Characterization of bacterial drug antiporters homologous to mammalian neurotransmitter transporters

Journal of Bacteriology

Volumn 187, Issue 21, 2005, Pages 7518-7525

  Vardy, Eyal ^a   Steiner Mordoch, Sonia ^a   Schuldiner, Shimon ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

AMIKACIN; AMOXICILLIN PLUS CLAVULANIC ACID; AMPICILLIN; BACTERIAL PROTEIN; CARRIER PROTEIN; CEFALOTIN; CEFTAZIDIME; CEFUROXIME; CHLORAMPHENICOL; COLISTIN; COTRIMOXAZOLE; ERYTHROMYCIN; FUSIDIC ACID; GENTAMICIN; GLYCOPROTEIN P; IMIPENEM; MEROPENEM; NEUROTRANSMITTER; NEUROTRANSMITTER TRANSPORTER; OFLOXACIN; OXACILLIN; PIPERACILLIN PLUS TAZOBACTAM; QUINOLINE DERIVED ANTIINFECTIVE AGENT; STREPTOMYCIN; SULFANILAMIDE DERIVATIVE; TETRACYCLINE; TIMENTIN; UNCLASSIFIED DRUG; UNINDEXED DRUG; VANCOMYCIN; VESICULAR MONOAMINE TRANSPORTER;

AMINO ACID SEQUENCE; ANTIBIOTIC RESISTANCE; ARCHAEBACTERIUM; ARTICLE; BACTERIAL GENOME; CORYNEBACTERIUM GLUTAMICUM; ESCHERICHIA COLI; HALOBACTERIUM SALINARUM; MAMMAL; MOLECULAR CLONING; MYCOBACTERIUM SMEGMATIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN FAMILY; PROTEIN FUNCTION; PROTEIN TRANSPORT;

AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; ANTIPORTERS; ARCHAEA; BACTERIA; BACTERIAL PROTEINS; BIOLOGICAL TRANSPORT; CHLORAMPHENICOL; CLONING, MOLECULAR; CORYNEBACTERIUM GLUTAMICUM; DRUG RESISTANCE, MULTIPLE, BACTERIAL; ESCHERICHIA COLI; FLUOROQUINOLONES; GENES, BACTERIAL; HALOBACTERIUM SALINARUM; MOLECULAR SEQUENCE DATA; MYCOBACTERIUM SMEGMATIS; PHYLOGENY; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

ARCHAEA; BACTERIA (MICROORGANISMS); CORYNEBACTERIUM GLUTAMICUM; ESCHERICHIA COLI; HALOBACTERIUM SALINARUM; MAMMALIA; MYCOBACTERIUM SMEGMATIS;

EID: 27144520233     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.187.21.7518-7525.2005     Document Type: Article

References (41)

