Localization of replication forks in wild-type and mukB mutant cells of Escherichia coli

Molecular Genetics and Genomics

Volumn 274, Issue 3, 2005, Pages 264-271

  Adachi, Shun ^a   Kohiyama, Masamichi ^b   Onogi, Toshinari ^c,d   Hiraga, Sota ^a,c  

^a KYOTO UNIVERSITY   (Japan)

^b INSTITUT JACQUES MONOD   (France)

^c KUMAMOTO UNIVERSITY   (Japan)

^d CHIBA UNIVERSITY   (Japan)

Author keywords

Bromodeoxyuridine; Chromosome partition; MukB; Nascent DNA; Replication fork

Indexed keywords

BROXURIDINE; MUTANT PROTEIN;

ANIMAL CELL; ARTICLE; BACTERIAL STRAIN; CELL GROWTH; CELL MUTANT; CELLULAR DISTRIBUTION; CHROMOSOME REPLICATION; CONTROLLED STUDY; DNA SYNTHESIS; ESCHERICHIA COLI; GENE MUTATION; IMMUNOFLUORESCENCE TEST; NONHUMAN; PRIORITY JOURNAL; WILD TYPE;

BROMODEOXYURIDINE; CHROMOSOMAL PROTEINS, NON-HISTONE; DNA; DNA REPLICATION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; IMMUNOHISTOCHEMISTRY;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 26944479228     PISSN: 16174615     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00438-005-0023-6     Document Type: Article

References (42)

