Sequential binding of SeqA protein to nascent DNA segments at replication forks in synchronized cultures of Escherichia coli

Molecular Microbiology

Volumn 55, Issue 1, 2005, Pages 289-298

  Yamazoe, Mitsuyoshi ^a   Adachi, Shun ^a   Kanaya, Shigehiko ^b   Ohsumi, Katsufumi ^c   Hiraga, Sota ^a  

^a KYOTO UNIVERSITY   (Japan)

^b NARA INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

^c NATIONAL INSTITUTE FOR BASIC BIOLOGY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; BACTERIAL DNA; BACTERIAL PROTEIN; SEQUESTRATION A PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL CHROMOSOME; BACTERIUM CULTURE; CHROMOSOME REPLICATION; CONTROLLED STUDY; DNA METHYLATION; DNA REPLICATION; DNA SEQUENCE; ESCHERICHIA COLI; IMMUNOFLUORESCENCE MICROSCOPY; IMMUNOPRECIPITATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; SEQUENCE ANALYSIS;

BACTERIAL OUTER MEMBRANE PROTEINS; CHROMATIN IMMUNOPRECIPITATION; DNA REPLICATION; DNA, BACTERIAL; DNA-BINDING PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; KINETICS; MICROSCOPY, FLUORESCENCE; PROTEIN BINDING; STAINING AND LABELING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 12344308528     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2958.2004.04389.x     Document Type: Article

References (41)

