Preprotein translocase of the outer mitochondrial membrane: Reconstituted Tom40 forms a characteristic TOM pore

Journal of Molecular Biology

Volumn 353, Issue 5, 2005, Pages 1011-1020

  Becker, Lars ^a   Bannwarth, Michael ^b   Meisinger, Chris ^b   Hill, Kerstin ^a   Model, Kirstin ^b,d   Krimmer, Thomas ^b   Casadio, Rita ^c   Truscott, Kaye N ^b,e   Schulz, Georg E ^b   Pfanner, Nikolaus ^b   Wagner, Richard ^a  

^a UNIVERSITY OF OSNABRÜCK   (Germany)

^b UNIVERSITY OF FREIBURG   (Germany)

^c UNIVERSITY OF BOLOGNA   (Italy)

^d MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^e LA TROBE UNIVERSITY   (Australia)

Author keywords

Mitochondria; Neurospora crassa; Protein sorting; Saccharomyces cerevisiae; TOM complex

Indexed keywords

CARRIER PROTEIN; PROTEIN PRECURSOR;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CIRCULAR DICHROISM; CONTROLLED STUDY; ELECTROPHYSIOLOGY; ENZYME RECONSTITUTION; MEMBRANE VESICLE; MITOCHONDRIAL MEMBRANE; NEUROSPORA CRASSA; NONHUMAN; OUTER MEMBRANE; PREDICTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STRUCTURE; PROTEIN TARGETING; RENATURATION; SACCHAROMYCES CEREVISIAE;

NEUROSPORA CRASSA; SACCHAROMYCES CEREVISIAE;

EID: 27144464893     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2005.09.019     Document Type: Article

References (36)

