Preparation of lipases for use in organic solvents

Enzyme and Microbial Technology

Volumn 31, Issue 6, 2002, Pages 833-841

  Persson, Mattias ^a   Mladenoska, Irina ^a   Wehtje, Ernst ^a   Adlercreutz, Patrick ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

Accurel EP 100; Crown ether; Immobilisation; Lipase; Potassium chloride; Sol gel; Surfactant coating

Indexed keywords

ADSORPTION; CROWN ETHERS; ENZYME IMMOBILIZATION; MICROORGANISMS; ORGANIC SOLVENTS; SOL-GELS; SURFACE ACTIVE AGENTS;

BUFFER SOLUTIONS;

ENZYMES;

BIOSURFACTANT; BUFFER; CROWN ETHER; GUANINE NUCLEOTIDE BINDING PROTEIN; HEXANE; ORGANIC SOLVENT; POLYPROPYLENE; POTASSIUM CHLORIDE; SORBITAN STEARATE; TRIACYLGLYCEROL LIPASE;

ADSORPTION; ARTICLE; BURKHOLDERIA CEPACIA; CANDIDA RUGOSA; CATALYSIS; CULTURE MEDIUM; DISPERSION; ENZYME ISOLATION; FREEZING; GAS CHROMATOGRAPHY; GEL; IMMOBILIZATION; MOLECULAR INTERACTION; NONHUMAN; POWDER; RHIZOMUCOR; RHIZOMUCOR MIEHEI; SOLUBILITY;

BURKHOLDERIA CEPACIA; CANDIDA; CANDIDA RUGOSA; HUMICOLA; PSEUDOMONAS; RHIZOMUCOR; RHIZOMUCOR MIEHEI; THERMOMYCES LANUGINOSUS;

EID: 0036843107     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(02)00184-9     Document Type: Article

References (36)

