Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase

Nature

Volumn 401, Issue 6752, 1999, Pages 485-489

  Van Wilpe, Sandra ^a   Ryan, Michael T ^b   Hill, Kerstin ^c   Maarse, Ammy C ^a   Meisinger, Chris ^b   Brix, Jan ^b   Dekker, Peter J T ^b   Moczko, Martin ^b   Wagner, Richard ^c   Meijer, Michiel ^a   Gulard, Bernard ^d   Hönlinger, Angelika ^b   Pfanner, Nikolaus ^b  

^a Institute for Molecular Cell Biology   (Netherlands)

^b UNIVERSITY OF FREIBURG   (Germany)

^c UNIVERSITY OF OSNABRÜCK   (Germany)

^d CENTRE DE GÉNÉTIQUE MOLÉCULAIRE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; MITOCHONDRIAL DNA; OUTER MEMBRANE PROTEIN; PROTEIN PRECURSOR; RECEPTOR PROTEIN; SULFUR 35;

ARTICLE; CROSS LINKING; ENZYME ACTIVITY; ISOTOPE LABELING; MITOCHONDRIAL MEMBRANE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN TRANSPORT; SACCHAROMYCES CEREVISIAE; YEAST;

BIOLOGICAL TRANSPORT; CARRIER PROTEINS; ELECTROPHYSIOLOGY; FUNGAL PROTEINS; GENE DELETION; INTRACELLULAR MEMBRANES; ION CHANNELS; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MITOCHONDRIA; MITOCHONDRIAL ADP, ATP TRANSLOCASES; POLYMERASE CHAIN REACTION; PROTEIN BINDING; PROTEIN PRECURSORS; RECEPTORS, CELL SURFACE; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SPORES, FUNGAL; TETRAHYDROFOLATE DEHYDROGENASE;

SACCHAROMYCES CEREVISIAE;

EID: 0033619269     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/46802     Document Type: Article

References (26)

1. Schatz, G. & Dobberstein, B. Common principles of protein translocation across membrane. Science 271, 1519-1526 (1996).
2. Neupert, W. Protein import into mitochondria. Annu. Rev. Biochem. 66, 863-917 (1997).
3. Pfanner, N., Craig, E. A. & Hönlinger, A. Mitochondrial preprotein translocase. Annu. Rev. Cell Dev. Biol. 13, 25-51 (1997).
4. Ryan, K. R. & Jensen, R. E. Protein translocation across mitochondrial membranes: what a long, strange trip it is. Cell 83, 517-519 (1995).
5. Schatz, G. Protein transport: the doors to organelles. Nature 395, 439-440 (1998).
6. Hönlinger, A. et al. the mitochondrial receptor complex: Mom22 is essential for cell viability and directly interacts with preproteins. Mol. Cell. Biol. 15, 3382-3389 (1995).
7. Bolliger, L., Junne, T., Schatz, G. & Lithgow, T. Acidic receptor domains on both sides of the outer membrane mediate translocation of precursor proteins into yeast mitochondria. EMBO J. 14, 6318-6326 (1995).
8. Mayer, A., Nargang, F. E., Neupert, W. & Lill, R. MOM22 is a receptor for mitochondrial targeting sequences and cooperates with MOM19. EMBO J. 14, 4205-4211 (1995).
9. Moczko, M. et al. The intermembrane space domain of mitochondrial Tom22 functions as a trans binding site for preproteins with N-terminal targeting sequences. Mol. Cell Biol. 17, 6574-6584 (1997).
10. Brix, J., Dietmeier, K. & Pfanner, N. Differential recognition of preproteins by the purified cytosolic domains of the mitochondrial import receptors Tom20, Tom22, and Tom70. J. Biol. Chem. 272, 20730-20735 (1997).
11. Vestweber, D., Brunner, J., Baker, A. & Schatz, G. A 42K outer-membrane protein is a component of the yeast mitochondrial protein import site. Nature 341, 205-209 (1989).
12. Hill, K. et al. Tom40 forms the hydrophilic channel of the mitochondrial import pore for preproteins. Nature 395, 516-521 (1998).
13. Dekker, P. J. T. et al. The preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex. Mol. Cell. Biol. 18, 6515-6524 (1998).
14. Baker, K. P., Schaniel, A., Vestweber, D. & Schatz, G. A yeast mitochondrial outer membrane protein essential for protein import and cell viability. Nature 348, 605-609 (1990).
15. Lithgow, T., Junne, T., Suda, K., Gratzer, S. & Schatz, G. The mitochondrial outer membrane protein Mas22p is essential for protein import and viability of yeast. Proc. Natl Acad. Sci. USA 91, 11973-11977 (1994).
16. Guthrie, C. & Fink, G. R. Guide to yeast genetics and molecular biology. Methods Enzymol. 194 (Academic, San Diego, California, 1991).
17. Nargang, F. E. et al. 'Sheltered disruption' of Neurospora crassa MOM22, an essential component of the mitochondrial protein import complex. EMBO J. 14, 1099-1108 (1995).
18. Alconada, A., Gärtner, F., Hönlinger, A., Kübrich, M. & Pfanner, N. Mitochondrial receptor complex from Neurospora crassa and Saccharomyces cerevisiae. Methods Enzymol. 260, 263-286 (1995).
19. Söllner, T., Rassow, J. & Pfanner, N. Analysis of mitochondrial protein import using translocaton intermediates and specific antibodies. Methods Cell Biol. 3, 345-358 (1991).
20. Dietmeier, K. et al. Tom5 functionally links mitochondrial preprotein receptors to the general import pore. Nature 388, 195-200 (1997).
21. Hönlinger, A. et al. Tom7 modulates the dynamics of the mitochondrial outer membrane translocase and plays a pathway-related role in protein import. EMBO J. 15, 2125-2137 (1996).
22. Alconada, A., Kübrich, M., Moczko, M., Hönlinger, A. & Pfanner, N. The mitochondrial receptor complex: the small subunit Mom8b/Isp6 supports association of receptors with the general insertion pore and transfer of preproteins. Mol. Cell. Biol. 15, 6196-6205 (1995).
23. Haucke, V., Horst, M., Schatz, G. & Lithgow, T. The Mas20p and Mas70p subunits of the protein import machinery interact via the tetratricopeptide repeat motif in Mas20p: evidence for a single hetero-oligomeric receptor. EMBO J. 15, 1231-1237 (1996).
24. Künkele, K.-P. et al. The preprotein translocation channel of the outer membrane of mitochondria. Cell 93, 1009-1019 (1998).
25. Kassenbrock, C. K., Cao, W. & Douglas, M. G. Genetic and biochemical characterization of ISP6, a small mitochondrial outer membrane protein associated with the protein translocation complex. EMBO J. 8, 3023-3034 (1993).
26. Hinnah, S., Hill, K., Wagner, R., Schlicher, T. & Soll, J. Reconstitution of a chloroplast protein import channel. EMBO J. 16, 7351-7360 (1997).