Simple method of zygosity identification in transgenic mice by real-time quantitative PCR

Transgenic Research

Volumn 13, Issue 2, 2004, Pages 191-194

  Shitara, Hiroshi ^a,b   Sato, Akitsugu ^a,c   Hayashi, Jun Ichi ^c   Mizushima, Noboru ^d,e   Yonekawa, Hiromichi ^a,b,f   Taya, Choji ^a  

^a TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE   (Japan)

^b NEW ENERGY AND INDUSTRIAL TECHNOLOGY DEVELOPMENT ORGANIZATION   (Japan)

^c UNIVERSITY OF TSUKUBA   (Japan)

^d JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^e NATIONAL INSTITUTE FOR BASIC BIOLOGY   (Japan)

^f Bio oriented Technol Res Adv I   (Japan)

Author keywords

CYBR Green PCR; Real time quantitative PCR; Transgenic mice; Zygosity

Indexed keywords

FLUORESCENT DYE;

ANIMAL; ARTICLE; GENETICS; HETEROZYGOTE; HETEROZYGOTE DETECTION; HOMOZYGOTE; METHODOLOGY; MOLECULAR GENETICS; MOUSE; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; TRANSGENIC MOUSE;

ANIMALS; BASE SEQUENCE; FLUORESCENT DYES; HETEROZYGOTE; HETEROZYGOTE DETECTION; HOMOZYGOTE; MICE; MICE, TRANSGENIC; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION;

ANIMALIA; MUS MUSCULUS; RODENTIA;

EID: 3242879187     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:TRAG.0000026084.32492.eb     Document Type: Article

References (5)

1. ABI PRISM (1997) ABI PRISM 7700 User Bulletin. PE Applied Biosystems, Foster City, No. 2, pp. 11-15.
2. Gordon JW (1993) Production of transgenic mice. In: Wassarman PM and DePamphilis ML (ed) Methods in Enzymology. Vol. 225 (pp. 747-781) Academic Press, San Diego.
3. Nagy A, Gertsenstein M, Vintersten K and Behringer R (2003) Manipulating the Mouse Embryo. 3rd edn, Cold Spring Habor Laboratory Press, Cold Spring Habor, New York.
4. Shitara H, Kaneda H, Sato A, Iwasaki K, Hayashi JI, Taya C, et al. (2001) Non-invasive visualization of sperm mitochondria behavior in transgenic mice with introduced green fluorescent protein (GFP). FEBS Lett. 500: 7-11.
5. Tesson L, Heslan JM, Ménoret S and Anegon I (2002) Rapid and accurate determination of zygosity in transgenic animals by eral-time quantitative PCR. Transgenic Res 11: 43-18.