantitative RT-PCR: pitfalls and potential. Biotechniques 1999;26:112-22,124-5.
2. Bustin SA. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 2000;25:169-93.
3. Bernard PS, Wittwer CT. Real-time PCR technology for cancer diagnostics. Clin Chem 2002;48:1178-85.
4. Stenman J, Orpana A. Accuracy in amplification. Nat Biotechnol 2001;19:1011-2.
5. Peccoud J, Jacob C. Theoretical uncertainty of measurements using quantitative polymerase chain reaction. Biophys J 1996;71:101-8.
6. Taberlet P, Griffin S, Goossens B, Questiau S, Manceau V, Escaravage N, et al. Reliable genotyping of samples with very low DNA quantities using PCR. Nucleic Acids Res 1996;24:3189-94.
7. Karrer EE, Lincoln JE, Hoganhout S, Bennett AB, Bostock RM, Martineau B, et al. In situ isolation of mRNA from individual plant cells: creation of cell-specific cDNA libraries. Proc Natl Acad Sci U S A 1995;92:3814-8.
8. Morrison TB, Weis JJ, Wittwer CT. Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification. Biotechniques 1998;24:954-8,960,962.
9. Schmitt Y. Performance characteristics of quantification assays for human immunodeficiency virus type 1 RNA. J Clin Virol 2001;20:31-3.
10. Cavrois M, Wain-Hobson S, Wattel E. Stochastic events in the amplification of HTLV-I integration sites by linker-mediated PCR. Res Virol 1995;146:179-84.
11. Jung R, Ahmad-Nejad P, Wimmer M, Gerhard M, Wagener C, Neumaier M. Quality management and influential factors for the detection of single metastatic cancer cells by reverse transcriptase polymerase chain reaction. Eur J Clin Chem Clin Biochem 1997;35:3-10.
12. Melo JV, Yan XH, Diamond J, Lin F, Cross NC, Goldman JM. Reverse transcription/polymerase chain reaction (RT/PCR) amplification of very small numbers of transcripts: the risk in misinterpreting negative results. Leukemia 1996;10:1217-21.
13. Lion T. Current recommendations for positive controls in RT-PCR assays. Leukemia 2001;15:1033-7.
14. Higuchi R, Fockler C, Dollinger G, Watson R. Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Biotechnology 1993;11:1026-30.
15. Heid CA, Stevens J, Livak KJ, Williams PM. Real time quantitative PCR. Genome Res 1996;6:986-94.
16. Soong R, Beyser K, Basten O, Kalbe A, Rueschoff J, Tabiti K. Quantitative reverse transcription-polymerase chain reaction detection of cytokeratin 20 in noncolorectal lymph nodes. Clin Cancer Res 2001;7:3423-9.
17. T) method. Methods 2001;25:402-8.
18. Wolfel T, Hauer M, Klehmann E, Brichard V, Ackermann B, Knuth A, et al. Analysis of antigens recognized on human melanoma cells by A2-restricted cytolytic T lymphocytes (CTL). Int J Cancer 1993;55:237-44.
19. Zaaijer HL, Cuypers HT, Reesink HW, Winkel IN, Gerken G, Lelie PN. Reliability of polymerase chain reaction for detection of hepatitis C virus. Lancet 1993;341:722-4.
20. Noordhoek GT, van Embden JD, Kolk AH. Reliability of nucleic acid amplification for detection of Mycobacterium tuberculosis: an international collaborative quality control study among 30 laboratories. J Clin Microbiol 1996;34:2522-5.
21. Keilholz U, Willhauck M, Rimoldi D, Brasseur F, Dummer W, Rass K, et al. Reliability of reverse transcription-polymerase chain reaction (RT-PCR)-based assays for the detection of circulating tumour cells: a quality-assurance initiative of the EORTC Melanoma Cooperative Group. Eur J Cancer 1998;34:750-3.
22. Pritzker KP. Cancer biomarkers: easier said than done. Clin Chem 2002;48:1147-50.