Cell surface display of lipase in Pseudomonas putida KT2442 using OprF as an anchoring motif and its biocatalytic applications

Applied and Environmental Microbiology

Volumn 71, Issue 12, 2005, Pages 8581-8586

  Lee, Seung Hwan ^a   Lee, Sang Yup ^a   Park, Byoung Chul ^b  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE; CELL MEMBRANES; ENZYME KINETICS; FLUORESCENCE; GENES; IMMUNOLOGY; PROTEINS;

BLOT ANALYSIS; CELL SURFACE DISPLAY; LIPASE GENE; MEMBRANE PROTEIN;

ENZYMES;

ORGANIC SOLVENT; OUTER MEMBRANE PROTEIN; OUTER MEMBRANE PROTEIN OPRF; RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

ENZYME;

ANALYTIC METHOD; ARTICLE; BACTERIAL GENE; BACTERIUM CULTURE; BIOCATALYST; CARBOXY TERMINAL SEQUENCE; CELL SURFACE; CHIRALITY; CONTROLLED STUDY; ENANTIOMER; ENANTIOSELECTIVITY; ENZYME ACTIVITY; FUSION GENE; IMMUNOFLUORESCENCE MICROSCOPY; MOLECULAR CLONING; NONHUMAN; OPRF GENE; PROTEIN MOTIF; PSEUDOMONAS PUTIDA; SIK W1 LIPASE GENE; WESTERN BLOTTING;

CATALYSIS; CELL MEMBRANE; FLOW CYTOMETRY; KINETICS; LIPASE; PLASMIDS; PORINS; PSEUDOMONAS PUTIDA; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS FLUORESCENS; PSEUDOMONAS PUTIDA;

EID: 29144489184     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.12.8581-8586.2005     Document Type: Article

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