Genotyping of transgenic mice: Old principles and recent developments

Analytical Biochemistry

Volumn 344, Issue 1, 2005, Pages 1-7

  Schneider, Marlon R ^a   Wolf, Eckhard ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

GENOTYPING; TRANSGENIC MICE;

MAMMALS;

GENOMIC DNA;

CHROMOSOME REARRANGEMENT; DNA DETERMINATION; DOT HYBRIDIZATION; GENE IDENTIFICATION; GENE NUMBER; GENE TARGETING; GENOTYPE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REVIEW; SOUTHERN BLOTTING; TANDEM REPEAT;

MUS MUSCULUS;

EID: 27644547743     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.03.035     Document Type: Review

References (69)

1. R.D. Palmiter, R.L. Brinster, Germ-line transformation of mice, Annu. Rev. Genet. 20 (1986) 465-499.
2. R. Jaenisch, Transgenic animals, Science 240 (1988) 1468-1474.
3. D. Hanahan, Transgenic mice as probes into complex systems, Science 246 (1989) 1265-1275.
4. M.R. Capecchi, Altering the genome by homologous recombination, Science 244 (1989) 1288-1292.
5. P. Soriano, R.D. Cone, R.C. Mulligan, R. Jaenisch, Tissue-specific and ectopic expression of genes introduced into transgenic mice by retroviruses, Science 234 (1986) 1409-1413.
6. C. Lois, E.J. Hong, S. Pease, E.J. Brown, D. Baltimore, Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors, Science 295 (2002) 868-872.
7. A. Pfeifer, M. Ikawa, Y. Dayn, I.M. Verma, Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos, Proc. Natl. Acad. Sci. USA 99 (2002) 2140-2145.
8. A.C. Perry, T. Wakayama, H. Kishikawa, T. Kasai, M. Okabe, Y. Toyoda, R. Yanagimachi, Mammalian transgenesis by intracytoplasmic sperm injection, Science 284 (1999) 1180-1183.
9. R.D. Palmiter, Transgenic mice: the early days, Int. J. Dev. Biol. 42 (1998) 847-854.
10. R.K. Saiki, D.H. Gelfand, S. Stoffel, S.J. Scharf, R. Higuchi, G.T. Horn, K.B. Mullis, H.A. Erlich, Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase, Science 239 (1988) 487-491.
11. C. Abbott, S. Povey, N. Vivian, R. Lovell-Badge, PCR as a rapid screening method for transgenic mice, Trends Genet. 4 (1988) 325.
12. CA. Walter, D. Nasr-Schirf, V.J. Luna, Identification of transgenic mice carrying the CAT gene with PCR amplification, BioTechniques 7 (1989) 1065-1070.
13. C.S. Lin, T. Magnuson, D. Samols, A rapid procedure to identify newborn transgenic mice, DNA 8 (1989) 297-299.
14. C. Abbott, Genomic analysis of transgenic animals by the polymerase chain reaction, in: D. Murphy, D.A. Carter (Eds.), Transgenesis Techniques: Principles and Protocols, Humana Press, Totowa, NJ, 1993, pp. 317-321.
15. J.F. Couse, V.L. Davis, W.C. Tally, K.S. Korach, An improved method of genomic DNA extraction for screening transgenic mice, BioTechniques 17 (1994) 1030-1032.
16. P.W. Laird, A. Zijderveld, K. Linders, M.A. Rudnicki, R. Jaenisch, A. Berns, Simplified mammalian DNA isolation procedure, Nucleic Acids Res. 19 (1991) 4293.
17. T. Hanley, J.P. Merlie, Transgene detection in unpurified mouse tail DNA by polymerase chain reaction, BioTechniques 10 (1991) 56.
18. R. Drews, W.N. Drohan, H. Lubon, Transgene detection in mouse tail digests, BioTechniques 17 (1994) 866-867.
19. C. Henneberger, R. Grantyn, T. Rothe, Rapid genotyping of newborn gene mutant mice, J. Neurosci. Methods 100 (2000) 123-126.
20. C.A. Burkhart, M.D. Norris, M. Haber, A simple method for the isolation of genomic DNA from mouse tail free of real-time PCR inhibitors, J. Biochem. Biophys. Methods 52 (2002) 145-149.
21. N.D. Allen, S.C. Barton, M.A.H. Surani, W. Reil, Production of transgenic mice, in: M. Monk (Ed.), Mammalin Development: A Practical Approach, IRL Press, Oxford, UK, 1987, pp. 217-233.
22. M. Gendron-Maguire, T. Gridley, Identification of transgenic mice, Methods Enzymol. 225 (1993) 794-799.
