Mutagenesis of glycine 179 modulates both catalytic efficiency and reduced pyridine nucleotide specificity in cytochrome b5 reductase

Biochemistry

Volumn 44, Issue 41, 2005, Pages 13467-13476

  Roma, Glenn W ^a   Crowley, Louis J ^a   Davis, C Ainsley ^a   Barber, Michael J ^a  

^a UNIVERSITY OF SOUTH FLORIDA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; CYTOLOGY; ENZYMES; OXIDATION; REDUCTION; STOICHIOMETRY;

CATALYTIC EFFICIENCY; CYTOCHROME; GLYCINE; TRANSHYDROGENASES;

MUTAGENESIS;

CYTOCHROME B5 REDUCTASE; FERREDOXIN NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE REDUCTASE; FLAVINE ADENINE NUCLEOTIDE; GLYCINE; NICOTINAMIDE ADENINE DINUCLEOTIDE; OLIGONUCLEOTIDE; PYRIDINE NUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

AMINO ACID SEQUENCE; ARTICLE; BINDING AFFINITY; CATALYSIS; ENZYME ACTIVITY; ENZYME KINETICS; ESCHERICHIA COLI; HUMAN; MUTAGENESIS; NONHUMAN; POTENTIOMETRY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN MOTIF; PROTEIN SECONDARY STRUCTURE; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY; ULTRAVIOLET SPECTROSCOPY;

AMINO ACID SEQUENCE; ANIMALS; CYTOCHROME-B(5) REDUCTASE; ENZYME STABILITY; ESCHERICHIA COLI; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NAD; RATS; SEQUENCE ALIGNMENT; SPECTROPHOTOMETRY, ULTRAVIOLET; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI;

EID: 26644470345     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi051165t     Document Type: Article

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