1. Adachi S, Hiraga S (2003) Mutants suppressing novobiocin hypersensitivity of a mukB null mutant. J Bacteriol 185:3690-3695
2. Blaauwen TD, Lindqvist A, Löwe J, Nanninga N (2001) Distribution of the Escherichia coli structural maintenance of chromosomes (SMC)-like protein MukB in the cell. Mol Microbiol 42:1179-1188
3. Brendler T, Austin S (1999) Binding of SeqA protein to DNA requires interaction between two or more complexes bound to separate hemimethylated GATC sequences. EMBO J 18:2304-2310
4. Brendler T, Sawitzke J, Sergueev K, Austin S (2000) A case for sliding SeqA tracts at anchored replication forks during Escherichia coli chromosome replication and segregation. EMBO J 19:6249-6258
5. Britton RA, Lin CD, Grossman AD (1998) Characterization of a prokaryotic SMC protein involved in chromosome partitioning. Genes Dev 12:1254-1259
6. Gordon GS, Sitnikov D, Webb CD, teleman A, Straight A, Losick R, Murray AW, Wright A (1997) Chromosome and low copy plasmid segregation in E. coli: visual evidence for distinct mechanisms. Cell 90:1113-1121
7. Graumann PL, Losick R, Strunnikov AV (1998) Subcellular localization of Bacillus subtilis SMC, a protein involved in chromosome condensation and segregation. J Bacteriol 180:5749-5755
8. Hiraga S (2000) Dynamic localization of bacterial and plasmid chromosomes. Annu Rev Genet 34:21-59
9. Hiraga S, Niki H, Ogura T, Ichinose C, Mori H, Ezaki B, Jaffé A (1989) Chromosome partitioning in Escherichia coli: novel mutants producing anucleate cells. J Bacteriol 171:1496-1505
10. Hiraga S, Ichinose C, Niki H, Yamazoe M (1998) Cell cycle-dependent duplication and bidirectional migration of SeqA-associated DNA-protein complexes in E. coli. Mol Cell 1:381-387
11. Hiraga S, Ichinose C, Onogi T, Niki H, Yamazoe M (2000) Bidirectional migration of SeqA-bound hemimethylated DNA clusters and pairing of oriC copies in Escherichia coli. Genes Cells 5:327-341
12. Hirano T (1999) SMC-mediated chromosome mechanisms: a conserved scheme from bacteria to vertebrates? Genes Dev 13:11-19
13. Hirano T, Mitchison TJ, Swedlow JR (1995) The SMC family: from chromosome condensation to dosage compensation. Curr Opin Cell Biol 7:329-336
14. Holmes VF, Cozzarelli NR (2000) Closing the ring: links between SMC proteins and chromosome partitioning, condensation, and supercoiling. Proc Natl Acad Sci USA 97:1322-1324
15. Lemon KP, Grossman AD (1998) Localization of bacterial DNA polymerase: evidence for a factory model of replication. Science 282:1516-1519
16. Lewis PJ, Errington J (1997) Direct evidence for active segregation of oriC regions of the Bacillus subtilis chromosome and colocalization with the Spo0J partitioning protein. Mol Microbiol 25:945-954
17. Lindow JC, Britton RA, Grossman AD (2002a) Structural maintenance of chromosomes protein of Bacillus subtilis affects supercoiling in vivo. J Bacteriol 184:5317-5322
18. Lindow JC, Kuwano M, Moriya S, Grossman AD (2002b) Subcellular localization of the Bacillus subtilis structural maintenance of chromosomes (SMC) protein. Mol Microbiol 46:997-1007
19. Lu M, Campbel JL, Boye E, Kleckner N (1994) SeqA: a negative modulator of replication initiation in E. coli. Cell 77:413-426
20. Mascarenhas J, Soppa J, Strunnikov AV, Graumann PL (2002) Cell cycle-dependent localization of two novel prokaryotic chromosome segregation and condensation proteins in Bacillus subtilis that interact with SMC protein. EMBO J 21:3108-3118
21. Melby TE, Ciampaglio CN, Briscore B, Erickson HP (1998) The symmetrical structure of structure maintenance of chromosomes (SMC) and MukB proteins: long, antiparallel coiled coils, folded at a flexible hinge. J Cell Biol 142:1595-1604
22. Miller J (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
23. Moriya S, Tsujikawa E, Hassan AK, Asai K, Kodama T, Ogasawara N (1998) A Bacillus subtilis gene-encoding protein homologous to eukaryotic SMC motor protein is necessary for chromosome partition. Mol Microbiol 29:179-187
24. Niki H, Hiraga S (1998) Polar localization of the replication origin and terminus in Escherichia coli nucleoids during chromosome partitioning. Genes Dev 12:1036-1045
25. Niki H, Jaffé A, Imamura R, Ogura T, Hiraga S (1991) The new gene mukB codes for a 177 kd protein with coiled-coil domains involved in chromosome partitioning of E. coli. EMBO J 10:183-193
26. Niki H, Imamura R, Kitaoka M, Yamanaka K, Ogura T, Hiraga S (1992) E. coli MukB protein involved in chromosome partition forms a homodimer with a rod-and hinge structure having DNA binding and ATP/GTP binding activities. EMBO J 11:5101-5109
27. Niki H, Yamaichi Y, Hiraga S (2000) Dynamic organization of chromosomal DNA in Escherichia coli. Genes Dev 14:212-223
28. Ohsumi K, Yamazoe M, Hiraga S (2001) Different localization of SeqA-bound nascent DNA clusters and MukF-MukE-MukB complex in Escherichia coli cells. Mol Microbiol 40:835-845
29. Onogi T, Niki H, Yamazoe M, Hiraga S (1999) The assembly and migration of SeqA-GFP fusion in living cells of Escherichia coli. Mol Microbiol 31:1775-1782
30. Onogi T, Yamazoe M, Ichinose C, Niki H, Hiraga S (2000) Null mutation of the dam or seqA gene suppresses temperature-sensitive lethality but not hypersensitivity to novobiocin of muk null mutants. J Bacteriol 182:5898-5901
31. Onogi T, Ohsumi K, Katayama T, Hiraga S (2002) Replication-dependent recruitment of the β-subunit of DNA polymerase III from cytosolic spaces to replication forks in Escherichia coli. J Bacteriol 184:867-870
32. Sawitzke JA, Austin S (2000) Suppression of chromosome segregation defects of Escherichia coli muk mutants by mutations in topoisomerase I. Proc Natl Acad Sci USA 97:1671-1676
33. Skarstad K, Steen BH, Boye E (1985) Escherichia coli DNA distributions measured by flow cytometry and compared with theoretical computer simulations. J Bacteriol 163:661-668
34. Slater S, Wold S, Lu M, Boye E, Skarstad K, Kleckner N (1995) E. coli SeqA protein binds oriC in two different methyl-modulated reactions appropriate to its roles in DNA replication initiation and origin sequestration. Cell 82:927-936
35. Soppa J, Derryn E, Ogasawara N, Moriya S (2002) Discovery of two novel families of proteins that are proposed to interact with prokaryotic SMC proteins, and characterization of the Bacillus subtilis family members ScpA and ScpB. Mol Microbiol 45:59-71
36. Sunako Y, Onogi T, Hiraga S (2001) Sister chromosome cohesion of Escherichia coli. Mol Microbiol 42:1233-1241
37. Weitao T, Nordström K, Dasgupta S (1999) Mutual suppression of mukB and seqA phenotypes might arise from their opposing influences on the Escherichia coli nucleoid structure. Mol Microbiol 34:157-168
38. Weitao T, Dasgupta S, Nordström K (2000a) Role of the mukB gene in chromosome and plasmid partition in Escherichia coli. Mol Microbiol 38:392-400
39. Weitao T, Nordström K, Dasgupta S (2000b) Escherichia coli cell cycle control genes affect chromosome superhelicity. EMBO Rep 1:494-499
40. Yamanaka K, Ogura T, Niki H, Hiraga S (1996) Identification of two new genes, mukE and mukF, involved in chromosome partitioning in Escherichia coli. Mol Gen Genet 250:241-251
41. Yamazoe M, Onogi T, Sunako Y, Niki H, Yamanaka K, Ichimura T, Hiraga S (1999) Complex formation of MukB, MukE and MukF proteins involved in chromosome partitioning in Escherichia coli. EMBO J 18:5873-5884
42. Yamazoe M, Adachi S, Kanaya S, Ohsumi K, Hiraga S (2005) Sequential binding of SeqA protein to nascent DNA segments at replication forks in synchronized cultures of Escherichia coli. Mol Microbiol 55:289-298