23. D. Murphy, Isolation of genomic DNA from tail tissue, in: D. Murphy, D.A. Carter (Eds.), Transgenesis Techniques: Principles and Protocols, Humana Press, Totowa, NJ, 1993, pp. 309-312.
24. K.M. Abraham, N.S. Longo, J.A. Hewitt, Detection of transgene integrants and homologous recombinants in mice by polymerase chain reaction, Methods Mol. Biol. 92 (1998) 245-250.
25. B.T. Tinkle, G. Jay, Analysis of transgene integration, in: C.A. Pinkert (Ed.), Transgenic Animal Technology: A Laboratory Manual, Elsevier Science, San Diego, 2002, pp. 459-473.
26. J.W. Voncken, Genetic modification of the mouse: general technology-Pronuclear and blastocyst injection, Methods Mol. Biol. 209 (2003) 9-34.
27. A. Nagy, M. Gertsenstein, K. Vintersten, R. Behringer, Manipulating the Mouse Embryo, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2003.
28. L.F.M. van Zutphen, M. Balls, Animal Alternatives, Welfare, and Ethics, Elsevier Science, Amsterdam, 1997.
29. R.D. Palmiter, R.L. Brinster, R.E. Hammer, M.E. Trumbauer, M.G. Rosenfeld, N.C. Birnberg, R.M. Evans, Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes, Nature 300 (1982) 611-615.
30. C.A. Pinkert, A rapid procedure to evaluate foreign DNA transfer into mammals, BioTechniques 9 (1990) 38-39.
31. M.E. Stevens, J.J. Meneses, R.A. Pedersen, Expression of a mouse metatiothionem-Escherichia coli beta-galactosidase fusion gene (MT-beta gal) in early mouse embryos, Exp. Cell Res. 183 (1989) 319-325.
32. L.L. Nielsen, R.A. Pedersen, Drosophila alcohol dehydrogenase: a novel reporter gene for use in mammalian embryos, J. Exp. Zool. 257 (1991) 128-133.
33. M. Kato, K. Yamanouchi, M. Ikawa, M. Okabe, K. Naito, H. Tojo, Efficient selection of transgenic mouse embryos using EGFP as a marker gene, Mol. Reprod. Dev. 54 (1999) 43-48.
34. F.C. Kafatos, C.W. Jones, A. Efstratiadis, Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure, Nucleic Acids Res. 7 (1979) 1541-1552.
35. D. Murphy, Slot blotting of genomic tail DNA, in: D. Murphy, D.A. Carter (Eds.), Transgenesis Techniques: Principles and Protocols, Humana Press, Totowa, NJ, 1993, pp. 313-315.
36. E.M. Southern, Detection of specific sequences among DNA fragments separated by gel electrophoresis, J. Mol. Biol. 98 (1975) 503-517.
37. D.R. Smith, D. Murphy, Genomic analysis of transgenic animals, in: D. Murphy, D.A. Carter (Eds.), Transgenesis Techniques: Principles and Protocols, Humana Press, Totowa, NJ, 1993, pp. 323-327.
38. A. Joyner, Gene Targeting, Oxford University Press, Oxford, UK, 2000.
39. T. Hamada, H. Sasaki, R. Seki, Y. Sakaki, Mechanism of chromosomal integration of transgenes in microinjected mouse eggs: sequence analysis of genome-transgene and transgene-transgene junctions at two loci, Gene 128 (1993) 197-202.
40. K.M. Pawlik, C.W. Sun, N.P. Higgins, T.M. Townes, End joining of genomic DNA and transgene DNA in fertilized mouse eggs, Gene 165 (1995) 173-181.
41. M. McFarlane, J.B. Wilson, A model for the mechanism of precise integration of a microinjected transgene, Transgenic Res. 5 (1996) 171-177.
42. T.M. Wilkie, R.L. Brinster, R.D. Palmiter, Germline and somatic mosaicism in transgenic mice, Dev. Biol. 118 (1986) 9-18.
43. K. Reue, S. Rehnmark, Screening transgenic mice by PCR: detection of tandem repeats, BioTechniques 17 (1994) 252-253.
44. M.H. Irwin, W.K. Pogozelski, C.A. Pinkert, PCR optimization for detection of transgene integration, in: C.A. Pinkert (Ed.), Transgenic Animal Technology: A Laboratory Manual, Elsevier Science, San Diego, 2002, p. 459.
45. M. Sato, K. Kasai, N. Tada, A sensitive method of testing for transgenic mice using polymerase chain reaction-Southern hybridization, Genet. Anal. 12 (1995) 109-111.
46. B. Aigner, G. Brem, Detection of homozygous individuals in gene transfer experiments by semiquantitative PCR, BioTechniques 18 (1995) 754-758.