1. Schmitke J., Wescott C., Klibanov A. The mechanistic dissection of the plunge in enzymatic activity upon transition from water to anhydrous solvents. J. Am. Chem. Soc. 118:1995;3360-3365.
2. Larsson K., Janssen A., Adlercreutz P., Mattiasson B. Three systems used for biocatalysis in organic solvents. A comparative study. Biocatalysis. 4:1990;163-175.
3. Martinek K., Klyachko N., Kabanov A., Khmelnitsky Y., Levashov A. Micellar enzymology: its relation to membranology. Biochim. Biophys. Acta. 981:1989;161-172.
4. Inada Y., Takahashi K., Yoshimoto T., Ajima A., Matsushima A., Saito Y. Application of polyethylene glycol-modified enzymes in biotechnological processes organic solvent-soluble enzymes. Trends Biotechnol. 11:1986;270-275.
5. Koops B.C., Verheij M.H., Slotboom A.J., Egmond M.R. Effect of chemical modification on the activity of lipases in organic solvents. Enzyme Microb. Technol. 25:1999;622-631.
6. Otamiri M., Adlercreutz P., Mattiasson B. Complex formation between chymotrypsin and ethyl cellulose as a means to solubilise the enzyme in active form in toluene. Biocatalysis. 6:1992;291-305.
7. Okahata Y., Ijiro K. A lipid-coated lipase as new catalyst for triglyceride synthesis in organic solvents. J. Chem. Soc. Chem. Commun. 20:1988;1392-1394.
8. Gitlesen T., Bauer M., Adlercreutz P. Adsorption of lipase on polypropylene powder. Biochim. Biophys. Acta. 1345:1997;188-196.
9. Persson M., Wehtje E., Adlercreutz P. Immobilisation of lipases by adsorption and deposition: high protein loading gives lower water activity optimum. Biotechnol. Lett. 22:2000;1571-1575.
10. Reetz M., Zonta A., Simpelkamp J. Efficient immobilisation of lipases by entrapment in hydrophobic sol-gel materials. Biotechnol. Bioeng. 49:1996;527-534.
11. Khmelnitsky Y., Welch S., Clark D., Dordick J.S. Salts dramatically enhance activity of enzymes suspended in organic solvents. J. Am. Chem. Soc. 116:1994;2647-2648.
12. Ru M.T., Dordick J.S., Reimer J.A., Clark D. Optimizing the salt-induced activation of enzymes in organic solvents. Biotechnol. Bioeng. 63:1999;233-241.
13. Broos J., Sakodinskaya I.K., Engberseen J.F.J., Verboom W., Reinhoudt D.N. Large activation of serine proteases by pretreatment with crown ethers. J. Chem. Soc. Chem. Commun. 2:1995;255-256.
14. Van Unen D.J., Engbersen J.F.J., Reinhoudt D.N. Large acceleration of α-chymotrypsin-catalyzed dipeptide formation by 18-crown-6 in organic solvents. Biotechnol. Bioeng. 59:1998;553-556.
15. Triantafyllou A., Wehtje E., Adlercreutz P., Mattiasson B. How do additives affect enzyme activity and stability in nonaqueous media? Biotechnol. Bioeng. 54:1997;67-76.
16. Secundo F., Carrea G., Soregaroli C., Varinelli D., Morrone R. Activity of different Candida antarctica lipase B formulations in organic solvents. Biotechnol. Bioeng. 73:2001;157-163.
17. Dabulis K., Klibanov A. Dramatic enhancement of enzymic activity in organic solvents by lyoprotectants. Biotechnol. Bioeng. 41:1993;566-571.
18. Blinkovsky A. Method for solubilizing proteins in organic solvents. PCT Int Appl 1997, 19.
19. Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
20. Gotham S.M., Fryer P.J., Paterson W. The measurement of insoluble proteins using a modified Bradford assay. Anal. Biochem. 173:1988;353-358.
21. Okahata Y., Mori T. Lipid-coated enzymes as efficient catalysts in organic media. Trends Biotechnol. 15:1997;50-54.
22. Basheer S., Mogi K., Nakajima M. Surfactant-modified lipase for the catalysis of the interesterification of triglycerides and fatty acids. Biotechnol. Bioeng. 45:1995;187-195.
23. Kamiya N., Murakami E., Goto M., Nakashio F. Effect of using a co-solvent in the preparation of surfactant-coated lipases on catalytic activity in organic media. J. Ferment. Bioeng. 82:1996;37-41.
24. Kamiya N., Goto M., Nakashio F. Surfactant-coated lipase suitable for the enzymatic resolution of menthol as a biocatalyst in organic media. Biotechnol. Prog. 11:1995;270-275.
25. Huang S.Y., Chang H.L., Goto M. Preparation of surfactant-coated lipase for the esterification of geraniol and acetic acid in organic solvent. Enzyme Microb. Technol. 22:1998;552-557.
26. Goto M., Kamiya N., Miyata M., Nakashio F. Enzymatic esterification by surfactant-coated lipase in organic media. Biotechnol. Prog. 10:1994;263-268.
27. Sugimura Y., Fukunaga K., Matsuno T., Nakao K., Goto M., Nakashio F. A study on the surface hydrophobicity of lipases. Biochem. Eng. J. 5:2000;123-128.
28. Sugimura Y., Fukunaga K., Matsuno T., Nakao K., Goto M., Nakashio F. A study on the lipid-coating of lipases. Biochem. Eng. J. 2:1998;137-141.
29. Bosley J., Peilow A. Immobilisation of lipases on porous polypropylene: reduction in esterification efficiency at low loading. J. Am. Oil. Chem. Soc. 74:1997;107-111.
30. Ru M.T., Hirokane S., Lo A., Dordick J.S., Reimer J.A., Clark D. On the salt-induced activation of lyophilized enzymes in organic solvents: effect of salt kosmotropicity on enzyme activity. J. Am. Chem. Soc. 122:2000;1565-1571.
31. Engbersen J.F.J., Broos J., Verboom W., Reinhoudt D.N. Effects of crown ethers and small amounts of cosolvent on the activity and enantioselectivity of α-chymotrypsin in organic solvents. Pure Appl. Chem. 68:1996;2171-2178.
32. Gradillas A., Del Campo C., Sinisterra J.V., Llama E. Alteration of the reaction rate in the esterification of (R, S)-ibuprofen by addition of crown ether or porphyrin. Biotechnol. Lett. 18:1996;85-90.
33. Broos J., Visser A., Engbersen J.F.J., Verboom W., Van Hoek A., Reinhoudt D.N. Flexibility of enzymes suspended in organic solvents probed by time-resolved fluorescence anisotropy. Evidence that enzyme activity and enantioselectivity are directly related to enzyme flexibility. J. Am. Chem. Soc. 117:1995;12657-12663.
34. Rees D., Halling P. Chemical modification probes accessibility to organic phase: proteins on surfaces are more exposed than in lyophilized powders. Enzyme Microb. Technol. 28:2001;282-292.
35. Van Unen D.J., Engbersen J.F.J., Reinhoudt D.N. Studies on the mechanism of crown-ether-induced activation of enzymes in non-aqueous media. J. Mol. Catal. B: Enzymatic. 11:2001;877-882.
36. Santos A.M., Vidal M., Pacheco Y., Frontera J., Baez C., Ornellas O.et al. Effect of crown ethers on structure, stability, activity and enantioselectivity of subtilisin carlsberg in organic solvents. Biotechnol. Bioeng. 74:2001;295-308.