1. W. Neupert Protein import into mitochondria Annu. Rev. Biochem. 66 1997 863 917
2. K. Gabriel, S.K. Buchanan, and T. Lithgow The alpha and the beta: protein translocation across mitochondrial and plastid outer membranes Trends Biochem. Sci. 26 2001 36 40
3. N. Pfanner, and N. Wiedemann Mitochondrial protein import: two membranes, three translocases Curr. Opin. Cell Biol. 14 2002 400 411
4. K. Mihara Cell biology: moving inside membranes Nature 424 2003 505 506
5. T. Endo, H. Yamamoto, and M. Esaki Functional cooperation and separation of translocators in protein import into mitochondria, the double-membrane bounded organelles J. Cell Sci. 116 2003 3259 3267
6. K. Gabriel, B. Egan, and T. Lithgow Tom40, the import channel of the mitochondrial outer membrane, plays an active role in sorting imported proteins EMBO J. 15 2003 2380 2386
7. P. Rehling, N. Pfanner, and C. Meisinger Insertion of hydrophobic membrane proteins into the inner mitochondrial membrane - a guided tour J. Mol. Biol. 326 2003 639 657
8. N. Pfanner, N. Wiedemann, C. Meisinger, and T. Lithgow Assembling the mitochondrial outer membrane Nature Struct. Mol. Biol. 11 2004 1044 1048
9. K.P. Baker, A. Schaniel, D. Vestweber, and G. Schatz A yeast mitochondrial outer membrane protein essential for protein import and cell viability Nature 348 1990 605 609
10. S. van Wilpe, M.T. Ryan, K. Hill, A.C. Maarse, C. Meisinger, and J. Brix Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase Nature 401 1999 485 489
11. K. Hill, K. Model, M.T. Ryan, K. Dietmeier, F. Martin, R. Wagner, and N. Pfanner Tom40 forms the hydrophilic channel of the mitochondrial import pore for preproteins Nature 395 1998 516 521
12. D.M. Gordon, J. Wang, B. Amutha, and D. Pain Self-association and precursor protein binding of Saccharomyces cerevisiae Tom40p, the core component of the protein translocation channel of the mitochondrial outer membrane Biochem. J. 356 2001 207 215
13. C.A. Mannella, A.F. Neuwald, and C.E. Lawrence Detection of likely transmembrane β-strand regions in sequences of mitochondrial pore proteins using the Gibbs sampler J. Bioenerg. Biomembr. 28 1996 163 169
14. K.P. Künkele, S. Heins, M. Dembowski, F.E. Nargang, R. Benz, and M. Thieffry The preprotein translocation channel of the outer membrane of mitochondria Cell 93 1998 1009 1019
15. K.P. Künkele, P. Juin, C. Pompa, F.E. Nargang, J.P. Henry, and W. Neupert The isolated complex of the translocase of the outer membrane of mitochondria. Characterization of the cation-selective and voltage-gated preprotein-conducting pore J. Biol. Chem. 273 1998 31032 31039
16. U. Ahting, C. Thun, R. Hegerl, D. Typke, F.E. Nargang, W. Neupert, and S. Nussberger The TOM core complex: the general protein import pore of the outer membrane of mitochondria J. Cell Biol. 147 1999 959 968
17. C. Meisinger, M.T. Ryan, K. Hill, K. Model, J.H. Lim, and A. Sickmann Protein import channel of the outer mitochondrial membrane: a highly stable Tom40-Tom22 core structure differentially interacts with preproteins, small Tom proteins, and import receptors Mol. Cell. Biol. 21 2001 2337 2348
18. K. Model, T. Prinz, T. Ruiz, M. Radermacher, T. Krimmer, and W. Kühlbrandt Protein translocase of the outer mitochondrial membrane: role of import receptors in the structural organization of the TOM complex J. Mol. Biol. 316 2002 657 666
19. U. Ahting, M. Thieffry, H. Engelhardt, R. Hegerl, W. Neupert, and S. Nussberger Tom40, the pore-forming component of the protein-conducting TOM channel in the outer membrane of mitochondria J. Cell Biol. 153 2001 1151 1160
20. M. Bannwarth, and G.E. Schulz The expression of outer membrane proteins for crystallization Biochim. Biophys. Acta 1610 2003 37 45
21. G. Böhm, R. Muhr, and R. Jaenicke Quantitative analysis of protein far UV circular dichroism spectra by neural networks Protein Eng. 5 1992 191 195
22. N. Sreerama, and R.W. Woody Estimation of protein secondary structure from circular dichroism spectra: comparison of CONTIN, SELCON, and CDSSTR methods with an expanded reference set Anal. Biochem. 287 2000 252 260
23. N. Sreerama, and R.W. Woody Structural composition of betaI- and betaII-proteins Protein Sci. 12 2003 384 388
24. N. Sreerama, and R.W. Woody On the analysis of membrane protein circular dichroism spectra Protein Sci. 13 2004 100 112
25. I. Jacoboni, P.L. Martelli, P. Fariselli, V. De Pinto, and R. Casadio Prediction of the transmembrane regions of β-barrel membrane proteins with a neural network-based predictor Protein Sci. 10 2001 779 787
26. P.L. Martelli, P. Fariselli, A. Krogh, and R. Casadio A sequence-profile-based HMM for predicting and discriminating β barrel membrane proteins Bioinformatics 18 2002 S46 S53
27. K.N. Truscott, P. Kovermann, A. Geissler, A. Merlin, M. Meijer, and A.J.M. Driessen A presequence- and voltage-sensitive channel of the mitochondrial preprotein translocase formed by Tim23 Nature Struct. Biol. 8 2001 1074 1082
28. P. Kovermann, K.N. Truscott, B. Guiard, P. Rehling, N.B. Sepuri, and H. Müller Tim22, the essential core of the mitochondrial protein insertion complex, forms a voltage-activated and signal-gated channel Mol. Cell 9 2002 363 373
29. S. Hinnah, K. Hill, R. Wagner, T. Schlicher, and J. Soll Reconstitution of a chloroplast protein import channel EMBO J. 16 1997 7351 7360
30. S.C. Hinnah, R. Wagner, N. Sveshnikova, R. Harrer, and J. Soll The chloroplast protein import channel Toc75: pore properties and interaction with transit peptides Biophys. J. 83 2002 899 911
31. A. Wirth, M. Jung, C. Bies, M. Frien, J. Tyedmers, R. Zimmermann, and R. Wagner The Sec61p complex is a dynamic precursor activated channel Mol Cell. 12 2003 261 268
32. T. Stan, U. Ahting, M. Dembowski, K.P. Künkele, S. Nussberger, W. Neupert, and D. Rapaport Recognition of preproteins by the isolated TOM complex of mitochondria EMBO J. 19 2000 4895 4902
33. H. Suzuki, T. Kadowaki, M. Maeda, H. Sasaki, J. Nabekura, M. Sakaguchi, and K. Mihara Membrane-embedded C-terminal segment of rat mitochondrial TOM40 constitutes protein-conducting pore with enriched β-structure J. Biol. Chem. 279 2004 50619 50629
34. J. Brix, K. Dietmeier, and N. Pfanner Differential recognition of preproteins by the purified cytosolic domains of the mitochondrial import receptors Tom20, Tom22 and Tom70 J. Biol. Chem. 272 1997 20730 20735
35. Y. Abe, T. Shodai, T. Muto, K. Mihara, H. Torii, and S. Nishikawa Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20 Cell 100 2000 551 560
36. M. Bannwarth, and G.E. Schulz Asymmetric conductivity of engineered porins Protein Eng. 15 2002 799 804