47. G. Chatelain, G. Brun, D. Michel, Screening of homozygous transgenic mice by comparative PCR, BioTechniques 18 (1995) 958-962.
48. M.D. Ringel, W.F. Schwindinger, M. Saji, M.A. Zeiger, M.A. Levine, Determination of transgene copy number and expression level using denaturing gradient gel electrophoresis, BioTechniques 24 (1998) 126-131.
49. L. Tesson, J.M. Heslan, S. Menoret, I. Anegon, Rapid and accurate determination of zygosity in transgenic animals by real-time quantitative PCR, Transgenic Res. 11 (2002) 43-48.
50. H. Shitara, A. Sato, J. Hayashi, N. Mizushima, H. Yonekawa, C. Taya, Simple method of zygosity identification in transgenic mice by real-time quantitative PCR, Transgenic Res. 13 (2004) 191-194.
51. M. Ballester, A. Castello, E. Ibanez, A. Sanchez, J.M. Folch, Real-time quantitative PCR-based system for determining transgene copy number in transgenic animals, BioTechniques 37 (2004) 610-613.
52. G. Winberg, A rapid method for preparing DNA from blood, suited for PCR screening of transgenes in mice, PCR Methods Appl. 1 (1991) 72-74.
53. J.E. Dinchuk, K. Kelley, A.L. Boyle, Fluorescence in situ hybridization of interphase nuclei isolated from whole blood of transgenic mice, BioTechniques 17 (1994) 954-961.
54. R.R. Swiger, J.D. Tucker, J.A. Heddle, Detection of transgenic animals without cell culture using fluorescence in situ hybridization, BioTechniques 18 (1995) 952-958.
55. C.A. Gregory, Y. Myal, R.P. Shiu, Rapid genotyping of transgenic mice using dried blood spots from Guthrie cards for PCR analysis, BioTechniques 18 (1995) 758-760.
56. D.B. Campbell, E.J. Hess, Rapid genotyping of mutant mice using dried blood spots for polymerase chain reaction (PCR) analysis, Brain Res. Brain Res. Protoc. 1 (1997) 117-123.
57. M. Ohhara, Y. Kurosu, M. Esumi, Direct PCR of whole blood and hair shafts by microwave treatment, BioTechniques 17 (1994) 726-728.
58. M. Malumbres, R. Mangues, N. Ferrer, S. Lu, A. Pellicer, Isolation of high molecular weight DNA for reliable genotyping of transgenic mice, BioTechniques 22 (1997) 1114-1119.
59. E.M. Schmitteckert, C.M. Prokop, H.J. Hedrich, DNA detection in hair of transgenic mice: a simple technique minimizing the distress on the animals, Lab. Anim. 33 (1999) 385-389.
60. K. Zimmermann, H.P. Schwarz, P.L. Turecek, Deoxyribonucleic acid preparation in polymerase chain reaction genotyping of transgenic mice, Comp. Med. 50 (2000) 314-316.
61. M. Meldgaard, P.J. Bollen, B. Finsen, Non-invasive method for sampling and extraction of mouse DNA for PCR, Lab. Anim. 38 (2004) 413-417.
62. H. Lahm, A. Hoeflich, N. Rieger, R. Wanke, E. Wolf, Identification of transgenic mice by direct PCR analysis of lysates of epithelial cells obtained from the inner surface of the rectum, Transgenic Res. 7 (1998) 131-134.
63. R.L. Broome, L. Feng, Q. Zhou, A. Smith, N. Hahn, S.M. Matsui, M.B. Omary, Non-invasive transgenic mouse genotyping using stool analysis, FEBS Lett. 462 (1999) 159-160.
64. S.Z. Chen, G.A. Evans, A simple screening method for transgenic mice using the polymerase chain reaction, BioTechniques 8 (1990) 32-33.
65. D.G. Skalnik, S. Orkin, A rapid method for characterizing transgenic mice, BioTechniques 8 (1990) 34.
66. J. Attal, M. Cajero-Juarez, L.M. Houdebine, A simple method of DNA extraction from whole tissues and blood using glass powder for detection of transgenic animals by PCR, Transgenic Res. 4 (1995) 149-150.
67. J.R. Hofstetter, A. Zhang, A.R. Mayeda, T. Guscar, J.I. Nurnberger Jr., D.K. Lahiri, Genomic DNA from mice: a comparison of recovery methods and tissue sources, Biochem. Mol. Med. 62 (1997) 197-202.
68. N.L. Nadon, K. Draeger, Genomic DNA analysis from mouse toe lysates, Transgenic Res. 5 (1996) 209-211.
69. M.H. Irwin, R.J. Moffatt, C.A. Pinkert, Identification of transgenic mice by PCR analysis of saliva, Nat. Biotechnol. 14 (1996) 1146-1148.