1. Adachi, S., and Hiraga, S. (2003) Mutants suppressing novobiocin hypersensitivity of a mukB null mutation. J Bacteriol 185: 3690-3695.
2. Aussel, L., Barre, F.-X., Aroyo, M., Stasiak, A.Z., Sherratt, D. (2002) FtsK is a DNA motor protein that activates chromosome dimer resolution by switching the catalytic state of the XerC and XerD recombinases. Cell 108: 195-205.
3. Bach, T., Krekling, M.A., and Skarstad, K. (2003) Excess SeqA prolongs sequestration of oriC and delays nucleoid segregation and cell division. EMBO J 22: 315-323.
4. Bakker, A., and Smith, D.W. (1989) Methylation of GATC sites is required for precise timing between rounds of DNA replication in Escherichia coli. J Bacteriol 171: 5738-5742.
5. Blattner, F.R., Plunkett, G., III, Bloch, C.A., Perna, N.T., Burland, V., Riley, M., et al. (1997) The complete genome sequence of Escherichia coli K-12. Science 277: 1453-1474.
6. Boye, E., Stokke, T., Kleckner, N., and Skarstad, K. (1996) Coordinating DNA replication initiation with cell growth: differential roles for DnaA and SeqA proteins. Proc Natl Acad Sci USA 93: 12206-12211.
7. Brendler, T., Abeles, A., and Austin, S. (1995) A protein that binds to the P1 origin core and the oriC 13mer region in a methylation-specific fashion is the product of the host seqA gene. EMBO J 14: 4083-4089.
8. Brendler, T., and Austin, S. (1999) Binding of SeqA protein to DNA requires interaction between two or more complexes bound to separate hemimethylated GATC sequences. EMBO J 18: 2304-2310.
9. Brendler, T., Sawitzke, J., Sergueev, K., and Austin, S. (2000) A case for sliding SeqA tracts at anchored replication forks during Escherichia coli chromosome replication and segregation. EMBO J 19: 6249-6258.
10. Campbell, J.L, and Kleckner, N. (1990) E. coli oriC and the dnaA gene promoter are sequestered from dam methyltransferase following the passage of the chromosomal replication fork. Cell 62: 967-979.
11. Carl, P.L. (1970) Escherichia coli mutants with temperature-sensitive synthesis of DNA. Mol Gen Genet 109: 107-122.
12. Case, R.B., Chang, Y.-P., Smith, S.B., Gore, J., Cozzarelli. N.R., and Bustamante, C. (2004) The bacterial condensing MukBEF compacts DNA into repetitive, stable structure. Science 305: 222-227.
13. Espeli, O., Levine, C., Hassing, H,, and Marians, K.J. (2003) Temporal regulation of topoisomerase IV actively in E. coli. Mol Cell 11: 189-201.
14. Fossum, S., Søreide, S., and Skarstad, K. (2003) Lack of SeqA focus formation, specific DNA binding and proper protein multimerization in the Escherichia coli sequestration mutant seqA2. Mol Microbiol 47: 619-632.
15. von Freiesleben, U., Rasmussen, K.V., and Schaechter, M. (1994) SeqA limits DnaA activity in replication from oriC. Escherichia coli. Mol Microbiol 14: 763-772.
16. Henaut, A., Rouxel, T., Gleizes, A., Moszer, I., and Danchin, A. (1996) Uneven distribution of GATC motifs in the Escherichia coli chromosome, its plasmids and its phages. J Mol Biol 257: 574-585.
17. Hiraga, S. (2000) Dynamic localization of bacterial and plasmid chromosomes. Annu Rev Genet 34: 21-59.
18. Hiraga, S., Ichinose, C., Niki, H., and Yamazoe, M. (1998) Cell cycle-dependent duplication and bidirectional migration of SeqA-associated DNA-protein complexes in E. coli. Mol Cell 1: 381-387.
19. Hiraga, S., Ichinose, C., Onogi, T., Niki, H., and Yamazoe, M. (2000) Bidirectional migration of SeqA-bound hemimethylated DNA clusters and pairing of oriC copies in Escherichia coli. Genes Cells 5: 327-341.
20. Kang, S., Han, J.S., Park, J.H., Skarstad, K., and Hwang, D.S. (2003) SeqA protein stimulates the relaxing and decatenating activities of topoisomerase IV. J Biol Chem 278: 48779-48785.
21. Lee, H., Kang, S., Bae, S.H., Choi, B.S., and Hwang, D.S. (2001) SeqA protein aggregation is necessary for SeqA function. J Biol Chem 276: 34600-34606.
22. Lemon, K.P., and Grossman, A.D. (1998) Localization of bacterial DNA polymerase: evidence for a factory model of replication. Science 282: 1516-1519.
23. Lemon, K.P., and Grossman, A.D. (2000) Movement of replicating DNA through a stational replisome. Mol Cell 6: 1321-1330.
24. Lin, D.C.-H., and Grossman, A.D. (1998) Identification and characterization of a bacterial chromosome partitioning site. Cell 92: 675-685.
25. Loskshoun, D., and Morris, D.R. (1983) Positively supercoiled plasmid DNA is produced by treatment of Escherichia coli with DNA gyrase inhibitors. Nucleic Acids Res 11: 2999-3017.
26. Lu, M., Campbel, J.L., Boye, E., and Kleckner, N. (1994) SeqA: a negative modulator of replication initiation in E. coli. Cell 77: 413-426.
27. Niki, H., and Hiraga, S. (1998) Polar localization of the replication origin and terminus in Escherichia coli nucleoids during chromosome partitioning. Genes Dev 12: 1036-1045.
28. Niki, H., Yamaichi, Y., and Hiraga, S. (2000) Dynamic organization of chromosomal DNA in Escherichia coli. Genes Dev 14: 212-223.
29. Ogden, G.B., Pratt, M.J., and Schechter, M. (1988) The replicative origin of the E. coli chromosome binds to cell membranes only when hemimethylated. Cell 54: 127-135.
30. Ohsumi, K., Yamazoe, M., and Hiraga, S. (2001) Different localization of SeqA-bound nascent DNA clusters and MukF-MukE-MukB complex in Escherichia coli cells. Mol Microbiol 40: 835-845.
31. Onogi, T., Niki, H., Yamazoe, M., and Hiraga, S. (1999) The assembly and migration of SeqA-Gfp fusion in living cells of Escherichia coli. Mol Microbiol 31: 1775-1782.
32. Onogi, T., Yamazoe, M., Ichinose, C., Niki, H., and Hiraga, S. (2000) Null mutation of the dam or seqA gene supresses temperature-sensitive lethality but not hypersensitivity to novobiocin of muk null mutants. J Bacteriol 182: 5898-5901.
33. Onogi, T., Ohsumi, K., Katayama, T., and Hiraga, S. (2002) Replication-dependent recruitment of the β-subunit of DNA polymerase III from cytosolic spaces to replication forks in Escherichia coli. J Bacteriol 184: 867-870.
34. Pringle, J.R., Adams, A.E., Drubin, D.G., and Haarer, B.K. (1991) Immunofluorescence methods for yeast. Methods Enzymol 194: 565-165.
35. Skarstad, K., Boye, E., and Steen, H.B. (1986) Timing of initiation of chromosome replication in individual Escherichia coli cells. EMBO J 5: 1711-1717.
36. Skarstad, K., Lueder, G., Lurz, R., Speck, C., and Messer, W. (2000) The Escherichia coli SeqA protein binds specifically and co-operatively to two sites in hemimethylated and fully methylated oriC. Mol Microbiol 36: 1319-1326.
37. Slater, S., Wold, S., Lu, M., Boye, E., Skarstad, K., and Kleckner, N. (1995) E. coli SeqA protein binds oriC in two different methylmodulated reactions appropriate to its roles in DNA replication initiation and origin sequestration. Cell 82: 927-936.
38. Sunako, Y., Onogi, T., and Hiraga, S. (2001) Sister chromosome cohesion of Escherichia coli. Mol Microbiol 42: 1233-1241.
39. Taghbalout, A., Landoulsi, A., Kern, R., Yamazoe, M., Hiraga, S., Holland, B., et al. (2000) Competition between the replication initiator DnaA and the sequestration factor SeqA for binding to the hemimethylated chromosomal origin of E Coli in vitro. Genes Cell 5: 873-884.
40. Withers, H.L., and Bernander, R. (1998) Characterization of dnaC2 and dnaC28 mutants by flow cytometry. J Bacteriol 180: 1624-1631.
41. Yamanaka, K., Ogura, T., Niki, H., and Higara, S. (1996) Identification of two new genes, mukE, mukF, involved in chromosome partitioning in Escherichia coli. Mol Gen Genet 250: 241-251.