Directed evolution of bacterial alanine racemases with higher expression level

Journal of Bioscience and Bioengineering

Volumn 100, Issue 3, 2005, Pages 246-254

  Ju, Jiansong ^a   Misono, Haruo ^b   Ohnishi, Kouhei ^b  

^a EHIME UNIVERSITY   (Japan)

^b KOCHI UNIVERSITY   (Japan)

Author keywords

Alanine racemase; Catalytic activity; DNA shuffling; Enzyme stability; Serine

Indexed keywords

CATALYSIS; DNA; ENZYMES; ESCHERICHIA COLI; PROTEINS;

BACTERIAL ALANINE RACEMASES; ENZYME STABILITY; SERINE;

BACTERIA;

ALANINE RACEMASE; BACTERIAL ENZYME;

ARTICLE; AUXOTROPHY; CATALYSIS; CLONE; CONTROLLED STUDY; DNA SEQUENCE; DNA SHUFFLING; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE ISOLATION; GENE LIBRARY; GENETIC SCREENING; MOLECULAR EVOLUTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; SALMONELLA TYPHIMURIUM;

ALANINE RACEMASE; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BIOTECHNOLOGY; CATALYSIS; CLONING, MOLECULAR; DIRECTED MOLECULAR EVOLUTION; DNA SHUFFLING; ESCHERICHIA COLI; GENE LIBRARY; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; SALMONELLA TYPHIMURIUM; SERINE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; SALMONELLA TYPHIMURIUM;

EID: 28744447567     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.100.246     Document Type: Article

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