Localization of the NBMPR-sensitive equilibrative nucleoside transporter, ENT1, in the rat dorsal root ganglion and lumbar spinal cord

Brain Research

Volumn 1059, Issue 2, 2005, Pages 129-138

  Governo, Ricardo J M ^a,b   Deuchars, Jim ^a   Baldwin, Stephen A ^a   King, Anne E ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Adenosine; Nociception; Nucleoside pain; Spinal cord; Synaptic transmission; Transporter

Indexed keywords

ADENOSINE; ANTIBODY; EQUILIBRATIVE NUCLEOSIDE TRANSPORTER 1; NITROBENZYLTHIOINOSINE; NUCLEAR FACTOR; PEROXIDASE; SUBSTANCE P;

ANIMAL CELL; ANIMAL TISSUE; ANTIBODY LABELING; ARTICLE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ELECTRON MICROSCOPY; GLIA; IMMUNOBLOTTING; IMMUNOFLUORESCENCE; LUMBAR SPINAL CORD; MYELINATED NERVE; NONHUMAN; NONMYELINATED NERVE; PEPTIDERGIC NERVOUS SYSTEM; PERIKARYON; POSTSYNAPTIC POTENTIAL; PRESYNAPTIC POTENTIAL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; RAT; SENSORY GANGLION; SPINAL CORD DORSAL HORN; SPINAL GANGLION; SUBSTANTIA GELATINOSA; TISSUE HOMOGENATE;

ANIMALS; CARRIER PROTEINS; GANGLIA, SENSORY; GANGLIA, SPINAL; IMMUNOHISTOCHEMISTRY; LUMBAR VERTEBRAE; NEUROGLIA; NEURONS; NUCLEOSIDE TRANSPORT PROTEINS; POSTERIOR HORN CELLS; RATS; RATS, WISTAR; SPINAL CORD; THIOINOSINE; TISSUE DISTRIBUTION;

EID: 26844574059     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.brainres.2005.08.017     Document Type: Article

References (53)