1. Abramson, J., I. Smirnova, V. Kasho, G. Verner, H. R. Kaback, and S. Iwata. 2003. Structure and mechanism of the lactose permease of Escherichia coli. Science 301:610-615.
2. Altschul, S. F., T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
3. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
4. Doyle, D. A., J. Morais Cabral, R. A. Pfuetzner, A. Kuo, J. M. Gulbis, S. L. Cohen, B. T. Chait, and R. MacKinnon. 1998. The structure of the potassium channel: molecular basis of K+ conduction and selectivity. Science 280: 69-77.
5. Dutzler, R., E. B. Campbell, M. Cadene, B. T. Chait, and R. MacKinnon. 2002. X-ray structure of a ClC chloride channel at 3.0 A reveals the molecular basis of anion selectivity. Nature 415:287-294.
6. Edgar, R., and E. Bibi. 1997. MdfA, an Escherichia coli multidrug resistance protein with an extraordinarily broad spectrum of drug recognition. J. Bacteriol. 179:2274-2280.
7. Goldberg, E. B., T. Arbel, J. Chen, R. Karpel, G. A. Mackie, S. Schuldiner, and E. Padan. 1987. Characterization of a Na+/H+ antiporter gene of Escherichia coli. Proc. Natl. Acad. Sci. USA 84:2615-2619.
8. Gottesman, M. M., I. Pastan, and S. V. Ambudkar. 1996. P-glycoprotein and multidrug resistance. Curr. Opin. Genet. Dev. 6:610-617.
9. Gutman, N., S. Steiner-Mordoch, and S. Schuldiner. 2003. An amino acid cluster around the essential Glu-14 is part of the substrate-and proton-binding domain of EmrE, a multidrug transporter from Escherichia coli. J. Biol. Chem. 278:16082-16087.
10. Huang, Y., M. J. Lemieux, J. Song, M. Auer, and D. N. Wang. 2003. Structure and mechanism of the glycerol-3-phosphate transporter from Escherichia coli. Science 301:616-620.
11. Iwaki, S., N. Tamura, T. Kimura-Someya, S. Nada, and A. Yamaguchi. 2000. Cysteine-scanning mutagenesis of transmembrane segments 4 and 5 of the Tn10-encoded metal-tetracycline/H+ antiporter reveals a permeability barrier in the middle of a transmembrane water-filled channel. J. Biol. Chem. 275:22704-22712.
12. Jager, W., J. Kalinowski, and A. Puhler. 1997. A Corynebacterium glutamicum gene conferring multidrug resistance in the heterologous host Escherichia coli. J. Bacteriol. 179:2449-2451.
13. Jiang, Y., A. Lee, J. Chen, M. Cadene, B. T. Chait, and R. MacKinnon. 2002. Crystal structure and mechanism of a calcium-gated potassium channel. Nature 417:515-522.
14. + channel. Nature 423:33-41.
15. Kaback, H. R. 1971. Bacterial membranes. Methods Enzymol. 22:99-120.
16. Lewinson, O., J. Adler, G. J. Poelarends, P. Mazurkiewicz, A. J. Driessen, and E. Bibi. 2003. The Escherichia coli multidrug transporter MdfA catalyzes both electrogenic and electroneutral transport reactions. Proc. Natl. Acad. Sci. USA 100:1667-1672.
17. Li, X. Z., L. Zhang, and H. Nikaido. 2004. Efflux pump-mediated intrinsic drug resistance in Mycobacterium smegmatis. Antimicrob. Agents Chemother. 48:2415-2423.
18. Liu, Y., D. Peter, A. Roghani, S. Schuldiner, G. G. Prive, D. Eisenberg, N. Brecha, and R. H. Edwards. 1992. A cDNA that suppresses MPP+ toxicity encodes a vesicular amine transporter. Cell 70:539-551.
19. Marger, M. D., and M. H. Saier, Jr. 1993. A major superfamily of transmembrane facilitators that catalyse uniport, symport and antiport. Trends Biochem. Sci. 18:13-20.
20. Merickel, A., H. R. Kaback, and R. H. Edwards. 1997. Charged residues in transmembrane domains II and XI of a vesicular monoamine transporter form a charge pair that promotes high affinity substrate recognition. J. Biol. Chem. 272:5403-5408.
21. Miroux, B., and J. E. Walker. 1996. Overproduction of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J. Mol. Biol. 260:289-298.
22. Mortimer, P. G., and L. J. Piddock. 1991. A comparison of methods used for measuring the accumulation of quinolones by Enterobacteriaceae, Pseudomonas aeruginosa and Staphylococcus aureus. J. Antimicrob. Chemother. 28:639-653.
23. Muth, T. R., and S. Schuldiner. 2000. A membrane-embedded glutamate is required for ligand binding to the multidrug transporter EmrE. EMBO J. 19:234-240.
24. Ng, E. Y., M. Trucksis, and D. C. Hooper. 1994. Quinolone resistance mediated by norA: physiologic characterization and relationship to flqB, a quinolone resistance locus on the Staphylococcus aureus chromosome. Antimicrob. Agents Chemother. 38:1345-1355.
25. Nicholas, K. B., H. B. Nicholas, Jr, and D. W. Deerfield. 1997. GeneDoc: analysis and visualization of genetic variation. EMBnet News Online 4. http://acer.gen.tcd.ie/embnet.news/vol4_2.
26. Nikaido, H. 1994. Prevention of drug access to bacterial targets: permeability barriers and active efflux. Science 264:382-388.
27. Paulsen, I. T., M. H. Brown, and R. A. Skurray. 1996. Proton-dependent multidrug efflux systems. Microbiol. Rev. 60:575-608.
28. Paulsen, I. T., and R. A. Skurray. 1993. Topology, structure and evolution of two families of proteins involved in antibiotic and antiseptic resistance in eukaryotes and prokaryotes-an analysis. Gene 124:1-11.
29. Perriere, G., and M. Gouy. 1996. WWW-query: an on-line retrieval system for biological sequence banks. Biochimie 78:364-369.
30. Putman, M., H. W. van Veen, and W. N. Konings. 2000. Molecular properties of bacterial multidrug transporters. Microbiol. Mol. Biol. Rev. 64:672-693.
31. Rotem, D., and S. Schuldiner. 2004. EmrE, a multidrug transporter from Escherichia coli, transports monovalent and divalent substrates with the same stoichiometry. J. Biol. Chem. 279:48787-48793.
32. Saier, M. H., Jr., and I. T. Paulsen. 2001. Phylogeny of multidrug transporters. Semin. Cell Dev. Biol. 12:205-213.
33. Sambrook, J., R. D. 2001. Molecular cloning: a laboratory manual, 3rd ed., vol. 1-3. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
34. Schuldiner, S., A. Shirvan, and M. Linial. 1995. Vesicular neurotransmitter transporters: from bacteria to humans. Physiol. Rev. 75:369-392.
35. Tabor, S., and C. C. Richardson. 1985. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. USA 82:1074-1078.
36. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
37. Vardy, E., I. T. Arkin, K. E. Gottschalk, H. R. Kaback, and S. Schuldiner. 2004. Structural conservation in the major facilitator superfamily as revealed by comparative modeling. Protein Sci. 13:1832-1840.
38. Viklund, H., and A. Elofsson. 2004. Best alpha-helical transmembrane protein topology predictions are achieved using hidden Markov models and evolutionary information. Protein Sci. 13:1908-1917.
39. World Health Organization. 2005. Global tuberculosis control- surveillance, planning, financing (W.H.O./HTM/TB/2005.349). World Health Organization, Geneva, Switzerland.
40. Yanisch-Perron, C., J. Viera, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119.
41. Yelin, R., and S. Schuldiner. 1995. The pharmacological profile of the vesicular monoamine transporter resembles that of multidrug transporters. FEBS Lett. 377:201-207.