DNA detection in hair of transgenic mice - A simple technique minimizing the distress on the animals

Laboratory Animals

Volumn 33, Issue 4, 1999, Pages 385-389

  Schmitteckert, Eva Maria ^a   Prokop, Christa Maria ^b   Hedrich, Hans J ^b  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

DNA detection; Hair bulbs; Polymerase chain reaction; Transgenic mice

Indexed keywords

DNA;

ANIMAL TISSUE; ANIMAL WELFARE; ARTICLE; CONTROLLED STUDY; DNA DETERMINATION; HAIR ROOT; LYSIS; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; TECHNIQUE; TRANSGENE;

ANIMALIA; MUS MUSCULUS;

EID: 0032854389     PISSN: 00236772     EISSN: None     Source Type: Journal    
DOI: 10.1258/002367799780487922     Document Type: Article

References (11)

1. Drews R, Drohan WN, Lubon H (1994) Transgene detection in mouse tail digests. Biotechniques 17, 866-7
2. Hanley T, Merlie JP (1991) Transgene detection in unpurified mouse tail DNA by polymerase chain reaction. Biotechniques 10, 56
3. Higuchi R, von Beroldingen CH, Sensabaugh GF, Erlich HA (1988) DNA typing from single hairs. Nature 332, 543-6
4. Hogan B, Beddington R, Costantini F, Lacy E (1994) Manipulating the Mouse Embryo. A Laboratory Manual, 2nd edn. New York: Cold Spring Harbor Laboratory Press, p. 296
5. Laird PW, Zijderveld A, Linders K, Rudnicki MA, Jaenisch R, Berns A (1991) Simplified mammalian DNA isolation procedure. Nucleic Acids Research 19, 4293
6. Pfitzinger H, Ludes B, Mangin P (1993) Sex determination of forensic samples: co-amplification and simultaneous detection of a Y-specific and an X-specific DNA sequence. Journal of Legal Medicine 105, 213-16
7. Reifenberg K, Löhler J, Pudollek H-P, et al. (1997) Long-term expression of the hepatitis B virus core-e and X-proteins does not cause pathologic changes in transgenic mice. Journal of Hepatology 26, 119-30
8. Saiki RK, Gelfand DH, Stoffel S, et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostabile DNA polymerase. Science 239, 487-91
9. Schiwy P, Harmony T (1987). Deutsche Tierschutzgesetze mit Kommentar (Stand 1. Okt. 1996). Starnberg: Verlag R. S. Schulz GmbH
10. Sligh JE, Ballantyne CM, Rich SS, et al. (1993) Inflammatory and immune responses are impaired in mice deficient in intercellular adhesion molecule 1. Proceedings of National Academy of Science USA 90, 8529-33
11. Tierschutzgesetz (TSchG) (1993) Bundesgesetzblatt I, 254