1. M.A. Ackley, R.J. Governo, C.E. Cass, J.D. Young, S.A. Baldwin, and A.E. King Control of glutamatergic neurotransmission in the rat spinal dorsal horn by the nucleoside transporter ENT1 J. Physiol. 548 2003 507 517
2. C.M. Anderson, W. Xiong, J.D. Geiger, J.D. Young, C.E. Cass, S.A. Baldwin, and F.E. Parkinson Distribution of equilibrative, nitrobenzylthioinosine-sensitive nucleoside transporters (ENT1) in brain J. Neurochem. 73 1999 867 873
3. L. Atkinson, T.F. Batten, and J. Deuchars P2X(2) receptor immunoreactivity in the dorsal vagal complex and area postrema of the rat Neuroscience 99 2000 683 696
4. S.A. Baldwin, J.R. Mackey, C.E. Cass, and J.D. Young Nucleoside transporters: molecular biology and implications for therapeutic development Mol. Med. Today 5 1999 216 224
5. E. Bastia, K. Varani, A. Monopoli, and R. Bertorelli Effects of A(1) and A(2A) adenosine receptor ligands in mouse acute models of pain Neurosci. Lett. 328 2002 241 244
6. Z.A. Berry, Immunological approaches to the study of mammalian nucleoside transporters, PhD thesis, School of Biochemistry, University of Leeds, 2001.
7. K.M. Braas, A.C. Newby, V.S. Wilson, and S.H. Snyder Adenosine-containing neurons in the brain localized by immunocytochemistry J. Neurosci. 6 1986 1952 1961
8. R.E. Brooke, J. Deuchars, and S.A. Deuchars Input-specific modulation of neurotransmitter release in the lateral horn of the spinal cord via adenosine receptors J. Neurosci. 24 2004 127 137
9. R.A. Cunha Adenosine as a neuromodulator and as a homeostatic regulator in the nervous system: different roles, different sources and different receptors Neurochem. Int. 38 2001 107 125
10. J.I. Choca, H.K. Proudfit, and R.D. Green Identification of A1 and A2 adenosine receptors in the rat spinal cord J. Pharmacol. Exp. Ther. 242 1987 905 910
11. N. Dale Delayed production of adenosine underlies temporal modulation of swimming in frog embryo J. Physiol. 511 Pt. 1 1998 265 272
12. G.E. DeLander, and C.J. Hopkins Involvement of A2 adenosine receptors in spinal mechanisms of antinociception Eur. J. Pharmacol. 139 1987 215 223
13. S.A. Deuchars, R.E. Brooke, and J. Deuchars Adenosine A1 receptors reduce release from excitatory but not inhibitory synaptic inputs onto lateral horn neurons J. Neurosci. 21 2001 6308 6320
14. T.V. Dunwiddie, and L. Diao Extracellular adenosine concentrations in hippocampal brain slices and the tonic inhibitory modulation of evoked excitatory responses J. Pharmacol. Exp. Ther. 268 1994 537 545
15. T.V. Dunwiddie, and S.A. Masino The role and regulation of adenosine in the central nervous system Annu. Rev. Neurosci. 24 2001 31 55
16. S.R. Fam, C.J. Gallagher, and M.W. Salter P2Y(1) purinoceptor-mediated Ca(2+) signaling and Ca(2+) wave propagation in dorsal spinal cord astrocytes J. Neurosci. 20 2000 2800 2808
17. J.D. Geiger, and J.I. Nagy Heterogeneous distribution of adenosine transport sites labelled by [3H]nitrobenzylthioinosine in rat brain: an autoradiographic and membrane binding study Brain Res. Bull. 13 1984 657 666
18. J.D. Geiger, and J.I. Nagy Localization of [3H]nitrobenzylthioinosine binding sites in rat spinal cord and primary afferent neurons Brain Res. 347 1985 321 327
19. J.D. Geiger, F.S. LaBella, and J.I. Nagy Characterization of nitrobenzylthioinosine binding to nucleoside transport sites selective for adenosine in rat brain J. Neurosci. 5 1985 735 740
20. D.A. Griffith, and S.M. Jarvis Nucleoside and nucleobase transport systems of mammalian cells Biochim. Biophys. Acta 1286 1996 153 181
21. M. Griffiths, S.Y. Yao, F. Abidi, S.E. Phillips, C.E. Cass, J.D. Young, and S.A. Baldwin Molecular cloning and characterization of a nitrobenzylthioinosine-insensitive (ei) equilibrative nucleoside transporter from human placenta Biochem. J. 328 1997 739 743
22. M. Griffiths, N. Beaumont, S.Y. Yao, M. Sundaram, C.E. Boumah, A. Davies, F.Y. Kwong, I. Coe, C.E. Cass, J.D. Young, and S.A. Baldwin Cloning of a human nucleoside transporter implicated in the cellular uptake of adenosine and chemotherapeutic drugs Nat. Med. 3 1997 89 93
23. J.G. Gu, A. Nath, and J.D. Geiger Characterization of inhibitor-sensitive and -resistant adenosine transporters in cultured human fetal astrocytes J. Neurochem. 67 1996 972 977
24. D.J. Hakes, and J.E. Dixon New vectors for high level expression of recombinant proteins in bacteria Anal. Biochem. 202 1992 293 298
25. F.Y. Kwong, J.S. Wu, M.M. Shi, H.E. Fincham, A. Davies, P.J. Henderson, S.A. Baldwin, and J.D. Young Enzymic cleavage as a probe of the molecular structures of mammalian equilibrative nucleoside transporters J. Biol. Chem. 268 1993 22127 22134
26. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
27. L.J. Lao, E. Kumamoto, C. Luo, H. Furue, and M. Yoshimura Adenosine inhibits excitatory transmission to substantia gelatinosa neurons of the adult rat spinal cord through the activation of presynaptic A(1) adenosine receptor Pain 94 2001 315 324
28. L.J. Lao, Y. Kawasaki, K. Yang, T. Fujita, and E. Kumamoto Modulation by adenosine of Adelta and C primary-afferent glutamatergic transmission in adult rat substantia gelatinosa neurons Neuroscience 125 2004 221 231
29. S. Latini, and F. Pedata Adenosine in the central nervous system: release mechanisms and extracellular concentrations J. Neurochem. 79 2001 463 484
30. S.N. Lawson, A.A. Harper, E.I. Harper, J.A. Garson, and B.H. Anderton A monoclonal antibody against neurofilament protein specifically labels a subpopulation of rat sensory neurones J. Comp. Neurol. 228 1984 263 272
31. T. Liu, T. Fujita, Y. Kawasaki, and E. Kumamoto Regulation by equilibrative nucleoside transporter of adenosine outward currents in adult rat spinal dorsal horn neurons Brain Res. Bull. 64 2004 75 83
32. J.R. Mackey, S.A. Baldwin, D. Young, and C.E. Cass Nucleoside transport and its significance for anticancer drug resistance Drug Resist. Upd. 1 1998 310 324
33. P. Meghji, J.B. Tuttle, and R. Rubio Adenosine formation and release by embryonic chick neurons and glia in cell culture J. Neurochem. 53 1989 1852 1860
34. H. Musa, H. Dobrzynski, Z. Berry, F. Abidi, C.E. Cass, J.D. Young, S.A. Baldwin, and M.R. Boyett Immunocytochemical demonstration of the equilibrative nucleoside transporter rENT1 in rat sinoatrial node J. Histochem. Cytochem. 50 2002 305 309
35. M.E. Olah, and G.L. Stiles The role of receptor structure in determining adenosine receptor activity Pharmacol. Ther. 85 2000 55 75
36. D.H. Patel, C.R. Crawford, C.W. Naeve, and J.A. Belt Cloning, genomic organization and chromosomal localization of the gene encoding the murine sodium-dependent, purine-selective, concentrative nucleoside transporter (CNT2) Gene 242 2000 51 58
37. M.K. Patel, R.D. Pinnock, and K. Lee Adenosine exerts multiple effects in dorsal horn neurones of the adult rat spinal cord Brain Res. 920 2001 19 26
38. M.W. Ritzel, S.Y. Yao, A.M. Ng, J.R. Mackey, C.E. Cass, and J.D. Young Molecular cloning, functional expression and chromosomal localization of a cDNA encoding a human Na+/nucleoside cotransporter (hCNT2) selective for purine nucleosides and uridine Mol. Membr. Biol. 15 1998 203 211
39. M.W. Ritzel, A.M. Ng, S.Y. Yao, K. Graham, S.K. Loewen, K.M. Smith, R.G. Ritzel, D.A. Mowles, P. Carpenter, X.Z. Chen, E. Karpinski, R.J. Hyde, S.A. Baldwin, C.E. Cass, and J.D. Young Molecular identification and characterization of novel human and mouse concentrative Na+-nucleoside cotransporter proteins (hCNT3 and mCNT3) broadly selective for purine and pyrimidine nucleosides (system cib) J. Biol. Chem. 276 2001 2914 2927
40. J. Sawynok Adenosine receptor activation and nociception Eur. J. Pharmacol. 347 1998 1 11
41. J. Sawynok, and X.J. Liu Adenosine in the spinal cord and periphery: release and regulation of pain Prog. Neurobiol. 69 2003 313 340
42. G. Schulte, B. Robertson, B.B. Fredholm, G.E. DeLander, P. Shortland, and C. Molander Distribution of antinociceptive adenosine A1 receptors in the spinal cord dorsal horn, and relationship to primary afferents and neuronal subpopulations Neuroscience 121 2003 907 916
43. A.M. Sebastiao, and J.A. Ribeiro Adenosine A2 receptor-mediated excitatory actions on the nervous system Prog. Neurobiol. 48 1996 167 189
44. J.C. Shryock, and L. Belardinelli Adenosine and adenosine receptors in the cardiovascular system: biochemistry, physiology, and pharmacology Am. J. Cardiol. 79 1997 2 10
45. C.J. Sinclair, A.E. Powell, W. Xiong, C.G. LaRiviere, S.A. Baldwin, C.E. Cass, J.D. Young, and F.E. Parkinson Nucleoside transporter subtype expression: effects on potency of adenosine kinase inhibitors Br. J. Pharmacol. 134 2001 1037 1044
46. W.D. Snider, and S.B. McMahon Tackling pain at the source: new ideas about nociceptors Neuron 20 1998 629 632
47. A. Sollevi Adenosine for pain control Acta Anaesthesiol. Scand., Suppl. 110 1997 135 136
48. M. Sundaram, S.Y. Yao, J.C. Ingram, Z.A. Berry, F. Abidi, C.E. Cass, S.A. Baldwin, and J.D. Young Topology of a human equilibrative, nitrobenzylthioinosine (NBMPR)-sensitive nucleoside transporter (hENT1) implicated in the cellular uptake of adenosine and anti-cancer drugs J. Biol. Chem. 276 2001 45270 45275
49. R.J. Traub, B. Allen, E. Humphrey, and M.A. Ruda Analysis of calcitonin gene-related peptide-like immunoreactivity in the cat dorsal spinal cord and dorsal root ganglia provide evidence for a multisegmental projection of nociceptive C-fiber primary afferents J. Comp. Neurol. 302 1990 562 574
50. J.S. Wu, F.Y. Kwong, S.M. Jarvis, and J.D. Young Identification of the erythrocyte nucleoside transporter as a band 4.5 polypeptide. Photoaffinity labeling studies using nitrobenzylthioinosine J. Biol. Chem. 258 1983 13745 13751
51. S.Y. Yao, A.M. Ng, M.W. Ritzel, W.P. Gati, C.E. Cass, and J.D. Young Transport of adenosine by recombinant purine- and pyrimidine-selective sodium/nucleoside cotransporters from rat jejunum expressed in Xenopus laevis oocytes Mol. Pharmacol. 50 1996 1529 1535
52. S.Y. Yao, A.M. Ng, W.R. Muzyka, M. Griffiths, C.E. Cass, S.A. Baldwin, and J.D. Young Molecular cloning and functional characterization of nitrobenzylthioinosine (NBMPR)-sensitive (es) and NBMPR-insensitive (ei) equilibrative nucleoside transporter proteins (rENT1 and rENT2) from rat tissues J. Biol. Chem. 272 1997 28423 28430
53. J.D. Young, C.I. Cheeseman, J.R. Mackey, C.E. Cass, and S.A. Baldwin Molecular mechanisms of nucleoside and nucleoside drug transport Gastrointestinal Transport 2000 Academic Press San Diego 378 pp.